From dangl from email.unc.edu Fri Aug 1 16:58:30 2008 From: dangl from email.unc.edu (Jeff Dangl) Date: Fri Aug 1 18:44:15 2008 Subject: [Arabidopsis] comparative pathogenomics positions Message-ID: <48938706.2030402@email.unc.edu> Subject heading: Post-doc position in comparative genomics and evolution of host specialization and virulence at UNC-Chapel Hill We are using new whole genome sequencing technologies to study the evolution of host-specialization and virulence in Pseudomonas syringae pathovars. This is a NIH funded collaboration between Dr. Jeff Dangl and Dr. Corbin D. Jones. We seek a highly motivated post-doc interested in merging computational and experimental biology. Ideal candidates will have a strong evolutionary background and experience bioinformatics, programming, or genome assembly. The University of North Carolina at Chapel Hill is a diverse campus located in the Research Triangle of North Carolina. Both North Carolina State University and Duke University are close by. UNC-CH is repeatedly ranked one of the best public Universities and a great place to post-doc. Candidates should contact Corbin Jones at cdjones@ email.unc.edu Bioinformatics and Computational Biology Grad Student Position Project Description: Develop and apply novel algorithms for whole genome assembly using new high throughput genome sequencing technologies. We are using new whole genome sequencing technologies to study the evolution of host-specialization and virulence in Pseudomonad pathovars. This is a NIH funded collaboration between Dr. Jeff L. Dangl and Dr. Corbin D. Jones. We seek highly motivated graduate students interested in developing approaches and software tools for the assembly of bacterial genomes using new short read sequencing technology. In contrast to traditional approaches to genome sequencing, which are quite expensive, several new technologies—e.g. Roche/454 and Illumina/Solexa Genome Analyzer—have been developed that produce shorter more error prone DNA sequence at greatly reduced cost. As the throughput and quality of these new technologies improves, they will likely supplant existing genome sequencing technologies. Currently, there is a great need for software tools to analyze these data. The unique characteristics of these data, however, prevent rote application of traditional methods. Thus, there is a critical need to develop novel computational approaches to working with these new technologies. The Jones and Dangl labs are looking for a graduate student to be co- advised on this project. No prior experience with genome assembly required, only motivated, enthusiastic students are needed. We’ll teach you what you need to know. Candidates should apply to the BBSP Graduate Program at: http://www.med.unc.edu/bbsp/ Websites: http://www.bio.unc.edu/Faculty/CDJones/Lab/ http://www.bio.unc.edu/dangl/lab/ Related publications: Jeck WR, Reinhardt JA, Baltrus DA, Hickenbotham MT, Magrini V, Mardis ER, Dangl JL, Jones CD. 2007. Extending assembly of short DNA sequences to handle error. Bioinformatics. 2007 Sep 24; Grant SR, Fisher EJ, Chang JH, Mole BM, Dangl JL. 2006. Subterfuge and manipulation: type III effector proteins of phytopathogenic bacteria. Annu Rev Microbiol.60:425-49. Jones JD, Dangl JL. 2006. The plant immune system. Nature. Nov 16;444(7117):323-9. -------------------------------- Corbin D. Jones, Ph.D. Department of Biology Carolina Center for Genome Sciences Campus Box 3280, Coker Hall UNC-Chapel Hill Chapel Hill, NC 27599-3280 --------------------------------- Jeff Dangl, PhD. Dept. of Biology, CB#3280 Coker Hall Rm. 108 Univ of North Carolina Chapel Hill, NC 27599-3280 ph 919-962-5624 fax 919-962-1625 web http://www.bio.unc.edu/faculty/dangl/ From simonmi1 from post.tau.ac.il Sun Aug 3 07:57:50 2008 From: simonmi1 from post.tau.ac.il (simonmi1@post.tau.ac.il) Date: Sun Aug 3 23:21:01 2008 Subject: [Arabidopsis] Can un-fused GFP enter the mitochondria? Message-ID: <20080803155750.14043acxj04dbuck@webmail.tau.ac.il> Dear all, it is common knowledge that the fluorescence of over-expressed, un-fused GFP (35S::GFP) can be easily detected in the cytoplasm and nucleus of plant cells. Does anyone know whether this free GFP enters the mitochondria as well? Thanks for your response is advance, Simon From dmolino from versailles.inra.fr Mon Aug 4 04:48:50 2008 From: dmolino from versailles.inra.fr (dmolino@versailles.inra.fr) Date: Mon Aug 4 12:09:37 2008 Subject: [Arabidopsis] Can un-fused GFP enter the mitochondria? In-Reply-To: <20080803155750.14043acxj04dbuck@webmail.tau.ac.il> References: <20080803155750.14043acxj04dbuck@webmail.tau.ac.il> Message-ID: <1217843330.4896d082da8b5@www.versailles.inra.fr> Salut Simon, Absolutely not, a signal is needed to enter the mitocondria. But if for some reasons your mitocondria get sick... you could see some artefacts. Selon simonmi1@post.tau.ac.il: Diana > Dear all, > it is common knowledge that the fluorescence of over-expressed, > un-fused GFP (35S::GFP) can be easily detected in the cytoplasm and > nucleus of plant cells. Does anyone know whether this free GFP enters > the mitochondria as well? > Thanks for your response is advance, > Simon > > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > Diana Molino Laboratoire de Biologie Cellulaire INRA Versailles Route de St-Cyr (RD10) F-78026 VERSAILLES Cedex Tel: +33 1 30 83 31 13 Fax: +33 1 30 83 30 99 From tps from duke.edu Tue Aug 5 15:32:21 2008 From: tps from duke.edu (Tai-Ping Sun) Date: Wed Aug 6 12:01:06 2008 Subject: [Arabidopsis] POSTDOC POSITION Message-ID: POSTDOCTORAL POSITION: Gibberellin Signaling in Arabidopsis Department of Biology, Duke University A postdoctoral position is available immediately to study "Gibberellin Signaling in Arabidopsis." We are looking for a highly motivated individual who has an excellent background in protein chemistry, genetics, genomics, molecular biology or plant development. Bioactive gibberellins (GAs) are a class of plant hormones that modulate diverse developmental processes throughout the life cycle of a plant. The nuclear DELLA proteins are highly conserved negative regulators of GA signaling that act immediately downstream of the GA receptors GID1 to repress GA-mediated growth in Arabidopsis and in a number of crops. GA signal appears to de-repress its response pathway by triggering degradation of these repressor proteins. To understand how GA controls plant development, we are using biochemical and genomics approaches to elucidate the signaling events immediately downstream of DELLA. Related publications: Dill et al. (2004) The Arabidopsis F-box protein SLEEPY1 targets GA signaling repressors for GA-induced degradation. Plant Cell, 16, 1392-1405. Sun and Gubler (2004) Molecular mechanism of gibberellin signaling in plants. Annu. Rev. Plant Biol. 55, 197-223. Griffiths et al. (2006) Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis. Plant Cell, 18, 3399-3414. Zentella et al. (2007) Global analysis of DELLA direct targets in early gibberellin signaling in Arabidopsis. Plant Cell 19, 3037-3057. Please send (by e-mail) a cover letter containing your research summary and career goals, curriculum vitae, and names, e-mail addresses, and phone numbers of three references to: Tai-ping Sun e-mail address: tps@duke.edu Thanks, ------------------------------------------------------------ Tai-ping Sun Professor Department of Biology French 3104, Box 90338 Science Dr. ( -- for FedEx delivery) Duke University Durham, NC 27708 email: