From ruth from arabidopsis.info Thu Sep 4 11:28:55 2008 From: ruth from arabidopsis.info (Ruth Bastow) Date: Fri Sep 5 00:20:50 2008 Subject: [Arabidopsis] ICAR 2009 - First Announcement Message-ID: Dear All The 20th International Conference on Arabidopsis Research (ICAR) will be held in Edinburgh, Scotland, UK from June 30th to 4th July 2009. To register your interest for the conference, which will ensure you get all the latest updates and information, such as when registration and abstract submission begin, please visit https://registration.meetingmakers.co.uk/dev-cgi/ICAR_intent/begin?short_con ference_name=ICAR_intent A preliminary website for the conference is available at http://arabidopsis2009.com/ The conference will include plenary session on Systems Biology, Development, Environmental responses, Natural Variation, Plant Defence, Cell Biology and Genome Organisation. Confirmed speakers included Sally Assmann, Enrico Coen, Caroline Dean, Nicholas Harberd, Jiri Friml, Ben Scheres, and Mark Stitt. A full list of plenary sessions and chairs can be downloaded from the website http://arabidopsis2009.com/ I hope you will be able to join us in Edinburgh next year. Regards Ruth From pag from intl-pag.org Thu Sep 4 19:16:07 2008 From: pag from intl-pag.org (PAG-XVII Conference) Date: Fri Sep 5 00:20:54 2008 Subject: [Arabidopsis] CALL FOR ABSTRACTS - Plant & Animal Genome XVII Conference Message-ID: <200809050016.m850G70a000961@www.intl-pag.org> Dear Colleague: You are invited to attend the Plant & Animal Genome XVII Conference (PAG-XVII), January 10-14, 2009 taking place at the Town & Country Convention Center in San Diego, California. This is a call for abstracts and pre-registration. Everyone who submits an abstract is required to pay the registration fee prior to the meeting to be included in the Final Abstract Guide and posted on the website. You must be registered by December 1, or your abstract will NOT be printed or posted on the website. Please update us if you cannot attend so we may free up poster space in the Grand Exhibit Hall where exhibits and coffee breaks are just steps from the Atlas Foyer. Don't forget to check out the Travel Grant Applications; the deadlines are closing soon. The meeting will again offer the satellite sessions and workshops as presented in earlier conferences. A couple of new workshops this time are "Genomics of Plant Disease Resistance" and "Recombination". There will be over 100 technical, industry, and computer training workshops. The meeting draft for PAG-XVII and abstracts from PG-I through PAG-XVI are available on the PAG website at: http://intl-pag.org/ We are requesting that all abstracts be submitted electronically. Point your browser to the address below and click on the "Abstracts Submission" link when ready to submit your abstract at: http://intl-pag.org/ Due to severe delays in getting VISAs to enter the US, we recommend that you plan ahead and begin the process now. DO NOT WAIT! See the information link at the web site as updates are provided. All researchers presenting posters must pre-register for the meeting to have their abstract printed in the final meeting program. The abstract DEADLINE is Friday, October 3, 2008. Registration is available via the internet using our secure on-line registration form. Full Conference (Univ/Non-Profit/Govt) Registration for ALL attendees: Pre-Registration (deadline November 1, 2008): $550 Late Registration (after November 1, 2008): $650 On-site Registration: $750 Full Conference (Industry) Registration for ALL attendees: Pre-Registration (deadline November 1, 2008): $750 Late Registration (after November 1, 2008): $850 On-site Registration: $950 Weekend Only (Univ/Non-Profit/Govt) Registration (Fri/Sat/Sun) for ALL attendees: Pre-Registration (deadline November 1, 2008): $375 Late Registration (after November 1, 2008): $425 On-site Registration: $475 Weekend Only (Industry) Registration (Fri/Sat/Sun) for ALL attendees: Pre-Registration (deadline November 1, 2008): $475 Late Registration (after November 1, 2008): $525 On-site Registration: $575 Student Registration (proof required): Pre-Registration (deadline November 1, 2008): $350 Late Registration (after November 1, 2008): $400 All questions regarding Plant & Animal Genome XVII should be directed to Scherago Int'l at Tel: 201-653-4777 ext. 20, Fax: 201-653-5705 or Email: pag@scherago.com. Sincerely, Darrin Scherago - VP, Meeting Manager Scherago International, Inc. 525 Washington Blvd., Suite 3310, Jersy City, NJ 07310 -------------------------------------------------------------------------- Plant & Animal Genome XVII Conference ---------------- January 10-14, 2009 -------------------------------------------------------------------------- From