From me.talsherman from gmail.com Wed Apr 1 08:07:19 2009 From: me.talsherman from gmail.com (Tal Sherman) Date: Wed Apr 1 17:57:36 2009 Subject: [Arabidopsis] Fulbright Israel Post-Doctoral Fellowships for American Researchers in All Academic Disciplines Message-ID: *Fulbright Israel Post-Doctoral Fellowships for* *American Researchers in All Academic Disciplines* The United States-Israel Educational Foundation (USIEF), the Fulbright commission for Israel, offers 10 fellowships to American post-doctoral researchers in support of work to be carried out at Israeli universities during the course of the 2010/2011-2011/2012 academic years. The US Post-Doctoral Fellowship Program is open to candidates in all academic disciplines. Program grants total $40,000, $20,000 per academic year. Program fellows must be accepted as post-doctoral researchers by Israeli host institutions, which agree to provide them with a standard post-doctoral grant, which they will receive in addition to their Fulbright Fellowship. Applications for 2010/2011-2011/2012 Fulbright Post-Doctoral Fellowships must be submitted to the Council for International Exchange of Scholars by August 1, 2009. Further details on the program and on application procedures may be found at: http://www.fulbright.org.il/index.php?id=1317; http://www.cies.org/award_book/award2010/award/Pos0441.htm; http://www.cies.org/us_scholars/us_awards/Application.htm. Inquiries may be directed to Ms. Judy Stavsky, Deputy Director, USIEF, at jstavsky@fulbright.org.il or +972-3-517-2392. From xxx from xxx.com Wed Apr 1 11:21:54 2009 From: xxx from xxx.com (Stephan Wenkel) Date: Wed Apr 1 17:58:07 2009 Subject: [Arabidopsis] PhD position University of Tuebingen/Germany Message-ID: A PhD-student position is available in the laboratory of Stephan Wenkel (Center for Plant Molecular Biology (ZMBP), University of T?bingen/Germany) starting June/July 2009. The laboratory studies the formation of leaves in the model plant Arabidopsis thaliana using molecular biological and biochemical approaches. Requirements: Diploma/Masters degree in Biology (preferably developmental biology or molecular genetics); experience in molecular genetics lab techniques. Ability to work in a team and good knowledge of English are required. Applications with Curriculum vitae and a motivation letter should be sent to Stephan Wenkel (wenkel [at] stanford [dot] edu). In addition, please arrange for one or two letters of reference to be sent by e-mail as well. From benholt from ou.edu Fri Apr 3 10:10:05 2009 From: benholt from ou.edu (Holt, Ben F. III) Date: Fri Apr 3 19:00:08 2009 Subject: [Arabidopsis] Semi Solid Agarose Library Amplification Message-ID: Hi Everyone, Last week I requested protocols for semi-solid agarose amplification of cDNA libraries. Scott Michaels and Jean Danyluk were both nice enough to dig out the company manuals with those protocols (and both even directed me to the page number!). Several others wrote to me asking for the same information, so I cut the pages from those Gibco and Stratagene manuals and created a single PDF document - it's attached. Enjoy and thanks again Jean and Scott. Ben ======== Ben Holt Assistant Professor University of Oklahoma Department of Botany and Microbiology GLCH Rm 219 770 Van Vleet Oval Norman, OK 73019 Phone (405)325-9018 FAX (405)325-7619 http://www.ou.edu/cas/botany-micro/faculty/holt.html From benholt from ou.edu Sat Apr 4 18:00:48 2009 From: benholt from ou.edu (Holt, Ben F. III) Date: Mon Apr 6 21:16:14 2009 Subject: [Arabidopsis] Semi Solid Attempt 2 Message-ID: Hi everyone, Apparently there were problems with the last attachment - I went back and checked the message I sent and the attachment was there, so I conclude that the listserv is either blocking these in general or just didn't like the one I sent. Anyway, I have attached it here again and I have pasted the two protocols below - sorry, but the pasted protocols below are not very pretty. I had to choose between making them look nice or watching the Final Four. Go Heels! Ben Additional Protocols Note: Carbenicillin reduces satellite formation. Caution: Make sure that the rotor was set at room temperature for at least 2 h before adding the bottles. Rotors at 4?C will cause solidification of agar. 5.1 Expansion of Plasmid cDNA Libraries Semi-solid amplification of primary cDNA transformants minimizes representational biases that can occur during the expansion of plasmid cDNA libraries (25). Life Technologies has further modified this protocol such that the amplification is done at 30?C, helping to stabilize unstable clones (26). Caution: Bottles of semi-solid agar containing suspended colonies must be handled gently and incubated without disturbance. Rough handling or bumping of the incubator will cause micro colonies to fall out of solution. Incubators that have fans can cause colonies to fall out of solution. 1. Prepare 2.0 L of 2X LB. Remove 175 ml of the 2X LB to make the 2X LB Glycerol. 2. Place a large stir bar and 1.35 g SeaPrep? agarose into each of four 500-ml autoclavable bottles. Place bottles on stir plates. With the stir plate turned on, add 450 ml of 2X LB to each bottle (avoid the formation of large clumps of agarose). 3. Autoclave these bottles of 2X LB agarose for 30 min. 4. Cool bottles in 37?C water bath for ~2 h until the medium reaches 37?C. 5. After the medium reaches 37?C, add carbenicillin to 50 "g/ml (preferred antibiotic) or add ampicillin to 200 "g/ml. Mix on stir plate. 6. To each bottle add 4 %&105 to 6 %&105 primary cDNA transformants (colonies from original library), and mix thoroughly on a stir plate for 2 min. Tighten caps. 7. Place bottles in an ice-water bath (0?C) such that the water in the bath is at the same level as the medium in the bottle. Incubate for 1 h in the ice bath. 8. Gently remove bottles from the ice bath and wipe the water off the outside of the bottles. Loosen bottle caps. Place these bottles in a gravity flow incubator set at 30?C. 9. Incubate these bottles for 40-45 h without disturbance. Place a centrifuge rotor at room temperature the morning of harvest. 10. Pour contents of bottles into centrifuge bottles and centrifuge at 10,400 * g for 20 min at room temperature 11. Pour off the supernatant. 12. Resuspend the cells in a total of 100 ml 2X LB Glycerol (12.5%). Remove two 100-"l aliquots for plating, further analysis, and colony estimate. Cells can be filtered through sterile cheesecloth to remove agarose clumps if present. 13. Subdivide the cells into ~10-ml aliquots and store at -70?C. It is also useful to make a number of 1-ml and 100-"l aliquots. 