tps@duke.edu http://fds.duke.edu/db/aas/Biology/faculty/tps From clchiham from ucdavis.edu Wed Aug 6 11:32:17 2008 From: clchiham from ucdavis.edu (CECILIA CHI-HAM) Date: Wed Aug 6 12:01:12 2008 Subject: [Arabidopsis] Please Post: Job Ad Message-ID: Please post the following job ad: Seeking: Scientist interested in technology transfer, intellectual property, regulatory and policy. The Public Intellectual Property Resource for Agriculture (PIPRA) is a grant-supported program that coordinates agricultural intellectual property (IP) management across 50 member institutions to enable research projects that address specialty crops and/or humanitarian projects in developing countries. PIPRA manages a comprehensive patent database comprising intellectual property from all member institutions, creates and delivers educational programs to support intellectual property management capacity building in developing countries, conducts legal research to assess the freedom to operate around specific patented agricultural technologies and implements collaborative strategies to manage pools of intellectual property belonging to multiple institutional owners. There are four functional units within PIPRA: IP Information Services and Analysis, Education and Outreach, Biotechnology Resources, and Intellectual Property management. This position (IP Analyst) falls within the IP Information Services and Analysis unit, and was created to provide analytical services in support of legal research on various projects undertaken by this unit. Under the direction of the unit director, this position independently works with internal staff and external organizations to ensure that PIPRA fulfills all requested contractual services in a timely manner and provides quality analytical data. The analyst will participate in the following projects: -assess the biofuel IP landscape associated with the use of lignocellulosic biomass. -provide analytical support for legal IP freedom-to-operate opinions of specific technologies. - review the US and international biosafety regulatory process as it relates to specialty crops. Analyses to be conducted will typically require cross-disciplinary awareness of law, science, public policy, and information technology. The analyst will independently set priorities, milestones, and presents deliverables in written and/or oral format . Time: 40 hours per week Ideal candidates will meet the following criteria: Education: University degree is required- a degree in biological sciences, plant biotechnology, biochemistry, or related field is strongly preferred. Patent agent certification or understanding of legal IP issues is highly desired but NOT required. Understanding or interest in learning about legal IP and regulatory issues is required. Skills: * Demonstrated experience in project management and research, especially as it relates to IP law, science, or policy. * Demonstrated ability to work independently. * Ability to professionally interact with representatives of external organizations to fulfill requests and provide support. * Ability to design and supervise implementation of a variety of projects related to IP management. * Demonstrated experience in oral and written communications with a broad section of professionals in the legal, academic, and business communities. * Ability to support development of institutional strategies addressing complex issues involving IP law or policy. * Demonstrated skills in assessing priorities and strong organizational skills to effectively work on multiple projects simultaneously. * Demonstrated skill in data management and analysis. * Familiarity or ability to learn about, IP, scientific literature, and other pertinent databases. * Excellent writing and speaking skills. * Ability to work effectively individually and as part of a team. * Professional skills, judgment and diplomacy to achieve successful outcomes in sensitive or difficult interactions. * Computer skills in spreadsheets This position offers a competitive salary determined by the successful candidate's experience and full health benefits. Please send a statement describing your interests, detailed curriculum vitae, and 2-3 letters of references via email to Kathleen Bess, klbess@ucdavis.edu. Review of applications will start August 15th, 2008 and continue until the position is filled. The University of California, Davis is an equal opportunity/affirmative action employer. From kv35 from cornell.edu Fri Aug 8 12:05:09 2008 From: kv35 from cornell.edu (Klaas van Wijk) Date: Sun Aug 10 15:44:01 2008 Subject: [Arabidopsis] Postdoctoral Position in Maize/Rice Protein Biochemistry and Proteomics at Cornell University Message-ID: <6.2.1.2.2.20080808130033.0304e650@postoffice6.mail.cornell.edu> Postdoctoral Position in Maize/Rice Protein Biochemistry and Proteomics van Wijk lab, Cornell University, Ithaca, NY, USA. [1]http://cbsu.tc.cornell.edu/vanwijk/ Experienced Plant Protein Biochemist A postdoctoral position (2-3 years) is available in Plant Protein Biochemistry and Proteomics to work on a project sponsored by the NSF-Genome program. This project concerns a cell-type specific comparative analysis of C3 and C4 leaf development in Rice, Sorghum and Maize. In leaves of such C4 grasses photosynthetic activities are partitioned between two morphologically and biochemically distinct bundle sheath (BS) and mesophyll (M) cells. The objectives are to compare the quantitative and qualitative patterns of transcripts, proteins, metabolic activities and anatomy at discrete stages and in specific cell types in rice (C3), maize and sorghum (C4), and to develop a model of the regulatory networks governing C4 anatomy and function. These experimental datasets will be linked by new informatics tools for comparative transcriptional and proteome analysis, to provide the foundation for a systems biology approach to understanding C4 biology. The responsibilities of the postdoc include isolation of cell-type specific proteomes, protein separation and proteome analysis, as well as biological interpretation of these large scale datasets. Experience in plant protein biochemistry (with a proven track record) and a strong interest in plant metabolism, leaf growth, development and systems biology is required. Experience with nanoLC-Electrospray tandem Mass Spectrometry (e.g. LTQ, Q-TOF or LTQ-Orbitrap) is beneficial. The postdoc will work closely together with other team members, including mass spectrometry specialists and bioinformaticians. Salary will be commensurate with experience with generous employee benefits according the Cornell Human Resource Office. Review of applications will begin immediately, and continue until the position is filled. Application Instructions: Send your curriculum vitae, a statement of research interests and career goals, representative publications, and three letters of reference by email to: [2]kv35@cornell.edu Klaas J. van Wijk, Associate Professor, Dept of Plant Biology, Cornell University, Ithaca, NY 14853, USA; tel +1-607-255-3664; [3]http://cbsu.tc.cornell.edu/vanwijk/ Relevant papers from the van Wijk lab: Majeran W., Zybailov B., Ytterberg A.J., Dunsmore J., Sun Q.and van Wijk K.J. (2008) Consequences of C4 differentiation for chloroplast membrane proteomes in maize mesophyll and bundle sheath cells. Mol Cell Proteomics - pre-publication online - Covshoff S., Majeran W., Liu P., Kolkman J.M., van Wijk K.J.and Brutnell T.P. (2008) Deregulation of maize c4 photosynthetic development