ipmb2009 from missouri.edu Fri Sep 5 18:01:42 2008 From: ipmb2009 from missouri.edu (IPMB 2009 Conference) Date: Sun Sep 7 00:03:12 2008 Subject: [Arabidopsis] 9th IPMB Congress, St. Louis, MO, Oct 25-30, 2009 Message-ID: <200809052301.m85N1gFk025830@www.intl-pag.org> Dear Colleague: You are invited to attend the 9th International Congress of Plant Molecular Biology (IPMB), October 25-30, 2009 taking place in St. Louis, Missouri. Please look at the following website for all necessary information on this Congress which is composed of 53 symposia and 9 Plenary Lectures on all aspects of plant molecular biology. http://www.ipmb.2009.org Sincerely, Perry Gustafson - Chair IPMB 2009 -------------------------------------------------------------------------- 9th International Congress of Plant Molecular Biology, October 25-30, 2009 -------------------------------------------------------------------------- From Janet.Slovin from ARS.USDA.GOV Sun Sep 7 10:38:04 2008 From: Janet.Slovin from ARS.USDA.GOV (Slovin, Janet) Date: Sun Sep 7 15:24:28 2008 Subject: [Arabidopsis] RIN for plants Message-ID: Dear colleagues, In "Eleven golden rules of Quantitative PCR" Plant Cell 20:1736, 2008, the authors suggest checking the RNA integrity number (RIN) if using a Bioanalyzer. Last year I was told by the Agilent rep that the algorithm used to get this number was derived from animal data and would not be valid to use for plants because it fails to take into account chloroplast rRNA. Agilent appears to be willing to derive a new algorithm for plant samples, but needed input plant data from the community. So far, I haven't seen anything come out about a new algorithm from Agilent, so I'm wondering what other people are using. I've been manually examining my ribosomal peak shapes and peak ratios, checking for DNA, and just going with the concentrations the program gives me for each sample. I would greatly appreciate some guidance. Thanks, Janet Janet Slovin, Ph.D. Genetic Improvement of Fruit and Vegetables Laboratory USDA/ARS 10300 Baltimore Ave Beltsville, MD 20705-2350 Tel: 301-504-5629 FAX: 301-504-5653 Please note new laboratory name and new email address janet.slovin@ars.usda.gov From zepedrof from gmail.com Mon Sep 8 11:04:36 2008 From: zepedrof from gmail.com (Jose Pedro Fonseca) Date: Mon Sep 8 19:30:51 2008 Subject: [Arabidopsis] Gateway compatible, reporter GFP destination vector needed Message-ID: <774498f60809080904t38732646tf8b1f2a25794e2c1@mail.gmail.com> Dear all, We have cloned quite a few (high-throughput) sugar cane genes of interest in gateway entry vector (BP) but we had problems in the LR reactions with our binary destination vectors. Now we are looking for a new, reliable Gateway-compatible GFP reporter destination vector. Could someone recomend or kindly provide such a vector to us please? Our goal is to observe the sub celular localization of dozens/hundreds of sugar cane proteins through biobalistics in onion epidermal cells. Many thanks, JP fonseca From mmsevans from stanford.edu Mon Sep 8 11:13:43 2008 From: mmsevans from stanford.edu (Matt Evans) Date: Mon Sep 8 19:30:59 2008 Subject: [Arabidopsis] Postdoctoral Position Available in Maize Gametophyte Development Message-ID: Postdoctoral Position Available in Maize Gametophyte Development FUNCTIONAL GENOMICS OF MAIZE GAMETOPHYTES Laboratory of Matt Evans Carnegie Institution for Science Department of Plant Biology Stanford, CA 94305 A postdoctoral position is available starting immediately to study the functional genomics of maize gametophyte development. The successful applicant will join an NSF Plant Genome Research Program-funded collaboration between the Carnegie Institution, Oregon State University, Iowa State University, South Dakota State University, and Stanford University. The project uses molecular, genetic, and bioinformatic tools to identify genes required for gametophyte development and function in maize. A Ph.D. in molecular biology, genetics, or a related field is required. A strong background in genetics and molecular biology is desired. Experience in maize genetics is advantageous but not required. Excellent communication skills and an ability and willingness to collaborate are essential. The successful applicant is also expected to interact with undergraduates participating in the project through the Stanford Science in Service Program. Interested individuals should send an application package through e-mail (a cover letter detailing experience; CV; publication reprints; and names, telephone numbers and e-mail addresses of three references) to Matt Evans, Carnegie Institution for Science, Department of Plant Biology, 260 Panama St., Stanford, CA 