14. Frozen cells can be used to prepare DNA for GENETRAPPER(&experiments, for plating to screen, or can be further amplified in liquid at 30?C to obtain DNA. Use 2.5 %&109 cells/100 ml growth medium for further expansion of the library. 32 Media 2X LB Component Amount/liter 5 Bacto-Tryptone................................................................. 20 g Bacto-Yeast Extract.......................................................... 10 g NaCl ................................................................................. 10 g 2X LB Glycerol (12.5%) Component Amount/200 ml 2X LB............................................................................ 175 ml Glycerol (100%)...............................................................25 ml Filter-sterilize and store for up to 2 months at room temperature. Amplifying the pCMV-Script? cDNA Library The primary library may now be plated and screened; however, amplification of the library is desirable to produce a large and stable quantity of the library. Do not perform more than one round of amplification, as slower-growing clones may be significantly underrepresented. Stratagene recommends amplifying plasmid libraries in 500-ml bottles of 2* LB agarose? using the semi-solid amplification method.4, 5 The libraries are amplified in suspension which allows for three-dimensional, uniform colony growth. This reduces the potential for under-representation of particular clones due to the overgrowth of some colonies during the expansion process that accompanies direct plating methods. Note Each 500-ml bottle of 2* LB agarose can accommodate ~5 * 105 primary cfu. To amplify a library of 1 * 106 primary cfu, two bottles are necessary. Note Stratagene has determined that the use of SeaPrep? agarose is critical to ensure performance (See Additional Materials Required). 1. On a heated stir plate, using a large stirbar, combine 1.35g of SeaPrep agarose with 450 ml of 2* LB? in each 500-ml autoclavable bottle. Heat and stir until the agarose is in solution. 2. Autoclave the bottles and stir bars for 30 minutes. 3. Allow the bottles to cool to 37?C in a water bath (~1 hour). 4. Transfer the bottles to a stir plate and add 50 *g/ml of kanamycin. 5. Add up to 5 * 105 cfu/bottle of primary library and stir for several minutes. Note The bottles must be handled very carefully at this stage. Avoid swirling or bumping the bottles as this may cause the microcolonies to fall out of suspension. The bottles must be incubated without disturbance or representation of the amplified library may be compromised. 6. Tighten the bottle caps and incubate the bottles for 1 hour in an ice water bath (0?C). The water level in the ice bath should be even with the media level in the bottle. 7. Carefully remove the bottles from the ice bath and gently dry the bottles. Loosen the bottle caps and incubate for 40-45 hours at 30?C. (Incubation at 30?C reduces under-representation of slower growing clones.) ? See Preparation of Media and Reagents. pCMV-Script? XR cDNA Library Construction Kit 8. Pour the contents of the bottles into sterile 250-ml centrifuge bottles and spin at 10,000 * g for 20 minutes at room temperature. (Equilibrate the rotor to room temperature several hours prior to centrifugation. Using rotors stored at 4?C will cause the agar to solidify.) 9. Remove the semi-solid agarose supernatant and resuspend the pellets in 25 ml of 2* LB-glycerol (12.5%) (see Preparation of Media and Reagents) per 250-ml centrifuge bottle. Remove 100 *l for estimation of library titer and further characterization. Pipet the remainder into 1-ml aliquots. Store at -80?C. 10. Perform 6 serial dilutions with 100 *l of the amplified library diluted into 900 *l of LB medium. Plate 10 *l of the 10-5 and 10-6 dilutions onto selection plates. Amplification of a primary library containing 1 * 106 total transformants should result in a stable library of at least 1 * 109 total transformants. pCMV-Script? XR cDNA Library Construction Kit -- Ben Holt Assistant Professor University of Oklahoma Department of Botany and Microbiology GLCH Rm 219 770 Van Vleet Oval Norman, OK 73019 Phone (405)325-9018 FAX (405)325-7619 http://www.ou.edu/cas/botany-micro/faculty/holt.html From ericl89 from hotmail.com Sat Apr 4 11:09:44 2009 From: ericl89 from hotmail.com (Eric Lam) Date: Mon Apr 6 21:16:33 2009 Subject: [Arabidopsis] Postdoc researcher position available immediately Message-ID: A POSTDOCTORAL POSITION is available at the Biotech Center=2C Rutgers Unive= rsity=2C in the laboratory of Prof. Eric Lam (http://aesop.rutgers.edu/~lam= lab) for a highly motivated scientist interested in studying chromatin orga= nization and stress responses in higher plants. We have established a set = of 277 mapped lines of novel transposants in Arabidopsis with single Ds ins= ertions that allows functional and structural characterization of global ep= igenetic control in living plants. Together with the recently established = SOLiD NextGen sequencing capability in Rutgers for multiplexed ChIP-seq and= small RNA analyses=2C our unique resource promise to reveal interesting ne= w insights to the complex network of the epigenome and stress responses usi= ng Arabidopsis and grasses as paradigms. The successful applicant will app= ly these new tools to examine global epigenetic control as a function of de= velopment and in response to stresses.=20 The ideal applicant should have prior experience with molecular (ChIP assay= =2C PCR=2C cloning=2C Northern blotting=2C etc.) and biochemical (Western b= lotting=2C enzyme assay=2C etc) techniques=2C advanced microscopy=2C and/or= chemical biology approaches. Knowledge of epigenetic mechanisms and some b= ioformatics skills are desirable but not required. If you are interested i= n this position=2C please email a cover-letter stating your research intere= sts=2C a curriculum vitae=2C and contact information for three references t= o: Prof. Eric Lam=2C ericL89@hotmail.com The Biotech Center at Rutgers University is located in New Brunswick=2C= New Jersey=2C and enjoys a convenient location close to the famous Jersey = Shore and Pocono Mountains while also easily accessible (within 1 hour by c= ar or train) to metropolitan areas such as New York City and Philadelphia. = The Biotech Center at the School of Environmental and Biological Sciences = in the Cook Campus is well-equipped with advanced instrumentations for mole= cular biology=2C biochemistry and microscopy. Rutgers University is an equ= al opportunity employer that strongly encourages underrepresented groups to= apply for open positions. =20 Related Publications: "Defining the functional network of epigenetic regulators in Arabidopsis th= aliana" Molecular Plant=2C EPub online: Apr. 2009=3B DOI: 10.1093/mp/ssp017 "Genome-wide transposon tagging reveals location-dependent effects on trans= cription and chromatin organization in Arabidopsis"=20 Plant J.