in a mesophyll cell-defective mutant. Plant Physiol 146: 1469-1481. Majeran W., Cai Y., Sun Q.and van Wijk K.J. (2005) Functional Differentiation of Bundle Sheath and Mesophyll Maize Chloroplasts Determined by Comparative Proteomics. The Plant Cell 17: 3111-3140. Klaas J. van Wijk Associate Professor Director of Graduate Studies (DGS) for the Field of Plant Biology Dept of Plant Biology 332 Emerson Hall Cornell University Ithaca, NY 14853, USA tel +1-607-255-3664 [4]http://cbsu.tc.cornell.edu/vanwijk/ References 1. http://cbsu.tc.cornell.edu/vanwijk/ 2. mailto:kv35@cornell.edu 3. http://cbsu.tc.cornell.edu/vanwijk/ 4. http://cbsu.tc.cornell.edu/vanwijk/ From anirbanb from ncbs.res.in Sat Aug 9 13:02:52 2008 From: anirbanb from ncbs.res.in (Anirban Baral) Date: Sun Aug 10 15:44:09 2008 Subject: [Arabidopsis] By 2 transfection by cocultivation Message-ID: <60178.192.168.1.1.1218304972.squirrel@mail.ncbs.res.in> Hi Arabineters, Can anyone please suggest me a protocol for BY2 cell transfection by Agrobacterium co cultivation.Should I use auxins like NAA,IAA etc in plating media? Thanks in advance Anirban anirbanb@ncbs.res.in -- Anirban Baral JUNIOR SCHOLAR NATIONAL CENTRE FOR BIOLOGICAL SCIENCES TATA INSTITUTE OF FUNDAMENTAL RESEARCH UAS-GKVK CAMPUS,BELLARY ROAD BANGALORE-560065,INDIA From chidubt from me.com Sun Aug 10 22:36:12 2008 From: chidubt from me.com (chida NK) Date: Tue Aug 12 15:21:36 2008 Subject: [Arabidopsis] How to spray phyto hormones!!!! Message-ID: <4C26E8D7-8366-4817-98C4-1315C9D68835@me.com> Dear Colleagues.. I would like to know how we have to spray the JA, ABA and Ethapon (ethylene) on Arabidopsis plants. I have to make gene expression analysis after the spray with these hormones. It will be great if anybody share there experience regarding the preparation of the solution. I appreciate your time. sincerely chida From Jose.Gonzalez from sdstate.edu Mon Aug 11 12:23:21 2008 From: Jose.Gonzalez from sdstate.edu (Gonzalez, Jose) Date: Tue Aug 12 15:21:44 2008 Subject: [Arabidopsis] Research Scientist Position at South Dakota State university Message-ID: <5323AEB9BA07874090D896D29441D6C601E50F7F@ATLANTIS-EVS.jacks.local> SDSU FUNCTIONAL GENOMICS CORE FACILITY Research Scientist College of Agriculture and Biological Sciences South Dakota State University RESPONSIBILITIES: The College of Agriculture and Biological Sciences at South Dakota State University seeks to fill the position of Research Scientist of the new Functional Genomics Core Facility (FGCF) housed in the Northern Plains Biostress Laboratory. The SDSU-FGCF exists to facilitate interdisciplinary gene function research and training in viral, bacterial, plant and animal systems. The FGCF consists of 6 integrated laboratories (Genomics, Automated Genomics, Gel & Microplate Analysis, MicroImaging, General Tissue Prep and Histological Tissue Prep), each containing several instruments (listed at http://genomics.sdstate.edu/). A multi-department governing committee and a faculty director administer the FGCF. Responsibilities of the Research Scientist include (but are not limited to): coordinating daily FGCF operations, including supervision of one technician; defining and implementing standard operating procedures and maintenance for each instrument; providing group and individual training on FGCF policies and the proper use of each instrument, including data analysis; maintaining the FGCF website; managing the billing system, providing informational and training sessions, and providing analytical services to university and industry users. MINIMUM QUALIFICATIONS: A Ph.D in biology, microbiology, biochemistry, molecular biology, or a related area; research training and experience with DNA, RNA and protein quantification, resolving, visualizing and analysis; RT-PCR, practical experiences in a molecular-biology, multi-user facility and computer tech support of molecular biology equipment. PREFERRED QUALIFICATIONS: Research experience in fluorescence and confocal microscopy, microtomy, laser capture systems, DNA sequencers, and automated equipment such as liquid handling robots; strong computer skills, including experience with use of bioinformatic databases. SALARY: Salary is commensurate with qualifications. UNIVERSITY/COMMUNITY: SDSU is a land grant institution and the state's largest institution of higher education with an enrollment of approximately 11,000 students and strong growth in research activity. The FGCF has members from several departments and Centers on campus including Plant Science, Biology and Microbiology, Veterinary Science, Pharmaceutical Science, Animal and Range Science, Dairy Science, Nutrition, Food Science and Hospitality, Wildlife and Fisheries Science, Agricultural Engineering, The Center for Infectious Disease Research and Vaccinology, Center of Excellence in Drought Tolerance and the Center for Bioprocessing Research and Development. SDSU is located in Brookings, South Dakota, a community of approximately 17,500 near the east central border of South Dakota on Interstate 29. The city has an excellent K-12 education system, is accessible to major medical facilities, has an active cultural and social environment, and has numerous lakes and parks within driving distance. It is 50 miles north of Sioux Falls, a city of 150,000. APPLICATION DEADLINE: Applications will be accepted until September 2, 2008, or until a suitable candidate is identified. APPLICATION PROCESS: SDSU accepts applications through an on-line employment site. To apply, visit: https://yourfuture.sdbor.edu, search by the position title, view the job announcement, and click on "apply for this posting." The system will guide you through the electronic application form. The employment site will require the attachment of a cover letter, resume, and reference page (Please submit at least 3 professional references). For full consideration, interested applicants must also attach a career interest statement detailing the applicant's philosophy of university molecular biology core facility administration. Please contact SDSU Human Resources at (605) 688-4128 if you require assistance with this process. For questions on the position, contact Dr. Volker Br?zel at volker.brozel@sdstate.edu or at (605)688-6483. South Dakota State University is committed to affirmative action, equal opportunity and the diversity of its faculty, staff and students. Women and minorities are encouraged to apply. Arrangements for accommodations required by disabilities can be made at TTY (605) 688-4394. SDSU prohibits discrimination on the basis of race, color, creed, religion, national origin, ancestry, gender, marital status, pregnancy, sexual orientation, age, disability, veteran's status or any other protected class in the offering of all educational programs and employment opportunities. Individuals with concerns regarding discrimination should contact: Equal Opportunity Officer/Title IX Coordinator, Human Resources, Administration 324, SDSU, Brookings, SD 57007. Phone: (605) 688-4128. Jos? L. Gonz?lez Jose L. Gonzalez Assistant Professor. Dept. of Plant Sciences South Dakota State University Brookings, SD 57007 USA Phone (office):605-688-6907 Fax: 605-688-4452 http://plantsci.sdstate.edu/gonzalez/gonzalez lab.htm From reddy from lamar.colostate.edu Mon Aug 11 16:18:24 2008 From: reddy from lamar.colostate.edu (ASN Reddy) Date: Tue Aug 12 15:21:50 2008 Subject: [Arabidopsis] Postdoctoral Position Message-ID: Postdoctoral Position Department of Biology