94305 USA; phone: 650-325-1521 x.283; fax: 650-325-6857; email: mmsevans@stanford.edu The Carnegie Institution is an equal opportunity employer. -- Matt Evans Carnegie Institution for Science Department of Plant Biology 260 Panama St. Stanford, CA 94305 phone: (650) 325-1521 ext.283 fax: (650) 325-6857 From sanjiban.c from gmail.com Tue Sep 9 09:19:15 2008 From: sanjiban.c from gmail.com (sanjiban chakrabarty) Date: Tue Sep 9 14:48:36 2008 Subject: [Arabidopsis] BAC Library construction Message-ID: <8403d0360809090719x786d1749yd2c2fcb00706cbba@mail.gmail.com> Dear Sir, I am a PhD student working in India . I have been attempting to construct a High Molecular Weight (HMW) DNA library using BAC but have been completely unsuccessful at producing clones. I know the problem is with ligating the HMW DNA to the vector. I have already checked the ligase and my vector and neither of those appears to be the problem. The problem seems to be restricted to the HMW DNA.I am also worried about the ligation reaction as I have already tried with different molar ratios as advised in many papers but couldn't get any better result. Can you please help me in this regard. Your help would be greatly appreciated. Waiting for your kind reply, Regards, Sanjiban From lbp8 from cornell.edu Tue Sep 9 14:27:00 2008 From: lbp8 from cornell.edu (Lucy Pola) Date: Tue Sep 9 14:48:44 2008 Subject: [Arabidopsis] job posting Message-ID: <48C6CE04.4020903@cornell.edu> Please post this job to the list serve. Thank you. If you have any questions, please let me know. Lucy *Postdoctoral Positions to Characterize Salicylic Acid Signaling Network in Plants at Cornell Univ./BTI* The plant hormone salicylic acid (SA) affects many diverse processes in plant growth and development and plays important role(s) in response to abiotic and biotic stresses. Its role(s) in disease resistance has been the most extensively studied but is still only partially understood (e.g. PNAS 2005, 102:1773; Science 2007, 318:113; Curr. Opin. Plant Biol. 2008, 11:436). To obtain insights into how SA carries out its varied functions, a large number of putative SA-binding proteins (pSABPs) have recently been identified in Arabidopsis by affinity chromatography. Investigators will use two powerful high throughput screens, an Arabidopsis protein micro-array and a yeast three hybrid system, to identify additional SABPs, as well as confirm the SA-binding activity of the already identified pSABPs. These SABPs will be characterized using biochemical, genetic, molecular, bioinformatic, and biophysical (X-ray crystallography) techniques to decipher their functions in growth and development and/or responses to abiotic or biotic stresses (particularly disease resistance), and the effects of SA binding on these functions. Based on current data and the results obtained, an SA signaling network model(s) will be constructed, tested by more detailed characterization of putative signaling or regulatory modules, and refined in order to produce an exemplary model of the SA signaling network of SABPs which may serve as a paradigm for other plant hormones (and other small ligands). This multi-disciplinary project will be supported by a 4-year NSF 2010 grant. Applicant should have a Ph.D. in biochemistry, molecular biology, chemistry, genetics, or related areas and must be strongly motivated and dedicated to doing basic research. */Strong background in protein chemistry and enzymology is preferred./* */ /* Send CV and a cover letter detailing experience and _have_ three letters of recommendation sent to: Dan Klessig, Boyce Thompson Institute for Plant Research at Cornell University, Tower Road, Ithaca, NY 14853 or email dfk8@cornell.edu . Related website http://bti.cornell.edu/DanKlessig.php Institute Description: BTI is a not-for-profit research institute affiliated with Cornell University. Its research emphasis is on fundamentally understanding the basic biology of plants, particularly as this relates to signal transduction within a plant and between a plant and its environment. BTI is an Equal Opportunity/Affirmative Action Employer. -- Lucy Pola, SPHR Director of Human Resources Boyce Thompson Institute Tower Road Ithaca, NY 14853 607-254-1239 lbp8@cornell.edu http://bti.cornell.edu From rupesh.tayade from gmail.com Wed Sep 10 01:43:37 2008 From: rupesh.tayade from gmail.com (rupesh tayade) Date: Wed Sep 10 16:46:34 2008 Subject: [Arabidopsis] problem in pcr Message-ID: <4d88be80809092343q7993156egf60892f01ef6bdf8@mail.gmail.com> i am working on tomato disease resistant gene screening i.e MAS( marker assisted selection) . i have did the DNA isolation from green house plant and open field plant. and complete the pcr for diffent