=2C Published Online: Apr 12 2008=3B DOI: 10.1111/j.1365-313X.2008= .03517.x "Bax Inhibitor-1 modulates endoplasmic reticulum stress-mediated programmed= cell death in Arabidopsis"=20 J. Biol. Chem.=2C 2008=3B 283: 3200-3210. =93DNA hypomethylation reduces homologous pairing of inserted tandem repeat= arrays in somatic nuclei of Arabidopsis thaliana=94 Plant J.=2C 2005 Nov=3B44(4): 531-40. "Visualizing chromosome structure/organization" Annual Rev. of Plant Biology=2C 2004=3B 55: 537-554. Prof. Eric Lam=20 Director=2C Biotechnology Center for Agriculture=20 and the Environment Professor of Plant Biology and Pathology=20 Rutgers=2C The State University of New Jersey=2C U.S.A. Email: ericL89@hotmail.com=3B Lam@aesop.rutgers.edu=20 From hideoma from ibrc.or.jp Mon Apr 6 05:32:27 2009 From: hideoma from ibrc.or.jp (Hideo Matsumura) Date: Mon Apr 6 21:17:42 2009 Subject: [Arabidopsis] PosDoc position Message-ID: Positions opens for a post-doctoral fellow Subject: rice genetics/genomics Term: One year starting from July, 2009; subject to extension up to a total of three years Deadline of application: 11 May, 2009 Contact person for further information: Dr. Hideo Matsumura (hideoma@ibrc.or.jp ) Applicants should send 1. Curriculum vitae 2. A list of publications (with reprints of recent publications) 3. A letter of self introduction to Dr. Hideo Matsumura Iwate Biotechnology Research Center 22-174-4, Narita, Kitakami, 024-0003 Iwate, Japan e-mail for application: hideoma@ibrc.or.jp We are seeking a competent post-doctoral scientist who will be in charge of rice genetic studies at Iwate Biotechnology Research Center (IBRC). He/she is expected to carry out isolation and characterization of rice genes controlling important agronomic traits (cold tolerance, blast disease resistance), making full use of the rice genomic information. Please see our Home Page (http://www.ibrc.or.jp/English/rice/main.html). ------------------------------------ Hideo Matsumura Iwate Biotechnology Research Center Narita22-174-4 Kitakami Iwate 024-0003 Japan Tel +81-197-68-2911 Fax +81-197-68-3881 ------------------------------------ From sanjaysingh765 from gmail.com Sun Apr 5 10:20:39 2009 From: sanjaysingh765 from gmail.com (chunnu) Date: Mon Apr 6 21:18:43 2009 Subject: [Arabidopsis] eoxn/intron boundry prediction Message-ID: <179e037d-f9d6-4b5d-9cb1-18982a48b217@b7g2000pre.googlegroups.com> hi there, i m looking for a eoxn/intron boundry prediction tool especially for arabidopsis and oryza.although there are nuber of tools available for this but they didn't produce the grphical outputs means they didn't show the region of exon/introns on input. can anyone help me regardng this. regards sanjay From vnunes from igc.gulbenkian.pt Mon Apr 6 05:06:37 2009 From: vnunes from igc.gulbenkian.pt (Vera Nunes) Date: Mon Apr 6 21:25:49 2009 Subject: [Arabidopsis] Soil sterelization Message-ID: <1239012397.49d9d42d1e3be@webmail.igc.gulbenkian.pt> Hello, First of all thanks a lot for your answers to my last question. Since autoclaving the soil it isn't feasible in our lab, does anyone knows another method that we could use to sterilize it? Or at least minimize the chances of fungal growth? Thanks! Vera Nunes From jdfriesner from ucdavis.edu Tue Apr 7 18:04:33 2009 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Tue Apr 7 18:48:47 2009 Subject: [Arabidopsis] Arabidopsis in Scotland Message-ID: <200904072304.n37N4X3P010499@cucujus.ucdavis.edu> Conference update: More than 650 of your colleagues and friends signed up for the Arabidopsis Conference in Edinburgh (June 30-July 4, 2009) by the March 27th early deadline! This is sure to be a well-attended and memorable event. Don't miss the May 1 final deadline to submit an abstract for poster presentations, or the June 15 final registration deadline. Conference website: http://www.arabidopsis2009.com/ From cxde from iastate.edu Wed Apr 8 09:28:59 2009 From: cxde from iastate.edu (Xi Chen) Date: Wed Apr 8 11:54:27 2009 Subject: [Arabidopsis] A question about how to grow big Arabidopsis plants in MS medium? Message-ID: <8af797d40904080728q41719f30hc8f3b2b948832438@mail.gmail.com> Hello researchers, Sorry to bother you. My Arabidopsis have to grow on the MS medium continuously. Now what I do is that after grown in the petri dish for about one week, I transferred the seedlings into magenta box (sigma, V8505, W ? L ? H 77 mm ? 77 mm ? 97 mm) until to the end. But the problem is that the space and nutrition of magenta box is limited, the plants grown there can't give me the healthy siliques. If I transferred one more time into another magenta box, the nutrition maybe enough, but I would damage the roots of plants easily. Thus, do you guys have any experience to solve this problem? Thanks! Best, Xi From criptoendolito from gmail.com Wed Apr 8 12:16:28 2009 From: criptoendolito from gmail.com (Felipe Aceituno) Date: Wed Apr 8 14:59:31 2009 Subject: [Arabidopsis] soil sterilization Message-ID: <7c82292e0904081016n20bd486al53e0624af0af6f68@mail.gmail.com> Hi Vera and everyone I used to work in a soil microbiology lab. There, they sterilize the soil by gamma radiation, and it worked pretty well Hope its an useful info Felipe Flores Aceituno, MSc Laboratorio de Biotecnologia Departamento de Ingeneria Química y Bioprocesos Facultad de Ingeniería Pontificia Universidad Catolica de Chile 2009/4/7 : > Send Arab-gen mailing list submissions to >        arab-gen@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit >        http://www.bio.net/biomail/listinfo/arab-gen > or, via email, send a message with subject or body 'help' to >        arab-gen-request@net.bio.net > > You can reach the person managing the list at >        arab-gen-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Arab-gen digest..." > > > Today's Topics: > >   1. Semi Solid Attempt 2 (Holt, Ben F. III) >   2. Postdoc researcher position available immediately (Eric Lam) >   3. PosDoc position (Hideo Matsumura) >   4. eoxn/intron boundry prediction (chunnu) >   5. Soil sterelization  (Vera Nunes) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 4 Apr 2009 18:00:48 -0500 > From: "Holt, Ben F. III" > Subject: [Arabidopsis] Semi Solid Attempt 2 > To: Arabidopsis Listserv > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi everyone, > > Apparently there were problems with the last attachment - I went back and checked the message I sent and the attachment was there, so I conclude that the listserv is either blocking these in general or just didn't like the one I sent. > > Anyway, I