and Program in Molecular Plant Biology Colorado State University, Fort Collins, CO 80523 A postdoctoral position is available in the Department of Biology and Program in Molecular Plant Biology to study nuclear pre-mRNA processing and its regulation in plants. A Ph.D. in plant molecular biology or a related field is required. Applicants must have experience in standard biochemical and molecular techniques. Experience with functional genomics approaches (reverse genetics, microarrays and proteomics) and bioinformatics tools is desirable. The successful candidate will develop and apply new and existing techniques to isolate protein complexes involved in pre-mRNA splicing, identify components of these complexes using proteomic approaches and identify mRNA targets of serine/arginine-rich proteins. This project will provide opportunities to perform global studies using high throughput technologies involving next generation of sequencing technologies. The incumbent will have an opportunity to participate in other ongoing projects in the laboratory and is expected to interact with other post-docs, graduate and undergraduate students in the laboratory. Lab publications in pre-mRNA splicing area include: Annu. Rev. Plant Biol. 58: 267-294 (2007); Plant J. 49: 1091-1107 (2007); PLoS ONE, 5: e471, Pages 1-13 (2007); BMC Genomics 10: 9:159; pages 1-11 (2008); J. Cell Sci. 119: 3527-3538 (2006); Trends in Plant Science 9: 541-547 (2004); Plant. J. 36: 883-893 (2003); J. Biol. Chem. 274: 36428-36438(1999); Plant Cell 10: 1637-1647 (1998); Plant Cell 8: 1421-1435 (1996). For more information on our ongoing research projects and other publications please visit http://lamar.colostate.edu/~reddy/. Salary commensurate with qualifications and research experience. Please send (preferably by e-mail) curriculum vitae, a brief statement of research experience, and three references to: Dr. A.S.N. Reddy, Professor Department of Biology and Program in Molecular Plant Biology Colorado State University Fort Collins, CO 80523-1878 Phone: 970-491-5773 FAX: 970-491-0649 Email: reddy@colostate.edu Colorado State University has the state of the art research facilities and excellent support services. CSU is located at the foot of the beautiful Rocky Mountains in the town of Fort Collins, which enjoys one of the best climates in the USA and is a perennial in the top 10 best places to live. Fort Collins has easy access to some of the best hiking, skiing, kayaking, and fishing places in North America. Colorado State University is an equal opportunity/affirmative action employer. -- A.S.N. Reddy Professor Department of Biology and Program in Molecular Plant Biology Colorado State University Fort Collins, CO 80523 Ph. No. 970-491-5773 Fax. No. 970-491-0649 http://lamar.colostate.edu/~reddy/index.html http://www.plantbiology.colostate.edu From reddy from lamar.colostate.edu Mon Aug 11 16:22:59 2008 From: reddy from lamar.colostate.edu (ASN Reddy) Date: Tue Aug 12 15:21:56 2008 Subject: [Arabidopsis] Postdoctoral Position to work on metabolic engineering of plants Message-ID: Postdoctoral Position Department of Biology and Program in Molecular Plant Biology Colorado State University, Fort Collins, CO 80523 A postdoctoral position is available in the Department of Biology and Program in Molecular Plant Biology to work on metabolic engineering of plants to produce novel chemicals. A Ph.D. in plant molecular biology or a related field is required. Applicants must have experience in standard biochemical and molecular techniques. Experience in metabolomics is desirable. The successful candidate will clone and express bacterial and/or fungal genes in plants and analyze transgenic plants for the presence of various chemicals. The incumbent will have an opportunity to participate in other ongoing projects in the laboratory and is expected to interact with other post-docs, graduate and undergraduate students in the laboratory. For more information on our ongoing research projects and other publications please visit http://lamar.colostate.edu/~reddy/. Salary commensurate with qualifications and research experience. Please send (preferably by e-mail) curriculum vitae, a brief statement of research experience, and three references to: Dr. A.S.N. Reddy, Professor Department of Biology and Program in Molecular Plant Biology Colorado State University Fort Collins, CO 80523-1878 Phone: 970-491-5773 FAX: 970-491-0649 Email: reddy@colostate.edu Colorado State University has the state of the art research facilities and excellent support services. CSU is located at the foot of the beautiful Rocky Mountains in the town of Fort Collins, which enjoys one of the best climates in the USA and is a perennial in the top 10 best places to live. Fort Collins has easy access to some of the best hiking, skiing, kayaking, and fishing places in North America. Colorado State University is an equal opportunity/affirmative action employer. A.S.N. Reddy Professor Department of Biology and Program in Molecular Plant Biology Colorado State University Fort Collins, CO 80523 Ph. No. 970-491-5773 Fax. No. 970-491-0649 http://lamar.colostate.edu/~reddy/index.html http://www.plantbiology.colostate.edu From stiwari from mendelbio.com Tue Aug 12 15:30:48 2008 From: stiwari from mendelbio.com (Shiv Tiwari) Date: Tue Aug 12 23:47:31 2008 Subject: [Arabidopsis] How to spray phyto hormones!!!! In-Reply-To: <4C26E8D7-8366-4817-98C4-1315C9D68835@me.com> References: <4C26E8D7-8366-4817-98C4-1315C9D68835@me.com> Message-ID: <48A1F2F8.2060009@mendelbio.com> I also need this info! Shiv chida NK wrote: > Dear Colleagues.. > I would like to know how we have to spray the JA, ABA and Ethapon > (ethylene) on Arabidopsis plants. I have to make gene expression > analysis after the spray with these hormones. It will be great if > anybody share there experience regarding the preparation of the > solution. I appreciate your time. > sincerely > chida > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen From kbrowning from mail.utexas.edu Wed Aug 13 09:07:06 2008 From: kbrowning from mail.utexas.edu (Karen Browning) Date: Wed Aug 13 13:25:16 2008 Subject: [Arabidopsis] methods for redox assays on plates? Message-ID: <48A2EA8A.9010504@mail.utexas.edu> Does anyone have a suite of response to redox assays (hydrogen peroxide, etc) for Arabidopsis on plates? Thank you, Karen Browning From niuyajie from wsu.edu Wed Aug 13 17:37:18 2008 From: niuyajie from wsu.edu (Niu, Yajie) Date: Fri Aug 15 12:49:52 2008 Subject: [Arabidopsis] GV3101::pMP90-RK Message-ID: Hello everybody, Does anyone have Agrobacteria strain GV3101::pMP90-RK? Unlike GV3101, this strain possess the RK2 replicase and trf gene required for the plasmid replication and I need this strain to transform with my plamids which have failed in a couple of other strains. I would greatly appreciate it if anybody could send it to me. Thanks. Yajie Niu Graduate Student Dr. John Browse Lab Institute of Biological Chemistry P.O.Box 646340 Washington State University Pullman, WA 99164-6340 From jdfriesner from ucdavis.edu Thu Aug 14 10:59:21 2008 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Fri Aug 15 12:50:01 2008 Subject: [Arabidopsis] Podcast of Somerville Lecture: Arabidopsis Conference Message-ID: <200808141559.m7EFxLgb021684@tremex.ucdavis.edu> The opening Keynote Lecture by Chris Somerville at the recent 19th International Conference on Arabidopsis Research can be accessed at this URL: https://www.ciw.edu/somerville_keynote From