resistant gene. for near about 1500 sample the pcr is working fine got the good amplification, but when i have again collected the samples after 30 day the DNA Quality looks good on gel ,but not get amplified in pcr reaction. we are tried the things but its not working. i would be thankful if some one suggest me to over come the problem. lookinforward for suggestion With Regards Rupesh From cdtown from jcvi.org Thu Sep 11 04:43:55 2008 From: cdtown from jcvi.org (Town, Christopher D.) Date: Fri Sep 12 01:33:51 2008 Subject: [Arabidopsis] Bioinformatics Analyst Position Message-ID: <9F9FBD4CF09A1446BE3EDFA1F3A49E87024C4B78@EXCHANGE.TIGR.ORG> I realize that Medicago is not Arabidopsis, but it is another model organism. You might be interested in this. Bioinformatics Analyst Requisition Code: PLAN688 Location: Rockville, Maryland Department: Plant Genomics Schedule: Full Time Job Responsibilities: The Bioinformatics Analyst will work on the curation and annotation of the M. truncatula genome sequence. Responsibilities will include design and maintenance of web pages providing access to Medicago genome sequence and annotation and associated data, including outreach to the users through a community annotation portal. The ideal candidate will have hands-on research experience in Medicago genomics and demonstrated competence in bioinformatics including Perl, SQL, HTML and cgi-scripting. Qualifications: Successful candidates will have a Bachelor's or Master's degree in the Biological Sciences, plus experience working with computers in the field of biological sciences and a familiarity with DNA sequence and annotation tools and practices. Candidates will also have a demonstrated competence in appropriate computer languages including Perl, SQL, HTML and cgi-scripting; web technologies including HTML, DHTML, XML, CSS; Graphics tools including Photoshop, Fireworks or similar; Operating Systems including UNIX and UNIX variants, Windows, and/or Macintosh; Relational Database including knowledge of Sybase and SQL. The Bioinformatics Analyst will have a solid knowledge of molecular biology as it relates to DNA sequences, sequencing technologies and genomics. Strong written and verbal communication skills, excellent organization and time management skills, ability to meet schedules, the ability to collaborate across a multi-disciplinary team, and work independently is a must. You can apply through the JCVI web site; the position is listed under Plant Genomics. https://careers.jcvi.org/careers/ ___________________________ Chris Town Investigator The J. Craig Venter Institute 9704 Medical Center Drive Rockville, MD 20850 Office Phone: 301-795-7523 Fax: 301-838-0208 Home Phone: 301-990-0878 Cell Phone: 301-204-6300 From ipmb2009 from missouri.edu Thu Sep 11 13:41:03 2008 From: ipmb2009 from missouri.edu (IPMB 2009 Conference) Date: Fri Sep 12 01:34:23 2008 Subject: [Arabidopsis] 9th IPMB Congress, St. Louis, MO, Oct 25-30, 2009 Message-ID: <200809111841.m8BIf3dE029848@www.intl-pag.org> Dear Colleague: You are invited to attend the 9th International Congress of Plant Molecular Biology (IPMB), October 25-30, 2009 taking place in St. Louis, Missouri. Please look at the following website for all necessary information on this Congress which is composed of 53 symposia and 9 Plenary Lectures on all aspects of plant molecular biology. http://www.ipmb2009.org Sincerely, Perry Gustafson - Chair IPMB 2009 -------------------------------------------------------------------------- 9th International Congress of Plant Molecular Biology, October 25-30, 2009 -------------------------------------------------------------------------- [Apologies, the URL sent from a previous message has been corrected here.] From jdfriesner from ucdavis.edu Thu Sep 11 19:36:02 2008 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Fri Sep 12 01:37:04 2008 Subject: [Arabidopsis] Arabidopsis Conference Survey Summary Message-ID: <200809120036.m8C0a2QI021587@tremex.ucdavis.edu> If you are interested in seeing a summary of responses to the Arabidopsis conference survey please see: http://www.arabidopsis.org/portals/masc/journal.jsp#survey08 From xwwang from uga.edu Thu Sep 11 10:12:56 2008 From: xwwang from uga.edu (xwwang@uga.edu) Date: Fri Sep 12 01:37:38 2008 Subject: [Arabidopsis] methods for rescue T-DNA in transformants Message-ID: <20080911111256.ODW47379@punts3.cc.uga.edu> Dear all, I am trying to rescue the T-DNA insertion and try to know the sequence which is flanking the T-DNA left or right border in the plant transformants. The transformed plants have a big genome. Now I try to find a very useful method to know the insertion site in the plant genome. Do you know any methods or have any experiences