have attached it here again and I have pasted the two protocols below - sorry, but the pasted protocols below are not very pretty. I had to choose between making them look nice or watching the Final Four. > > Go Heels! > > Ben > > > Additional Protocols > > Note: Carbenicillin reduces satellite > formation. > > Caution: Make sure that the rotor was set > at room temperature for at least 2 h before > adding the bottles. Rotors at 4°C will > cause solidification of agar. > > 5.1 Expansion of Plasmid cDNA Libraries > Semi-solid amplification of primary cDNA transformants minimizes representational > biases that can occur during the expansion of plasmid cDNA libraries (25). Life > Technologies has further modified this protocol such that the amplification is done at > 30°C, helping to stabilize unstable clones (26). > > Caution: Bottles of semi-solid agar containing suspended colonies must be handled > gently and incubated without disturbance. Rough handling or bumping of the > incubator will cause micro colonies to fall out of solution. Incubators that have fans > can cause colonies to fall out of solution. > > 1. > Prepare 2.0 L of 2X LB. Remove 175 ml of the 2X LB to make the 2X LB > Glycerol. > 2. > Place a large stir bar and 1.35 g SeaPrep® agarose into each of four 500-ml > autoclavable bottles. Place bottles on stir plates. With the stir plate turned on, > add 450 ml of 2X LB to each bottle (avoid the formation of large clumps of > agarose). > 3. > Autoclave these bottles of 2X LB agarose for 30 min. > 4. > Cool bottles in 37°C water bath for ~2 h until the medium reaches 37°C. > 5. > After the medium reaches 37°C, add carbenicillin to 50 "g/ml (preferred > antibiotic) or add ampicillin to 200 "g/ml. Mix on stir plate. > 6. > To each bottle add 4 %&105 to 6 %&105 primary cDNA transformants (colonies > from original library), and mix thoroughly on a stir plate for 2 min. Tighten caps. > 7. > Place bottles in an ice-water bath (0°C) such that the water in the bath is at the > same level as the medium in the bottle. Incubate for 1 h in the ice bath. > 8. > Gently remove bottles from the ice bath and wipe the water off the outside of > the bottles. Loosen bottle caps. Place these bottles in a gravity flow incubator > set at 30°C. > 9. > Incubate these bottles for 40-45 h without disturbance. Place a centrifuge rotor > at room temperature the morning of harvest. > 10. > Pour contents of bottles into centrifuge bottles and centrifuge at 10,400 * g for > 20 min at room temperature > 11. > Pour off the supernatant. > 12. > Resuspend the cells in a total of 100 ml 2X LB Glycerol (12.5%). Remove two > 100-"l aliquots for plating, further analysis, and colony estimate. Cells can be > filtered through sterile cheesecloth to remove agarose clumps if present. > 13. > Subdivide the cells into ~10-ml aliquots and store at -70°C. It is also useful to > make a number of 1-ml and 100-"l aliquots. > 14. > Frozen cells can be used to prepare DNA for GENETRAPPER(&experiments, for > plating to screen, or can be further amplified in liquid at 30°C to obtain DNA. > Use 2.5 %&109 cells/100 ml growth medium for further expansion of the library. > 32 > > > > > Media > 2X LB > Component Amount/liter > > 5 > > Bacto-Tryptone................................................................. 20 g > > > Bacto-Yeast Extract.......................................................... 10 g > NaCl ................................................................................. 10 g > > > 2X LB Glycerol (12.5%) > Component Amount/200 ml > > > 2X LB............................................................................ 175 ml > Glycerol (100%)...............................................................25 ml > > > Filter-sterilize and store for up to 2 months at room temperature. > > > > > Amplifying the pCMV-Script® cDNA Library > > The primary library may now be plated and screened; however, > amplification of the library is desirable to produce a large and stable > quantity of the library. Do not perform more than one round of > amplification, as slower-growing clones may be significantly > underrepresented. > > Stratagene recommends amplifying plasmid libraries in 500-ml bottles of 2* > LB agarose§ using the semi-solid amplification method.4, 5 The libraries are > amplified in suspension which allows for three-dimensional, uniform colony > growth. This reduces the potential for under-representation of particular > clones due to the overgrowth of some colonies during the expansion process > that accompanies direct plating methods. > > Note > Each 500-ml bottle of 2* LB agarose can accommodate ~5 * 105 > primary cfu. To amplify a library of 1 * 106 primary cfu, two > bottles are necessary. > > Note > Stratagene has determined that the use of SeaPrep® agarose is > critical to ensure performance (See Additional Materials > Required). > > 1. > On a heated stir plate, using a large stirbar, combine 1.35g of SeaPrep > agarose with 450 ml of 2* LB§ in each 500-ml autoclavable bottle. > Heat and stir until the agarose is in solution. > 2. > Autoclave the bottles and stir bars for 30 minutes. > 3. > Allow the bottles to cool to 37°C in a water bath (~1 hour). > 4. > Transfer the bottles to a stir plate and add 50 *g/ml of kanamycin. > 5. > Add up to 5 * 105 cfu/bottle of primary library and stir for several > minutes. > Note > The bottles must be handled very carefully at this stage. > Avoid swirling or bumping the bottles as this may cause the > microcolonies to fall out of suspension. The bottles must be > incubated without disturbance or representation of the > amplified library may be compromised. > > 6. > Tighten the bottle caps and incubate the bottles for 1 hour in an ice > water bath (0°C). The water level in the ice bath should be even with > the media level in the bottle. > 7. > Carefully remove the bottles from the ice bath and gently dry the > bottles. Loosen the bottle caps and incubate for 40-45 hours at 30°C. > (Incubation at 30°C reduces under-representation of slower growing > clones.) > § See Preparation of Media and Reagents. > > pCMV-Script® XR cDNA Library Construction Kit > > > 8. > Pour the contents of the bottles into sterile 250-ml centrifuge bottles > and spin at 10,000 * g for 20 minutes at room temperature. > (Equilibrate the rotor to room temperature several hours prior to > centrifugation. Using rotors stored at 4°C will cause the agar to > solidify.) > 9. > Remove the semi-solid agarose supernatant and resuspend the pellets in > 25 ml of 2* LB-glycerol (12.5%) (see Preparation of Media and > Reagents) per 250-ml centrifuge bottle. Remove 100 *l for estimation > of library titer and further