Madlen.Schiller from mailbox.tu-dresden.de Fri Aug 15 06:10:00 2008 From: Madlen.Schiller from mailbox.tu-dresden.de (Madlen Schiller) Date: Fri Aug 15 12:50:06 2008 Subject: [Arabidopsis] Expression of a fusionprotein Message-ID: <20080815131000.kwsv4zvo68g04s4c@mail.zih.tu-dresden.de> Hello, at the moment I try to verify a fusion protein in Arabidopsis. I have cloned the gen by using Gateway cloning (Invitrogen). I know that the DNA-sequence is right and integrated in the Arabidopsis genom. But unfortunately I get not transkripts (no RNA or Protein) in the T1-generation. Does anyone has/had also trouble with the expression of fusion proteins. I read that it is possible that the transcript will be done in further generations? Or is it possible the the Gateway cloning system decreased the expression? Thanks a lot, Madlen From rkhanna from mendelbio.com Fri Aug 15 12:42:15 2008 From: rkhanna from mendelbio.com (Rajnish Khanna) Date: Fri Aug 15 12:50:12 2008 Subject: [Arabidopsis] Research Scientist - Molecular Biology / Protein Biochemistry - Mendel Biotechnology Message-ID: <48A5BFF7.7070503@mendelbio.com> *Position Open at Mendel Biotechnology, Inc., Hayward, CA **Research Scientist - Molecular Biology / Protein Biochemistry* Mendel Biotechnology, Inc., invites applications for Research Scientist II position in Molecular Biology and Biochemistry. The successful candidate will work in the advancement of product-driven basic research with a focus on protein characterization and biochemical analysis. We seek a highly motivated scientist who can work independently and contribute to analysis of gene function in a multidisciplinary research environment. The candidate will work at the lab bench to develop and implement state-of-the-art techniques to advance knowledge of protein mode of action related to product development. The candidate will have a Ph.D. with laboratory experience in protein biochemistry, or a Masters Degree in Molecular Biology, Biochemistry, Genetics or equivalent with at least 3 years of protein based laboratory experience. Experience and knowledge of plant sciences is a plus. The candidate is expected to have excellent communication skills in English, verbal and written, and have a high level working knowledge of Microsoft computer applications. Must be a U.S. citizen or have authorization to work in the U.S. To apply, please send a letter of interest, CV, and contact information for three references (including email addresses) to: job115@mendelbio.com Visit [http://www.mendelbio.com] for more details. From jdfriesner from ucdavis.edu Fri Aug 15 14:39:50 2008 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Sun Aug 17 00:31:49 2008 Subject: [Arabidopsis] Conference books available Message-ID: <200808151939.m7FJdoiR003568@berna.ucdavis.edu> I have about 25 extra conference/abstract books from the recent Arabidopsis meeting in Montreal. If you would like one for yourself or your lab, I am willing to mail them out at just the cost of shipping/handling. The easiest way would be if you email me a FedEx number (e.g. for your lab), otherwise you'll have to send me a check or money order. Of course the conference pdf can also be accessed freely online at: http://www.arabidopsis.org/news/abstracts.jsp For more information, please email me (NOT the newsgroup!) Joanna Friesner MASC/ICAR 2008 Coordinator jdfriesner@ucdavis.edu From liq from muohio.edu Fri Aug 15 13:48:18 2008 From: liq from muohio.edu (Li, Qingshun Quinn) Date: Sun Aug 17 00:36:28 2008 Subject: [Arabidopsis] Postdoctoral position at Miami University Message-ID: A Postdoctoral Fellow position is available to study the biochemical and molecular mechanism of plant mRNA polyadenylation, and its role in gene expression regulation, plant growth and development, and responses to environmental stimuli. Funded by the US National Science Foundation, the goal of the project is to understand the functions of a set of proteins and their encoding genes in mRNA 3?-end formation. We are using two main approaches: biochemical tools to explore the roles of the proteins, and functional genomic tools to study the role of the genes. For further information about the project and recent publications, please visit http://www.polyA.org. A Ph.D. in plant biology, biochemistry, genetics, or related fields is required. Experience in molecular biology, biochemistry, and/or genetics research is essential. Knowledge of functional genomics and/or bioinformatics is desirable. The initial appointment will be for one year, and the reappointment will depend on successful performance and contingent on funding. Interested individuals should send an application package through e-mail (a cover letter detailing experience; CV; publication reprints; and names, telephone numbers and e-mail addresses of three references) to: Dr. Q. Quinn Li, Botany Department, Miami University, Oxford, Ohio 45056, USA; e-mail: liq(at)muohio.edu. Evaluation of applications will begin on August 27, 2008, until the position is filled. Miami University is a highly ranked public university located on southwest Ohio. It has state-of-the-art research facilities. The Botany Department is one of the top ranked botany departments in the USA. Miami University is an EOE/AA employer with smoke-free campuses. For information regarding campus crime and safety, visit www.muohio.edu/righttoknow. Hard copy upon request. ========================= Q. Quinn Li, Ph.D. Associate Professor Department of Botany Miami University Oxford, Ohio 45056 ======================== From zhouxin168 from gmail.com Sun Aug 17 00:05:36 2008 From: zhouxin168 from gmail.com (zhouxin168) Date: Sun Aug 17 00:36:34 2008 Subject: [Arabidopsis] Re: Expression of a fusionprotein References: Message-ID: On Aug 15, 7:10?pm, Madlen Schiller wrote: > Hello, > > at the moment I try to verify a fusion protein in Arabidopsis. I have ? > cloned the gen by using Gateway cloning (Invitrogen). I know that the ? > DNA-sequence is right and integrated in the Arabidopsis genom. But ? > unfortunately I get not transkripts (no RNA or Protein) in the ? > T1-generation. > Does anyone has/had also trouble with the expression of fusion ? > proteins. I read that it is possible that the transcript will be done ? > in further generations? Or is it possible the the Gateway cloning ? > system decreased the expression? > > Thanks a lot, > Madlen could you dectect the fusion DNA insertion in those T1-generation lines? From regis.bates from gmail.com Sun Aug 17 15:54:27 2008 From: regis.bates from gmail.com (Rb) Date: Sun Aug 17 19:14:03 2008 Subject: [Arabidopsis] Search Engine for Arabidopsis Research - Vadlo Message-ID: <94ce81ad-89ca-474b-9da9-55e526f0b38b@r66g2000hsg.googlegroups.com> Vadlo (www.vadlo.com) is great for searching techniques, databases and most of all powerpoint presentations. From solliman from yahoo.com Sun Aug 17 10:56:12 2008 From: solliman from yahoo.com (mohei solliman) Date: Sun Aug 17 19:14:30 2008 Subject: [Arabidopsis] 1st international joint conference on materials science, plant biotechnology , biotechnology, nanotechnology and health technology Message-ID: <501776.99323.qm@web35306.mail.mud.yahoo.com> Dear Professors and colleagues: It is of my pleasure to invite you to our 1st international joint conference on materials science, plant biotechnology , biotechnology, nanotechnology and health technology- MNH 09- Future Challenges which will be held in January 4- 6th, 2009 at National Research Centre (NRC), Under