or suggestions in how to rescue / recover the T-DNA insertion? Could you let me know? please email me: xwwang@uga.edu Thanks xuewen From yw195203 from ohio.edu Thu Sep 11 14:24:26 2008 From: yw195203 from ohio.edu (yw195203@ohio.edu) Date: Sat Sep 13 12:36:10 2008 Subject: [Arabidopsis] Gel doc with SYBR-safe/Blue-Light --- recommended camera? Message-ID: <1221161066.48c9706aafe14@webmail.ohio.edu> Our lab is making the conversion to gel documentation using SYBR-Safe stain and a blue-light transilluminator. We have a Safe Imager illuminator (S37102) with a 20x20 cm viewing surface, and plan to get a Spectroline darkroom hood (GL-1001) with 58mm camera mount. We need to find a good digital camera with swivel LCD. Any recommendations? We would also like advise regarding image printing. (e.g., inexpensive thermal printer, alternatives to thermal printing, etc.). Thanks! Jimmy Triplett Yunjing Wang Kellogg Lab, UMSL From bouchez from versailles.inra.fr Wed Sep 17 08:06:29 2008 From: bouchez from versailles.inra.fr (David Bouchez) Date: Wed Sep 17 23:37:39 2008 Subject: [Arabidopsis] Post-doc position in Cell Biology at IJPB (Versailles, France) Message-ID: <58FB4DE9-EB57-4BAA-8A7E-59DDF4173A57@versailles.inra.fr> POST-DOCTORAL POSITION IJPB, Versailles, France Recent results obtained in our group on the characterization of the TON1, FASS/TON2 proteins and their partners uncovered converging evidences pointing towards evolutionary conservation of some centrosomal components at the plant cell's cortex. TON1 and FASS themselves are related to proteins recently shown to localize at the centrosome in animal cells. TON1 interacts with centrin, a key component of eucaryotic MTOCs. Moreover, several TON1 and FASS interactants belong to a new family of cytoskeleton-associated proteins called TIM which are reminiscent of some animal centrosomal proteins. These are the first indications ever for a functional and evolutionary link between animal centrosomes and the cortical cytoskeleton of plants, apart from gamma-tubulin dependent nucleation. Through a collaboration between our group and the group of Marilyn Vantard the successful candidate will work on the functional characterization of TON1 and TIM proteins in plant cells, and of their other interacting partners in the cell from a cytological, molecular genetics and biochemical point of view. The position is available for candidates with outstanding academic record and excellent experimental and communication skills. It is located in Versailles (France) for 30 months, starting January 2009. Part of the work will be done in Grenoble, in Marilyn Vantard's group. Salary is 2200-2500 euros per month. Requirements : Candidates must have a PhD with an excellent academic record. We are seeking individuals with a strong background in genetics, molecular biology, biochemistry and microscopy. Experience in protein biochemistry and/or with fluorescent protein detection and confocal laser scanning microscopy is a plus. Experience in plant biology and knowledge of the French language are not mandatory. Application : Please send cover letter, CV, publication reprints and names and e-mail addresses of three references to David Bouchez (bouchez@versailles.inra.fr ). The successful candidate will start in January 2009 or soon after. Relevant publications : Azimzadeh et al (2008). Arabidopsis TONNEAU1 proteins are essential for preprophase band formation and interact with centrin. Plant Cell, in press Pastuglia & Bouchez (2007). Molecular encounters at microtubule ends in the plant cell cortex. Curr Op Plant Biol 10:1-7 Pastuglia et al (2006). Gamma-tubulin is essential for microtubule organization and development in Arabidopsis. Plant Cell 18 : 1412-1425 Camilleri et al (2002). The Arabidopsis TONNEAU2 gene encodes a putative novel PP2A regulatory subunit essential for the control of cortical cytoskeleton. Plant Cell, 14 : 833-845. The Jean-Pierre Bourgin Institute hosts 25 research groups devoted to plant biology, totalizing 330 people, comprised of 230 permanent staff and 100 post-docs, PhD students and others students. The IJPB has a long-standing and respected expertise in the field of plant biology, including genomics, genetics, breeding, metabolism, cell biology and development. All tools and resources relevant for integrative plant biology are available on site. Local facilities in Versailles include outstanding experimental facilities dedicated to plant culture (4500 m2 glasshouses and 500 m2 controlled environment rooms), state of the art technical platforms for Microscopy and Cytology (4 confocal microscopes, classical microscopy, etc), Protein Biochemistry (electrophoresis, chromatography, MALDI-TOF MS…), Chemistry (FT-IR, several mass spec including GC-TOF and LC-MS/MS…), and Bioinformatics. Further details and information : bouchez@versailles.inra.fr David Bouchez Institut Jean-Pierre Bourgin Station de Génétique et Amélioration des Plantes INRA Centre de Versailles - 78026 Versailles Cedex France tel : +33 (0)1 30 83 33 94 - fax : +33 (0)1 30 83 33 19 e-mail : bouchez@versailles.inra.fr http://www-ijpb.versailles.inra.fr cytoskeleton group : http://www-ijpb.versailles.inra.fr/en/sgap/equipes/cyto/ From mohan from unimelb.edu.au Sun Sep 21 08:57:50 2008 From: mohan from unimelb.edu.au (Mohan Singh) Date: Sun Sep 21 12:02:19 2008 Subject: [Arabidopsis] Post Doctoral Positions in Plant Molecular Biology Message-ID: POST DOCTORAL POSITIONS IN PLANT MOLECULAR BIOLOGY IN THE UNIVERSITY OF MELBOURNE AUSTRALIA Expressions of interest are sought to fill postdoctoral positions within the Plant Molecular Biology and Biotechnology group, at the Melbourne School of Land and Environment at The University of Melbourne, highest ranked Research University in Australia. We are interested in recruiting highly motivated individuals with a desire to succeed and contribute to the internationally competitive research activities of the laboratory focussing on molecular mechanisms of differentiation and cell specific gene regulation during meristem development, floral transition and reproductive differentiation. A brief description of research projects is provided below. MicroRNAs and Epigenetic Regulation of Meristem Development MicroRNAs (miRNAs) are small non-coding RNAs that regulate expression of many genes. We have recently discovered a network of shoot apical meristem specific miRNAs. These include novel plant miRNAs being reported for the first time. The postdoctoral research project will involve investigation of role of these novel miRNAs in meristem development and floral transition using computational and experimental tools. The experimental approach will include expression profiling of individual miRNAs through gel blots, microarrays, in situ localizations combined with gain-and loss-of-function studies in transgenic plants. Molecular Control of Stem Cell Maintenance in Plants Shoot apical meristems (SAMs) provide niches for self-sustaining groups of stem cells that play a critical role in plant development being founder cells of all aerial plant parts. Maintenance of these stem cells is thus essential for repetitive organ initiation throughout plant lifecycle. Using high throughput gene expression profiling technologies we have identified subset of novel genes that are differentially expressed in shoot apical meristem of pea and soybeans. The postdoctoral appointee will participate in a project addressing developmental role of these genes. The approaches will include laser capture microdissection, mutant analyses, in situ hybridisations, and reporter gene expression and gain-and loss-of-function studies in transgenic plants. Male Germ Line Development and Fertilization in Flowering Plants Unlike animals where germ line cells diverge from somatic cells during early embryo development the plant germ lineages initiate late in development at flowering stage. The male germline in plants begins with an asymmetric division, after which one of the resulting cells, the generative cell, divides symmetrically to produce two sperm cells. Research from our laboratory has revealed that the male germ line cells show transcriptional repertoire that is highly divergent from other reproductive and somatic cells. The post-doctoral appointee will participate in a project focusing on role of cell specific genes and nature of associated regulatory mechanisms. Potential approaches include Potential approaches include DNA-protein interaction analyses, chromatin immunoprecipitation (ChiP), gene knockout in transgenic plants, proteome and methylome analysis, live imaging of fertilization events. Applicants should possess a PhD, together with relevant experience in one or more fields of functional genomics, molecular genetics and cell biology and be able to provide evidence of proven research productivity in the form of publications in refereed journals. Candidates should also be experienced in general molecular biology techniques and ideally have expertise in one or more of following techniques/approaches; protein-DNA interactions, protein-protein interactions, ChIP, protein biochemistry and bioinformatics. The positions are being offered at Research Fellow ( Level A) position with salary range of $63,451-$68,109 p.a. Candidates with exceptional achievements can be considered for appointment at a higher salary level. Applications close on 17 th October 2008. For further details contact: Professor Prem L Bhalla, tel. +61 3 8344 9651, email premlb@unimelb.edu.au or Professor Mohan B Singh, tel. +61 3 8344 5051, email mohan@unimelb.edu.au From danielle.marcos from gmail.com Mon Sep 22 16:39:31 2008 From: danielle.marcos from gmail.com (Danielle M) Date: Tue Sep 23 02:09:28 2008 Subject: [Arabidopsis] Sourcing FM4-64 Message-ID: Hello! I am looking into buying FM4-64 to stain cell membranes in living Arabidopsis shoots. I see that i can buy 1 mg of FM4-64 from Invitrogen/Molecular Probes for about $400 (CAD.) I've also found a second supplier, Calbiochem, that sells FM4-64 under the name SynaptoRed for $240 (CAD) for 5 mg. The publications i've looked at have all used FM4-64 from Molecular Probes. I'm wondering if anyone has used Calbiochem's SynaptoRed/FM4-64 in Arabidopsis, and if they could comment on it's efficacy at staining cell membranes. Thanks very much, danielle From phoebe from deakin.edu.au Tue Sep 23 17:30:40 2008 From: phoebe from deakin.edu.au (Phoebe Chen) Date: Wed Sep 24 11:21:51 2008 Subject: [Arabidopsis] APBC2009 Poster Extended to 26 Sep Message-ID: <7.0.1.0.2.20080923214718.05360190@deakin.edu.au> Dear Colleagues, Due to numerous requests, APBC2009 Poster Submission has been extended to 26, Sep, 2008. We apologize if you receive multiple copies of this call for posters extension. Regards, Organizing Committee of APBC2009 ------------ CALL FOR POSTERS (APBC 2009) The Seventh Asia-Pacific Bioinformatics Conference (APBC2009) Beijing, China, 13-16 January 2009 http://bioinfo.au.tsinghua.edu.cn/apbc2009/ Please consider to submit a poster in the conference. Due to numerous requests, the deadline for Posters submission is extended to this Friday, 26 Sept, 2008. The details of the call for posters can be found here: http://www.easychair.org/conferences/?conf=apbc2009poster. We are looking forward to meeting you in Beijing., an exciting place to explore. Organizing Committee of APBC2009 APBC2009 PC co-chairs Michael S. Waterman Michael Q. Zhang Xuegong Zhang From hugo.zheng from mcgill.ca Wed Sep 24 16:15:17 2008 From: hugo.zheng from mcgill.ca (Hugo Zheng) Date: Wed Sep 24 18:46:31 2008 Subject: [Arabidopsis] Sourcing FM4-64 Message-ID: <000001c91e8a$a4da7470$c9c4ce84@MCGILLCEC1FBBD> If anyone has experience with SynaptoRed C2 in endocytosis in plant cells, please comment here. It is much cheaper than Invitrogen. Hugo ---------------------------------------------------- Hugo Zheng, PhD Department of Biology, McGill University 1205 Dr Penfield Avenue Montreal, QC, H3A 1B1 phone: 1 514 398-1328 fax: 1 514 398-5069 http: www.biology.mcgill.ca/faculty/zheng From FVigneault from nrcan.gc.ca Wed Sep 24 19:38:19 2008 From: FVigneault from nrcan.gc.ca (=?iso-8859-1?Q?Vigneault=2C_Fr=E9d=E9ric?=) Date: Thu Sep 25 01:04:35 2008 Subject: [Arabidopsis] Sourcing FM4-64 References: <000001c91e8a$a4da7470$c9c4ce84@MCGILLCEC1FBBD> Message-ID: <8C16DEDEF6DE8B418A724B298297A53013A291@S0-OTT-X3.nrn.nrcan.gc.ca> Seriously, you should 1st ask the Sales Rep if it's comparable, if he says yes, then you buy it and try it. If it doesn't work as you want you call the Rep back and you ask him why or to get reimbursed, then you go at Invitrogen. In fact if you look for the CAS or molecules. They both are invariably used in vesicle trafficking and endocytosis studies. A simple google search led me to a fair amount of paper and pubmed links... I hope it helps to -----Original Message----- From: arab-gen-bounces@oat.bio.indiana.edu on behalf of Hugo Zheng Sent: Wed 9/24/2008 5:15 PM To: arab-gen@magpie.bio.indiana.edu Subject: [Arabidopsis] Sourcing FM4-64 If anyone has experience with SynaptoRed C2 in endocytosis in plant cells, please comment here. It is much cheaper than Invitrogen. Hugo ---------------------------------------------------- Hugo Zheng, PhD Department of Biology, McGill University 1205 Dr Penfield Avenue Montreal, QC, H3A 1B1 phone: 1 514 398-1328 fax: 1 514 398-5069 http: www.biology.mcgill.ca/faculty/zheng _______________________________________________ Arab-gen mailing list Arab-gen@net.bio.net http://www.bio.net/biomail/listinfo/arab-gen From zhao.124 from osu.edu Thu Sep 25 12:14:49 2008 From: zhao.124 from osu.edu (QIAO ZHAO) Date: Thu Sep 25 18:12:38 2008 Subject: [Arabidopsis] mobile small RNA ? In-Reply-To: <000001c91e8a$a4da7470$c9c4ce84@MCGILLCEC1FBBD> References: <000001c91e8a$a4da7470$c9c4ce84@MCGILLCEC1FBBD> Message-ID: Hi All, Does anyone know if small RNA from RNAi or antisense can travel from tissue to tissue in plants? I would like to know if tissue-specific down regulate a gene would make any sense. thank you Qiao Zhao The Ohio State University Department of Plant Cellular and Molecular Biology 244 Rightmire Hall