characterization. Pipet the remainder into > 1-ml aliquots. Store at -80°C. > 10. > Perform 6 serial dilutions with 100 *l of the amplified library diluted > into 900 *l of LB medium. Plate 10 *l of the 10-5 and 10-6 dilutions > onto selection plates. Amplification of a primary library containing > 1 * 106 total transformants should result in a stable library of at least > 1 * 109 total transformants. > pCMV-Script® XR cDNA Library Construction Kit > > > > > > > -- > Ben Holt > Assistant Professor > University of Oklahoma > Department of Botany and Microbiology > GLCH Rm 219 > 770 Van Vleet Oval > Norman, OK  73019 > Phone (405)325-9018 > FAX   (405)325-7619 > http://www.ou.edu/cas/botany-micro/faculty/holt.html > > > ------------------------------ > > Message: 2 > Date: Sat, 4 Apr 2009 12:09:44 -0400 > From: Eric Lam > Subject: [Arabidopsis] Postdoc researcher position available >        immediately > To: Arabidopsis Network > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > > A POSTDOCTORAL POSITION is available at the Biotech Center, Rutgers University, in the laboratory of Prof. Eric Lam (http://aesop.rutgers.edu/~lamlab) for a highly motivated scientist interested in studying chromatin organization and stress responses in higher plants.  We have established a set of 277 mapped lines of novel transposants in Arabidopsis with single Ds insertions that allows functional and structural characterization of global epigenetic control in living plants.  Together with the recently established SOLiD NextGen sequencing capability in Rutgers for multiplexed ChIP-seq and small RNA analyses, our unique resource promise to reveal interesting new insights to the complex network of the epigenome and stress responses using Arabidopsis and grasses as paradigms.  The successful applicant will apply these new tools to examine global epigenetic control as a function of development and in response to stresses. > The ideal applicant should have prior experience with molecular (ChIP assay, PCR, cloning, Northern blotting, etc.) and biochemical (Western blotting, enzyme assay, etc) techniques, advanced microscopy, and/or chemical biology approaches. Knowledge of epigenetic mechanisms and some bioformatics skills are desirable but not required.  If you are interested in this position, please email a cover-letter stating your research interests, a curriculum vitae, and contact information for three references to: Prof. Eric Lam, ericL89@hotmail.com >    The Biotech Center at Rutgers University is located in New Brunswick, New Jersey, and enjoys a convenient location close to the famous Jersey Shore and Pocono Mountains while also easily accessible (within 1 hour by car or train) to metropolitan areas such as New York City and Philadelphia.  The Biotech Center at the School of Environmental and Biological Sciences in the Cook Campus is well-equipped with advanced instrumentations for molecular biology, biochemistry and microscopy.  Rutgers University is an equal opportunity employer that strongly encourages underrepresented groups to apply for open positions. > > Related Publications: > > "Defining the functional network of epigenetic regulators in Arabidopsis thaliana" > Molecular Plant, EPub online: Apr. 2009; DOI: 10.1093/mp/ssp017 > > "Genome-wide transposon tagging reveals location-dependent effects on transcription and chromatin organization in Arabidopsis" > Plant J.,  Published Online: Apr 12 2008; DOI: 10.1111/j.1365-313X.2008.03517.x > > "Bax Inhibitor-1 modulates endoplasmic reticulum stress-mediated programmed cell death in Arabidopsis" > J. Biol. Chem., 2008; 283: 3200-3210. > > “DNA hypomethylation reduces homologous pairing of inserted tandem repeat arrays in somatic nuclei of Arabidopsis thaliana” > Plant J., 2005 Nov;44(4): 531-40. > > "Visualizing chromosome structure/organization" > Annual Rev. of Plant Biology, 2004; 55: 537-554. > > > Prof. Eric Lam > > Director, Biotechnology Center for Agriculture >      and the Environment > Professor of Plant Biology and Pathology > > Rutgers, The State University of New Jersey, U.S.A. > > > > Email: ericL89@hotmail.com; Lam@aesop.rutgers.edu > > > > ------------------------------ > > Message: 3 > Date: Mon, 06 Apr 2009 19:32:27 +0900 > From: Hideo Matsumura > Subject: [Arabidopsis] PosDoc position > To: > Message-ID: > Content-Type: text/plain;       charset="ISO-2022-JP" > > Positions opens for a post-doctoral fellow > > Subject:  rice genetics/genomics > Term: One year starting from July, 2009; subject to extension up to a total > of three years > > Deadline of application: 11 May, 2009 > > Contact person for further information: > Dr. Hideo Matsumura  (hideoma@ibrc.or.jp ) > > Applicants should send > > 1. Curriculum vitae > 2. A list of publications (with reprints of recent publications) > 3. A letter of self introduction > > to > > Dr. Hideo Matsumura > Iwate Biotechnology Research Center > 22-174-4, Narita, Kitakami, 024-0003 > Iwate, Japan > e-mail for application: hideoma@ibrc.or.jp > >  We are seeking a competent post-doctoral scientist who will be in charge of > rice genetic studies at Iwate Biotechnology Research Center (IBRC).  He/she > is expected to carry out isolation and characterization of rice genes > controlling important agronomic traits (cold tolerance, blast disease > resistance), making full use of the rice genomic information.  Please see > our Home Page (http://www.ibrc.or.jp/English/rice/main.html). > > > > ------------------------------------ > Hideo Matsumura > > Iwate Biotechnology Research Center > > Narita22-174-4 > Kitakami > Iwate 024-0003 > Japan > > Tel +81-197-68-2911 > Fax +81-197-68-3881 > ------------------------------------ > > > > > > ------------------------------ > > Message: 4 > Date: Sun, 5 Apr 2009 08:20:39 -0700 (PDT) > From: chunnu > Subject: [Arabidopsis] eoxn/intron boundry prediction > To: bionet-genome-arabidopsis@moderators.isc.org > Message-ID: >        <179e037d-f9d6-4b5d-9cb1-18982a48b217@b7g2000pre.googlegroups.com> > Content-Type: text/plain; charset=ISO-8859-1 > > hi there, > i m looking for a eoxn/intron boundry prediction tool especially for > arabidopsis and oryza.although there are nuber of tools available for > this but they didn't produce the grphical outputs means they didn't > show the region of exon/introns on input. can anyone help me regardng > this. > regards > sanjay > > > > ------------------------------ > > Message: 5 > Date: Mon, 06 Apr 2009 11:06:37 +0100 > From: Vera Nunes > Subject: [Arabidopsis] Soil sterelization > To: arab-gen@magpie.bio.indiana.edu > Message-ID: <1239012397.49d9d42d1e3be@webmail.igc.gulbenkian.pt> > Content-Type: text/plain; charset=UTF-8 > > > > Hello, > > First