auspices and chairing of Prof. Dr. Hany El-Nazer, the President of NRC- Cairo, Egypt. Last year we launched our first conference on materials science and nanotechnology which got big success, so, this year we decided to widen our subjects to comprise more applicable and professional fields which are considered as the cornerstone of the modern life. Therefore, Biotechnology and complementary medicine were chosen to be our partners in that conference as promising challenging fields. All your contributions are highly encouraged, so, please do not hesitate to submit your abstract as soon as you can. All participants have to fill in the attached registration form in details identifying the major field (materials, medicine, etc.). Our conference will host eminent scientists in each field (see the attached documents). The deadline for abstract is the 1st of November. Special prices will be applied on the groups, students, young researchers and the participants of our last conference. I hope we can meet all of you at such conference to exchange science, experience and culture. With my best regards Sincerely yours Dr. MOHEI EL-DIN SOLLIMAN Plant Biotechnology Department, Division of Genetic Engineering and Biotechnology, National Research Center, El-Tahrir Str. 31, Dokki, 12622 Giza, Egypt. Fax: +2 02 333 70 931 PHONE :+2 02 37617186 lab :+2 02 39768630 home +202 38604850 home :+2 0101343324 mob. Please visit the website of Egyptian society for advanced materials and nanotechnology (www.esamnt-egypt.org)- in process Dr. MOHEI EL-DIN SOLLIMAN Plant Biotechnology Department, Division of Genetic Engineering and Biotechnology, National Research Center, El-Tahrir Str. 31, Dokki, 12622 Giza, Egypt. Fax: +2 02 333 70 931 PHONE :+2 02 37617186 lab :+2 02 39768630 home +202 38604850 home :+2 0101343324 mob. From erinc from biol.sc.edu Tue Aug 19 09:50:59 2008 From: erinc from biol.sc.edu (Erin Connolly) Date: Tue Aug 19 15:18:01 2008 Subject: [Arabidopsis] Faculty Position- University of South Carolina Message-ID: <48AADDD3.8030403@biol.sc.edu> Plant Molecular Biologist The Department of Biological Sciences at the University of South Carolina invites applications for a tenure-track position at the Assistant Professor level in the area of plant molecular biology. This position is part of a hiring initiative in the area of Plant Signaling that will strengthen existing research in Plant Biology (http://www.biol.sc.edu/~plant/PlantBiology/). The candidate is expected to establish and maintain an extramurally funded research program and to teach undergraduate and graduate courses. Candidates should submit curriculum vitae, description of research and teaching interests and three letters of reference to Dr. Erin Connolly, Plant Molecular Biology Search Committee Chair, Department of Biological Sciences, Coker Life Sciences Building, 700 Sumter St., University of South Carolina, Columbia, SC 29208 (phone 803-777-8753; email: pbsearch@biol.sc.edu, fax 803-777-4002). For full consideration, applications must be received by November 1, 2008. The University of South Carolina is an affirmative action, equal opportunity employer. Women and minorities are encouraged to apply. The University of South Carolina does not discriminate in educational or employment opportunities or decisions for qualified persons on the basis of race, color, religion, sex, national origin, age, disability, sexual orientation or veteran status. -- Erin L. Connolly Associate Professor Department of Biological Sciences University of South Carolina Columbia, SC 29208 phone: (803) 777-8753 fax: (803) 777-4002 From mlsulliv from wisc.edu Wed Aug 20 09:02:53 2008 From: mlsulliv from wisc.edu (Michael Sullivan) Date: Wed Aug 20 14:49:53 2008 Subject: [Arabidopsis] Postdoc opportunity-Madison, WI Message-ID: My lab at the US Dairy Forage Research Center in Madison, WI has =20 recently been awarded a USDA-CSREES-NRI grant to support our research =20= on the biochemical pathways responsible for o-diphenol biosynthesis =20 in red clover. This grant includes support for a postdoc position to =20 start in early 2009. Qualified candidates will have hands-on =20 experience in general molecular biology, including PCR, cloning, =20 sequencing, making gene constructs; biochemistry, including =20 characterization of proteins and analyses of enzymatic activities; =20 and/or plant transformation including plant tissue culture and =20 maintenance of transgenic plants. Candidates should also be able to =20 work independently and have good oral and written communication =20 skills. NON-US CITIZEN MAY BE ELIGIBLE FOR THIS POSITION, provided =20 they already have documentation allowing them to work in the US (I =20 CANNOT help with obtaining work visas). Non-US citizens must also =20 meet certain other eligibility requirements. Interested individuals may contact me by e-mail =20 (michael.sullivan@ars.usda.gov). The formal application process is =20 expected to begin in Dec. 2008. The US Dairy Forage Research Center is part of the Agricultural =20 Research Service of the US Department of Agriculture and is located =20 on the University of Wisconsin campus in Madison, WI. For more =20 information on the US Dairy Forage Research Center visit our web =20 site: http://ars.usda.gov/mwa/madison/dfrc Title: Elucidating the Roles of Hydroxycinnamoyl Transferases and p-=20 Coumaroyl 3-Hydroxylases in o-Diphenol Biosynthesis in Red Clover PD: Sullivan, Michael L. Institution: US Dairy Forage =20 Research Center, ARS-USDA Phenylpropanoid o-diphenols accumulate in tissues of many plants =20 functioning as defensive molecules and antioxidants. Red clover =20 accumulates high levels of two o-diphenols, phasalic acid and =20 clovamide. In red clover, post-harvest oxidation of these o-diphenols =20= to o-quinones by an endogenous polyphenol oxidase (PPO) prevents =20 breakdown of forage protein during storage. Agronomically important =20 forages like alfalfa lack both PPO and o-diphenols. Consequently, =20 breakdown of their protein upon harvest and storage results in =20 economic losses ($100 million/yr) and release of excess nitrogen into =20= the environment. Understanding how red clover is able to synthesize =20 and accumulate o-diphenols will help in development of forages that =20 take advantage of this natural system of protein protection. Also, =20 because o-diphenols are powerful antioxidants, this research has =20 implications for human and animal nutrition. Preliminary evidence =20 suggests that specific hydroxycinnamoyl transferases (HCTs) and p-=20 coumaroyl 3=92 hydroxylases (C3Hs) play key roles in red clover o-=20 diphenol biosynthesis and accumulation. Specific objectives of this =20 proposal are 1) Identify and isolate red clover gene sequences =20 encoding HCTs; 2) Characterize the HCTs and a C3H with respect to =20 substrate specificity and reaction characteristics; and 3) Establish =20 the relevance of specific HCTs to biosynthesis and accumulation of =20 specific o-diphenols. These objectives will be accomplished using =20 several complementary approaches including biochemistry, genomics, =20 and reverse genetics. --- Michael L. Sullivan Plant Research Molecular Geneticist US Dairy Forage Research Center ARS-USDA 1925 Linden Drive West Madison, WI 53706 (608) 890-0046 (Phone) (608) 890-0076 (FAX) From nebenfuehr from utk.edu Thu Aug 21 14:03:52 2008 From: nebenfuehr from utk.edu (Andreas Nebenfuehr) Date: Fri Aug 22 00:54:25 2008 Subject: [Arabidopsis] Postdoc