laboratory 1060 Carmack Road Columbus, OH 43210 Tel:(614)-292-5623 From cl_shi from hotmail.com Fri Sep 26 02:24:27 2008 From: cl_shi from hotmail.com (ShiCL) Date: Fri Sep 26 11:26:41 2008 Subject: [Arabidopsis] mobile small RNA ? In-Reply-To: References: <000001c91e8a$a4da7470$c9c4ce84@MCGILLCEC1FBBD> Message-ID: Sure, dsRNA induced silencing was shown to be able to move from tissue to tissue in plant, but amiRNA was specific. Check with systemic RNA silencing or amiRNA Weigel, you will find the related papers. Best wishes! Sincerely yours, Chunlin Shi Department of Molecular BiosciencesPO box 1041 BlindernN-0316 OsloNorway> From: zhao.124@osu.edu> To: arab-gen@magpie.bio.indiana.edu> Date: Thu, 25 Sep 2008 12:14:49 -0500> CC: > Subject: [Arabidopsis] mobile small RNA ?> > > Hi All,> > Does anyone know if small RNA from RNAi or antisense can travel from> tissue to tissue in plants? I would like to know if tissue-specific> down regulate a gene would make any sense.> > thank you> Qiao Zhao> The Ohio State University> Department of Plant Cellular and Molecular Biology> 244 Rightmire Hall laboratory> 1060 Carmack Road> Columbus, OH 43210> Tel:(614)-292-5623 From wullschlegsd from ornl.gov Fri Sep 26 16:15:38 2008 From: wullschlegsd from ornl.gov (Wullschleger, Stan D.) Date: Fri Sep 26 17:59:53 2008 Subject: [Arabidopsis] Ecotox and Microarrays Message-ID: All, I am looking for gene expression studies that show the response of Arabidopsis to either heavy metals, or inorganic/organic pollutants. I would be especially interested in accessing data in which a single study compared patterns of gene expression for two or (preferably) more heavy metals and/or toxicants. Thanks, -------------------------------------------- Stan Wullschleger, PhD Environmental Sciences Division Oak Ridge National Laboratory Oak Ridge, TN 37831-6422 Tel (865) 574-7839 Fax (865) 576-9939 Email: wullschlegsd@ornl.gov -------------------------------------------- From zhan0655 from umn.edu Fri Sep 26 23:00:34 2008 From: zhan0655 from umn.edu (Feng Zhang) Date: Mon Sep 29 12:03:23 2008 Subject: [Arabidopsis] dominant negative selection marker Message-ID: <48DDAFE2.5050404@umn.edu> Hi All, I am trying to make a dominant selection system in arabidopsis. Can anyone recommend a clean and robust marker gene? Dao1 gene seems like a good candidate. Does anyone know where I can get this gene? Any input would be highly appreciated. Thanks From incle from psb.ugent.be Tue Sep 30 04:13:12 2008 From: incle from psb.ugent.be (Inge De Clercq) Date: Tue Sep 30 13:29:16 2008 Subject: [Arabidopsis] forum question about yeast-one-hybrid Message-ID: <48E1EDA8.6070801@psb.ugent.be> subject: _yeast-one-hybrid beta-galactosidase assay _Dear all, I am performing a yeast one hybrid screen according the protocol of Deplancke with histidin and beta-galactosidase selection. After screening with a cDNA library, I picked up some colonies on medium lacking histidin + a concentration of 3-AT at which the strain does not self-activate. I retested these positives on a new plate with the same composition: a few colonies could not grow (which seems to me a negative control that is ok), but others again growed well, even a higher concentration of 3-AT... So, it seems to me that there s nothing wrong with this first test. The problem is that all these colonies don't give blue color after performing the beta-gal assay (protocol according to deplancke, 2006 and clontech: colony-lift filter assay). I tried already multiple times. Does someone have an idea what could be wrong? The construct containing the LacZ gene is ok: confirmed with PCR, and some strains were blue during testing the self activation in the beginning. Could it be that maybe my not-self activating strain (that i selected for performing the screen) is contaminated with neighboring colonies at my master plate that were self activating, and that the colonies that i picked up after my screen are just self activating colonies instead of positive transformants? Many thanks in advance... greetings, Inge From sanjaysingh765 from gmail.com Tue Sep 30 01:16:05 2008 From: sanjaysingh765 from gmail.com (chunnu) Date: Tue Sep 30 13:29:54 2008 Subject: [Arabidopsis] highly expressed gene Message-ID: <06a71487-a9a0-480a-8563-5ad395de1b8e@f63g2000hsf.googlegroups.com> I am a graduate student. I am looking for the highly and lowly expressed genes of rice and Arabidopsis gene ( say 10 % of whole genomes). I know that there is a database called MPSS for this purpose but due to lack of my knowledge I am not able to extract out the data of my interest from this database. So please help me in this regards.You can mail me at sanjaysingh765@gmail.com also. Best regards Sanjay