of all thanks a lot for your answers to my last question. Since > autoclaving the soil it isn't feasible in our lab, does anyone knows another > method that we could use to sterilize it? Or at least minimize the chances of > fungal growth? > > Thanks! > > Vera Nunes > > > > ------------------------------ > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > > End of Arab-gen Digest, Vol 48, Issue 4 > *************************************** > -- From courses from embl.de Thu Apr 9 06:41:57 2009 From: courses from embl.de (EMBL Courses & Conferences) Date: Thu Apr 9 18:28:21 2009 Subject: [Arabidopsis] The joint EMBL/Affymetrix workshop on whole transcript microarray data analysis Message-ID: <200904091143.n39Bh8h14947@net.bio.net> EMBL Course Announcement Dear Colleagues, We are writing to announce the The joint EMBL/Affymetrix workshop on whole transcript microarray data analysis at the European Molecular Biology Laboratory (Heidelberg, Germany) from 4 - 7 May 2009. Please visit the web site for full conference details: www.embl.de/courses/affymetrix/2009 Registration deadline: 19 April 2009 Please circulate this announcement to interested members and groups within your institute. We are looking forward to welcoming you at EMBL Heidelberg. Best wishes, Bettina Sch?fer Conference Officer Course and Conference Office European Molecular Biology Laboratory Meyerhofstr. 1 D-69117 Heidelberg Tel: +49-6221-387 8397 Fax: +49-6221-387 8836 Email: schaefer@embl.de For full event listings please visit our website: www.embl.org/events If you do not wish to receive any further event announcements, please reply with "unsubscribe". From kbrowning from mail.utexas.edu Fri Apr 10 10:30:26 2009 From: kbrowning from mail.utexas.edu (Karen Browning) Date: Fri Apr 10 12:33:53 2009 Subject: [Arabidopsis] Gateway compatible binary vector? Message-ID: <49DF6612.2020302@mail.utexas.edu> Does anyone have a Gateway LR compatible binary destination vector with herbicide resistance (bar)? Thank you. -- *********************************************************** Dr. Karen S. Browning Associate Professor Department of Chemistry and Biochemistry The University of Texas at Austin Welch Hall 5.248, 24th and Speedway (Overnight Deliveries) 1 University Station A5300 (Regular Mail) Austin TX 78712-0165 kbrowning@mail.utexas.edu http://research.cm.utexas.edu/kbrowning/ 512-471-4562 512-471-8696 FAX From oscadd from gmail.com Fri Apr 10 11:22:24 2009 From: oscadd from gmail.com (Raghava) Date: Sat Apr 11 22:31:02 2009 Subject: [Arabidopsis] Prediction of guide strand of microRNAs Message-ID: Dear Colleagues We have developed a method for predicting "guide strand of microRNAs from its sequence and secondary structure" it is published in BMC Bioinformatics see http://www.biomedcentral.com/1471-2105/10/105 . Though it is developed to discriminate for guide and passenger strand of miRNA but it can be used to design siRNA. I hope this information will be useful for bioinformatics community. With Regards Raghava #====================================================== # Dr G P S Raghava FASc, FNASc, Scientist and Head Bioinformatics Centre # # Institute of Microbial Technology, Sector-39A, Chandigarh, India # # Phone: +91-172-2690557, Fax: +91-172-2690632 # # Eadd: http://www.imtech.res.in/raghava/ raghava@imtech.res.in # #=====================================================- From mchatterjee from benchbio.com Sun Apr 12 09:09:12 2009 From: mchatterjee from benchbio.com (Manash Chatterjee) Date: Sun Apr 12 22:40:03 2009 Subject: [Arabidopsis] Job posting-Crop Biotechnologist, India Message-ID: <7EED0F117426495CBBD8BA41B8E9988B@MANASH> Dear Sir/Madam, Could you please post this advert under Arabidopsis newsgroup. Thanks POSITION: Senior Scientist, Crop Biotechnology, India Bench Bio Ltd. is a functional genomics start-up company established in 2007 that aims to create and discover novel alleles of genes of agricultural and consumer value using TILLING and eco-TILLING method. It is focused on exploiting the genome sequencing projects to understand the fundamental processes of plant growth and development and to discover genes of value offering real added value potential. Bench Bio is interested in individuals with exceptional creativity, motivation and scientific abilities to join this challenging and exciting opportunity. We offer a competitive salary and benefit package. All application treated confidentially. Positions will remain open until suitable candidates are found. Job Description 1) Provides management, scientific leadership and creativity when initiating, designing, and executing research projects. 2) Works in conjunction with other scientists to establish and execute project goals 3) Delegating authority to, supervising and setting goals for scientists 4) Development of plans and programs to improve operations to ensure maximum output. 5) Good communication skills and liason with external partners in India and abroad. 6) Excellent People and organizational skills 7) Ability to manage Research Staff 8) Strong knowledge and experience in molecular biology, genetics, plant breeding and bioinformatics tools. 9) Remains current on new technological developments 10) May contribute to scientific publications, conferences and patent applications. 11) Provides orientation and training, supervises, and evaluates employee performance. 12) Monitors research progress and meets with participating researchers to identify products and opportunities impacting the business unit and provides solutions to problems. 13) Is effective at communication, partnership, and knowledge transfer. Job Requirements 1) PhD in Plant Biology/Breeding/Molecular Biology/Genetics/Biotechnology or related field with two years or more of R&D experience in an industrial setting or academic lab. Information and Application Please submit your resume with cover letter to BenchBio Private Limited Attn. HR/GL01 e-mail: info@benchbio.com Email submission is encouraged Or addressed to BenchBio Private Limited C/O Jai Research Foundation (JRF) Near Daman Ganga Bridge, N.H.No. 8, Valvada, District Valsad Gujarat India- 396 108 Tel: +91-260 6544244 Fax: +91 22- 6646 6908 Please see more information about the company at www.benchbio.com Best regards Dr Manash Chatterjee Founder & Director BenchBio Private Limited C/O Jai Research Foundation (JRF) Near Daman Ganga Bridge, N.H.No. 8, Valvada, District Valsad Gujarat India- 396 108 Tel: +91-260 6544244 Mobile : +91 9909945811 Fax: +91 22- 6646 6908 E-mail: mchatterjee@benchbio.com www.benchbio.com From dfernand from wisc.edu Mon Apr 13 15:36:45 2009 From: dfernand from wisc.edu (Donna Fernandez) Date: Mon Apr 13 18:04:17 