Position in Molecular Cell Biology Message-ID: <2CE6BED8-DB36-4463-A4F5-6F7E3B068884@utk.edu> Postdoctoral Position in Molecular Cell Biology A NSF-funded postdoctoral position is available immediately to investigate protein-protein interactions involved in motor attachment to organelles in plant cells. The successful candidate will employ both affinity purification of protein complexes and yeast two-hybrid screens to identify proteins interacting with myosin motor proteins. Prior experience with these techniques is a plus but not required. This project is part of a larger effort to elucidate the molecular mechanisms underlying organelle movements and cytoplasmic streaming in plant cells. More information on our research can be found on our lab web site at http://www.bio.utk.edu/cellbiol . A Ph.D. in molecular biology, biochemistry, cell biology, or a related discipline is required. Expertise in plant molecular biology, biochemistry, and protein purification is highly desirable. Please submit a letter of interest, curriculum vitae and names of three references to Dr. Andreas Nebenf?hr University of Tennessee Department of Biochemistry, Cellular and Molecular Biology Knoxville, TN 37996-0840 USA or contact Dr. Nebenf?hr by email (nebenfuehr@utk.edu). From braam from rice.edu Fri Aug 22 20:15:38 2008 From: braam from rice.edu (Janet Braam) Date: Sat Aug 23 12:29:06 2008 Subject: [Arabidopsis] Postdoctoral Position Announcement Message-ID: <329DA997-8208-40D7-AE34-B86665E9E6D8@rice.edu> POSTDOCTORAL POSITION AVAILABLE: PLANT STRESS RESPONSES: CALCIUM SIGNALING, REDOX AND NITRIC OXIDE REGULATION, AUTOPHAGY MECHANISMS Laboratory of Janet Braam Biochemistry and Cell Biology Rice University A postdoctoral position is available in research to determine the mechanisms by which plants sense environmental stresses, interpret calcium signals, and regulate cellular and organismal responses. A Ph.D. in biological science is required. A strong background in plant biology, cell biology, biochemistry, and/or genetics is desired. Research experience in the areas of autophagy, mitochondrial function, and/or nitric oxide regulation is advantageous. Excellent communication skills and an ability and willingness to collaborate are essential. Only highly motivated individuals with proven track records should apply. Applicants should send a curriculum vitae, a cover letter describing research experience and interests, and the names and email addresses for at least three references to: braam@rice.edu Janet Braam Chair and Professor Biochemistry and Cell Biology Rice University Houston, TX 77005-1892 http://bioc.rice.edu/~braam/ Applications will be reviewed until the position is filled. Rice University is an equal opportunity/affirmative action employer. From john.woods from mail.utexas.edu Fri Aug 22 20:36:23 2008 From: john.woods from mail.utexas.edu (John O. Woods) Date: Sat Aug 23 12:31:30 2008 Subject: [Arabidopsis] Gene/loci to phenotype list, database access Message-ID: <91656c3f0808221836u32671df3x7e57323f052ae120@mail.gmail.com> Hi folks, I'm trying to obtain a list of Arabidopsis genes/loci along with a set of phenotypes resulting from knock-downs, knock-outs, and hypomorphic alleles at those loci. I would *prefer* that the phenotypes be somewhat curated. For example, FlyBase has unique identifiers for almost every phenotype, which is nice because it's easy to automatically associate two genes with the same phenotype (as opposed to by hand). I only just started looking at A. thaliana today, so I'm a bit ignorant. I've noticed TAIR has phenotypes, but I also found a disclaimer on the FTP related to just such a tab-separated database there. "Note that this only represents the data that is in the TAIR database and that there are bound to be many more loci with germplasms with phenotypes." First of all, what's a germplasm and how is it related to phenotype? Secondly, most organisms' databases don't have every last phenotype curated; is it worse with TAIR than with those other DBs, or is this disclaimer just being overly cautious? Is there a better place to get this data? I'd love to find an Arabidopsis Chado database I can either access remotely or download, or really anything I can access easily with BioPerl. Any pointers? Thanks so much for the help. Cheers, John Woods ICMB, UT Austin From rkhanna from mendelbio.com Mon Aug 25 18:49:06 2008 From: rkhanna from mendelbio.com (Rajnish Khanna) Date: Mon Aug 25 23:28:05 2008 Subject: [Arabidopsis] Research Scientist - Molecular Biology / Protein Biochemistry - Mendel Biotechnology Message-ID: <48B344F2.3020705@mendelbio.com> *Position Open at Mendel Biotechnology, Inc., Hayward, CA **Research Scientist - Molecular Biology / Protein Biochemistry* Mendel Biotechnology, Inc., invites applications for Research Scientist II position in Molecular Biology and Biochemistry. The successful candidate will work in the advancement of product-driven basic research with a focus on protein characterization and biochemical analysis. We seek a highly motivated scientist who can work independently and contribute to analysis of gene function in a multidisciplinary research environment. The candidate will work at the lab bench to develop and implement state-of-the-art techniques to advance knowledge of protein mode of action related to product development. The candidate will have a Ph.D. with laboratory experience in protein biochemistry, or a Masters Degree in Molecular Biology, Biochemistry, Genetics or equivalent with at least 3 years of protein based laboratory experience. Experience and knowledge of plant sciences is a plus. The candidate is expected to have excellent communication skills in English, verbal and written, and have a high level working knowledge of Microsoft computer applications. Must be a U.S. citizen or have authorization to work in the U.S. To apply, please send a letter of interest, CV, and contact information for three references (including email addresses) to: job115@mendelbio.com Visit [http://www.mendelbio.com] for more details. From soheil.mahmoud from ubc.ca Wed Aug 27 16:11:49 2008 From: soheil.mahmoud from ubc.ca (Mahmoud, Soheil) Date: Thu Aug 28 17:39:01 2008 Subject: [Arabidopsis] Graduate Student positions Message-ID: <6EEA43D22289484890D119821101B1DF488947@exchange20.mercury.ad.ubc.ca> Hi there: Could you please forward the following notice to all subscribers? Graduate Student Position: Two graduate student positions at the MSc level are available in the laboratory of Dr. Soheil Mahmoud at the Okanagan Campus of University of British Columbia in Kelowna, British Columbia, Canada, to study molecular basis of aroma (essential oil) formation and secretion in medicinal plants. The selected candidates will acquire training in plant tissue culture, plant molecular biology and biotechnology, and in essential oil extraction and analysis by GCMS. The student will also be involved in transcript profiling studies. Applicants should have a strong academic background in molecular biology, and an interest in natural products and medicinal plants. The candidate should possess the ability to work independently and cooperatively within a group structure. Stipend: The current NSERC rate will be given. Start Date: Enrollment in January or September 2009. However, immediate start is possible. Kelowna is home to numerous attractions including lakes, ski hills, vineries, orchards. and boasts some of the most attractive natural sceneries. Visit Kelowna