2009 Subject: [Arabidopsis] Postdoctoral Position- Madison, WI Message-ID: Postdoctoral Research Position University of Wisconsin-Madison A full time Research Associate position is available immediately in the laboratory of Donna Fernandez, Department of Botany, to investigate genetic and physical interactions between MADS-domain transcription factors involved in the control of flowering time. We are using genetic and molecular approaches to define the regulatory roles of AGL15 and AGL18 (Plant J. 50: 1007-1019, 2007) in relation to the roles of other MADS-domain floral repressors. The successful candidate will become a member of the vibrant plant research community in Madison. The initial appointment will be for one year, with a possibility of extension by one or more years. A Ph.D. degree in molecular biology, genetics, or a related field is required. Previous experience with plants is desirable. Candidates must have excellent communication skills. Interested applicants should send a CV, list of publications, brief summary of previous research experience, and contact information for three references by e-mail to dfernand@wisc.edu. Donna Fernandez Professor Dept. of Botany 430 Lincoln Drive University of Wisconsin-Madison Madison, WI 53706-1381 USA Phone: (608) 262-9033 FAX: (608) 262-7509 e-mail: dfernand@wisc.edu From eshpak from utk.edu Mon Apr 13 15:07:08 2009 From: eshpak from utk.edu (Shpak, Elena D) Date: Mon Apr 13 18:04:58 2009 Subject: [Arabidopsis] Post-doctoral position - plant development Message-ID: Post-doctoral position in Plant development A postdoctoral position in Plant development/Genetics/Molecular biology is available at University of Tennessee, Knoxville. The NSF funded project will focus on ERECTA receptor-like kinase signaling in Arabidopsis. (Shpak et al., 2004 Development 131, p1491-1501 and Shpak et al., 2005 Science 309, p290-293). Applicants with strong experience in molecular biology, biochemistry and cell biology are encouraged to apply. Experience with Arabidopsis is helpful, but not required. This position is available for 3 years. To apply, send a cover letter describing your interests, CV, as well as names and contact information of three references to eshpak@utk.edu. Elena D Shpak Assistant professor Biochemistry, Cellular & Molecular Biology department University Of Tennessee, Knoxville (http://web.bio.utk.edu/bcmb/faculty/Individual_Faculty_Pages/Shpak_Elen a/Shpak_Elena.html) From gleon from unab.cl Mon Apr 13 16:39:10 2009 From: gleon from unab.cl (=?iso-8859-1?Q?Gabriel_Le=F3n?=) Date: Mon Apr 13 18:05:40 2009 Subject: [Arabidopsis] BASTA resistance gene silencing Message-ID: Dear Colleagues, Does anybody experienced BASTA resistance gene silencing? We are screening a large collection of T-DNA knockouts (mainly SAIL and SM lines), and for some lines no resistant seedlings were rescued, even after sowing 70 seeds (all germinated, the phenotype is compatible with BASTA sensitive seedlings). This could be produced by a lethal mutation that segregates in a distorted way, but we are wondering if anybody has experienced BASTA gene silencing, as described for kanamycing in some Salk lines. Thank you, Gabriel __ Dr. Gabriel Le?n Profesor Asistente Centro de Biotecnolog?a Vegetal Universidad Andr?s Bello Rep?blica 217, 1 piso Santiago - CHILE Tel: +56-2-7703035 Fax: +56-2-6615832 www.unab.cl From abidur from iwate-u.ac.jp Tue Apr 14 00:01:41 2009 From: abidur from iwate-u.ac.jp (Abidur Rahman) Date: Tue Apr 14 11:17:40 2009 Subject: [Arabidopsis] job posting- Faculty position at Iwate University , Japan Message-ID: <6C38ECDF990E4BE481C4A2CFCFB7EABF@Abidurnotebook> Dear Moderator, I would appreciate if you please post the following job advertisement. Best regards, Abidur ------------------------------------------------------------------------------------------------------------------------------------------------------------- Faculty Position in Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Japan The Cryobiofrontier Research Center (CRC) at Iwate University, Morioka, Japan invites applications for Professor Position in the field of cold adaptation biology. CRC is an affiliated research center of Faculty of Agriculture, Iwate University and founded on 2008. The center is currently hosting seven faculty members, whose research are mainly focused to explore "various unique biological phenomena occurring under cold environment." Title of position: Professor Starting date: October 1, 2009 (negotiable). Requirements: The candidate should have a Ph. D. and postdoctoral experience in biological science with a proven track record in research, teaching (bachelor, master, and doctoral courses) and management. The position also requires English fluency. The successful applicant is expected to 1) develop an extramurally funded, internationally recognized research program on molecular/physiological/biochemical /cellular or eco-physiological mechanisms of adaptation and responses to cold environment. Possible research areas include, but are not limited to, temperature sensing, signal transduction, regulation mechanism of gene expression, genetics of cold response and application in breeding. Other original themes and approaches, which may enhance the CRC's research strength and target, would also be welcomed. 2) The appointee is expected to teach a biology-related laboratory course and/or a biology-related lecture class at both undergraduate and graduate levels and 3) mentor both undergraduate and graduate students. Application instructions: The closing date for application is by May 31, 2009. The application must include: 1. Curriculum vitae (including applicant's e-mail address). 2. List of publications, research grants awarded and patents obtained by the applicant. 3. Reprints (or photocopies) of five key papers if available. 4. Names and contact information including e-mail addresses of three references. 5. Statement for previous research accomplishment and future research (approximately 1,500 words each). 6. A brief and concise teaching statement. 7. A cover letter explaining applicant's competence for this position. Salary: Annual salary will be commensurate with individual's experience and in accordance with the provisions of Iwate University. Compensation package (health/dental insurances and others) based on the university regulations will be available. Additional information: 1. To be reviewed for this position, the applicant must have at least 20 refereed publications (original papers, reviews, and books). 