at http://www.kelownabc.com/ Please contact Soheil Mahmoud at: Soheil S. Mahmoud University of British Columbia Okanagan 3333 University Way Kelowna, B.C. Canada V1V 1V7 Tel: 250.807.8752 Fax: 250.807.8005 soheil.mahmoud@ubc.ca Regards, Soheil Mahmoud Soheil S. Mahmoud Irving K. Barber School of Arts and Sciences University of British Columbia Okanagan 3333 University Way Kelowna, B.C. Canada V1V 1V7 Tel: 250.807.8752 Fax: 250.807.8005 soheil.mahmoud@ubc.ca http://people.ok.ubc.ca/smahmoud/soheilhome.htm From rgfranks from ncsu.edu Fri Aug 29 14:21:48 2008 From: rgfranks from ncsu.edu (Robert G. Franks) Date: Fri Aug 29 14:32:17 2008 Subject: [Arabidopsis] Postdoctoral Position Available Message-ID: <1DD4060A-30CF-47F6-A51B-038CBDD0B40E@ncsu.edu> Postdoctoral Position Available Genomic Analysis of Transcriptional Regulation during Carpel Margin =20= Meristem Development in Arabidopsis An NSF-funded postdoctoral position to study the development of the =20 Carpel Margin Meristem (CMM) in Arabidopsis is available in the =20 laboratory of Dr. Bob Franks in the Department of Genetics at North =20 Carolina State University, Raleigh, NC. http://www4.ncsu.edu/~rgfranks/index.html The CMM is a vital meristem that gives rise to the ovules, the =20 precursors of the seeds. The postdoctoral fellow will employ a variety =20= of molecular, genetic, and genomic approaches to clarify the functions =20= of the SEUSS, LEUNIG and AINTEGUMENTA genes in the patterning of the =20 adaxial carpel margin. See Plant Physiol. 2008 v146(3):1165-81. http://www.plantphysiol.org/cgi/content/full/146/3/1165 A Ph.D. in biology, genetics, molecular biology or equivalent is =20 required. Previous experience with Chromatin IP, whole genome =20 expression analyses and/or yeast-two-hybrid assays will be directly =20 relevant to the goals of the project. Additional experience with in =20 situ hybridization, microscopy, and molecular genetic analysis of =20 development is desirable. NCSU=92s location in the Research Triangle Area (within a half hour =20 drive of Duke University, and UNC, Chapel Hill as well as Syngenta, =20 BASF Plant Sciences, Monsanto and Bayer CropScience) engenders =20 excellent opportunities for career development and collaborative =20 research projects. The North Carolina Plant Molecular Biology =20 Consortium provides additional opportunities for multi-campus =20 interaction with a monthly seminar and annual conference. = http://www.ncbiotech.org/services_and_programs/intellectual_exchange/Plant= Bio.html APPLICATION: Applicants should submit curriculum vitae, list of =20 publications, a short statement of interests and future goals and the =20= names, phone numbers and e-mail addresses of three references to: Dr. Bob Franks 3510 Gardner Hall Campus Box7614 Department of Genetics North Carolina State University, Raleigh, NC. 27695-7614 Or by email to : rgfranks@ncsu.edu From jdfriesner from ucdavis.edu Fri Aug 29 14:58:35 2008 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Fri Aug 29 18:47:17 2008 Subject: [Arabidopsis] Arabidopsis Conference Notes and Podcast Message-ID: <200808291958.m7TJwZZF006977@phaenicia.ucdavis.edu> >From the recent 19th International Conference on Arabidopsis Research held in Montreal: (1) The keynote lecture by Chris Somerville is available as a podcast and powerpoint presentation at: http://www.ciw.edu/somerville_keynote (2) The 2009 Arabidopsis Conference will be in Edinburgh, Scotland (June 30-July 4) and the 2010 Conference will be in Yokohama, Japan (expected: June 6-10). The 2011 meeting is expected to return to Madison, Wisconsin. (3) There were requests for presentations from workshops and some were made available: Bioinformatics Workshop - http://bar.utoronto.ca/ICAR19/ TAIR Workshop- http://www.arabidopsis.org/portals/education/presentations.jsp (4) Extra conference books are still available for a limited time for just the price of shipping. Contact me (jdfriesner@ucdavis.edu) to provide a FedEx account number and have one sent to you. Joanna Friesner (for the ICAR 2008 Conference Organizers) From regis.bates from gmail.com Fri Aug 29 16:18:40 2008 From: regis.bates from gmail.com (Rb) Date: Fri Aug 29 18:52:58 2008 Subject: [Arabidopsis] Postdoc Cartoons on Vadlo Message-ID: You know, Vadlo (www.vadlo.com), biology search engine. From gleon from unab.cl Fri Aug 29 16:49:06 2008 From: gleon from unab.cl (=?iso-8859-1?Q?Gabriel_Le=F3n?=) Date: Sat Aug 30 13:27:37 2008 Subject: [Arabidopsis] Does anybody know where to buy this book? Message-ID: Hello: I want to purchase "Arabidopsis: An Atlas of Morphology and Development". However is out of print and I did not find any place were to buy it. Does anybody know where I can get it? In Amazon is unavailable, the same story in Lehle Seeds. Thanks. Gabo -- Gabriel Le?n, PhD Assistant Professor gleon@unab.cl Center of Plant Biotechnology Universidad Andr?s Bello Rep?blica 217 Santiago CHILE From altpeter from ufl.edu Sat Aug 23 10:14:39 2008 From: altpeter from ufl.edu (Altpeter,Fredy) Date: Sat Feb 7 17:12:45 2009 Subject: [Arabidopsis] (no subject) Message-ID: <18967E83B2946D4DBB46899C01D36A7336827B2F42@UFEXCH-MBXCL01.ad.ufl.edu> One Ph.D and one M.S. Graduate Assistantship in Plant Biotechnology =96 Bioenergy at the University of Florida One Ph.D and one M.S. Graduate Assistantship is available for Spring 2009 i= n the Laboratory of Plant Molecular Physiology, headed by Dr. Fredy Altpete= r (http://agronomy.ifas.ufl.edu/faculty/altpeter.shtml). Our research integ= rates advanced genetic transformation technology and functional genomics to= identify, isolate and engineer limiting factors for molecular improvement = of biomass/bioenergy grasses (http://agnetonline.com/2008/03/24/uf-receives= -1m-to-unlock-energy-from-sugarcane-residues/). Lignocellulosic materials produced by biomass grasses are a promising feeds= tock to produce low-cost ethanol, their improvement using biotechnological = approaches will reduce costs associated with biomass conversion to fuel. Ri= sk assessment and development of risk management strategies are essential c= omponents of this molecular grass improvement program. The selected candidate will focus on construction of plant expression vecto= rs for over-expression of candidate genes, generation of transgenic or muta= genized grasses, molecular, phenotypic and physiological characterization o= f transgenic grasses. The preferred candidate would have hands-on experienc= e in a range of molecular biology and/or tissue culture protocols. Send applications including CV, GRE and Toefl scores to altpeter@ufl.edu before Sept. 15th 2008. Gainesville, the home town of the University of Florida, is located half wa= y between the Atlantic Ocean and the Gulf of Mexico with a population of 16= 0 000 (48 000 students) and has frequently achieved top ratings in quality = of life and affordability http://gainesville.com/apps/pbcs.dll/article?AID= =3D/20070506/SUNFRONT/705060331&SearchID=3D73280328541268. More information on the University of Florida is available at http://www.uf= l.edu. University of Florida is an Affirmative Action/= Equal Opportunity Employer (male/female) and does not discriminate on the b= asis of race, color, creed, religion, national origin, ancestry, citizenshi= p, age, gender, sexual orientation, disability, or Vietnam Era Veteran stat= us in the offering of all benefits, services, and educational and employmen= t opportunities. Women and minorities are encouraged to apply.