2. Detail information on research field/interest of each faculty member of CRC as well as additional information on CRC and/or Iwate University is available upon request. Any queries should be directed to the search committee chair, Dr. Kikukatsu Ito. 3. Decision of the Selection Committee will be notified to all applicants immediately after the first screening. Application documents are non-returnable. 4. Only the short listed candidates will be invited for interview. Please send application or inquiry to: Kikukatsu Ito, Ph.D. Chair, Selection Committee Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University Morioka, Iwate 020-8550, Japan Phone and Fax, +81-19-621-6143; E-mail, kikuito@iwate-u.ac.jp ----------------------------------- Abidur Rahman, Ph.D Assistant Professor Cryobiofrontier Research Center Faculty of Agriculture Iwate University Ueda 3-18-8, Morioka 020-8550 Japan Tel: +81-(0)19-621-6144 Fax:+81-(0)19-621-6243 email: abidur@iwate-u.ac.jp url:http://news7a1.atm.iwate-u.ac.jp/~abidur/ From wd205 from cam.ac.uk Tue Apr 14 05:39:44 2009 From: wd205 from cam.ac.uk (Walter Dewitte) Date: Tue Apr 14 11:18:14 2009 Subject: [Arabidopsis] Fwd: PhD Studentship 2 References: <18E3A370-10A3-4290-AF82-4FD2F236500D@cam.ac.uk> Message-ID: <8052299C-E2E6-4C65-B38E-D8AC9786ED8E@cam.ac.uk> Please use this as the previous message had a spelling mistake. Thanks, Walter Begin forwarded message: > From: Walter Dewitte > Date: 14 April 2009 10:17:49 BST > To: arab-gen@net.bio.net > Subject: PhD Studentship > > PhD Studentship, Prof. Jim Murray, Cardiff University > 4 year PhD Studentship funded by Bayer CropScience, available to all > UK and EU residents > Manipulation of seed development using cell cycle genes in > Arabidopsis and Brassica > > A prestigious PhD studentship is available working with Prof. Jim > Murray and Dr Walter Dewitte, recently moved to new state-of-the art > facilities in the School of Biosciences, Cardiff University, where > the lab is currently recruiting. The project is fully funded by > Bayer CropScience (http://www.bayercropscience.com/) for 4 years > from October 2009 or earlier, with an annual enhanced stipend of > ?15790 (rising to ?17450 by Year 4). The studentship and stipend is > open to all UK and EU nationals with full funding of both fees and > maintenance stipend. The project builds on existing collaborations > between Bayer CropScience and the Murray lab, and aims to use cell > cycle genes to control seed size and development in Arabidopsis and > Brassica. The project will be based in Cardiff but also involve time > in Gent, Belgium working with Bayer CropScience. > For more information on the Murray lab, see http://www.murraylab.org.uk > and on the exciting capital city of Wales and beautiful landscapes > see http://www.VisitCardiff.com/About-Cardiff.html and http://www.visitsouthwales.com/ > . A equivalent to a upper second class or Masters degree in Plant > Biology, Biochemistry/ Molecular Biology or related subjects is > required, and previous practical experience in plant biology would > be highly desirable. To apply, please send both a hardcopy and email > of a full CV, a description of past lab experience and projects > undertaken and full contacts (including emails) of two referees to > Prof Jim Murray. Closing date: May 17th 2009, but early application > is encouraged. > murrayja1@cardiff.ac.uk > Cardiff School of Biosciences > Museum Avenue > Cardiff CF10 3AX > Wales, UK > Tel: +44 (0)2920 876676 > Dr. Walter Dewitte > > Cardiff School of Biosciences > Biomedical Sciences Building > Museum Avenue > Cardiff CF10 3AX > Wales, UK > > email: DewitteW@cardiff.ac.uk > Dr. Walter Dewitte Cardiff School of Biosciences Biomedical Sciences Building Museum Avenue Cardiff CF10 3AX Wales, UK email: DewitteW@cardiff.ac.uk From kbrowning from mail.utexas.edu Wed Apr 15 17:36:41 2009 From: kbrowning from mail.utexas.edu (Karen Browning) Date: Wed Apr 15 18:22:19 2009 Subject: [Arabidopsis] Gateway compatible binary vector--RESPONSES to query Message-ID: <49E66179.1010902@mail.utexas.edu> Thank you for the many replies to my query on Gateway vectors I have pasted them below as they may be useful to others. Best, Karen "You might want to look at the pEarleyGate vectors and see if any of them work for you. You can get them all at ABRC. http://www.biology.wustl.edu/pikaard/pEarleyGate%20plasmid%20vectors%20copy/ Table%20of%20vectors.html" "I have used Gateway destination vector for my experiment published recently. We have a Gateway LR binary destination vector which carries gentamycin resistant gene (under 35S promoter) as a marker instead of Bar gene. If you contact Danny Schnell in UMass, (dschnell@biochem.umass.edu), you can get a plasmid DNA. http://www.ncbi.nlm.nih.gov/pubmed/19168645?dopt=Abstract" " The set of vectors by Curtis and Grossniklaus (Plant Phys 133:462) are Gateway compatible. I have a few, but they had us to sign an agreement not to distribute. If you get them to agree, I can send you some like pMDC123" liq@muohio.edu "Please check the homepage of Karimi et al. at the University Gent in Belgium. You will find it if you gooogle for Gent and Gateway. They have virtually everything one may wish for plant-related GATEWAY vectors," "check this web page http://www.biology.wustl.edu/pikaard/pEarleyGate%20plasmid%20vectors%20copy/Table%20of%20vectors.html these vectors are available at the TAIR" "I have a pAMPAT derivative with GATEWAY cassette and BASTA resitance. If you still need one, please contact me, then could send some plasmid."Anke.Hueser@uni-koeln.de "check this site http://www.psb.ugent.be/gateway/index.php" "you can use one plasmid that had been designed by Richard Curtis http://botserv1.uzh.ch/home/grossnik/curtisvector/index_2.html Although most of them are Hygromycine resistant, one is Basta R (pMDC123). You can order them through ABRC. For full references: Curtis et al, Plant Physiol 133-pp462" "Do you want the single gene gateway vector or multigene gateway vector with herbicide gene. both are available for you from plant systems biology. its free but they will ask for the shiping charges etc. if you wont the mutigene gateway vector we have developed another herbicide gene much effecient than bar.also here you 'll have to search for the which DONOR vectors you are working on and also the compatible binary vector."ramu.tiger123@gmail.com -- *********************************************************** Dr. Karen S. Browning Associate Professor Department of Chemistry and Biochemistry The University of Texas at Austin Welch Hall 5.248, 24th and Speedway (Overnight Deliveries) 1 University Station A5300 (Regular Mail) Austin TX 78712-0165 kbrowning@mail.utexas.edu http://research.cm.utexas.edu/kbrowning/ 512-471-4562 512-471-8696 FAX From Jean-Luc.Gallois from avignon.inra.fr Thu Apr 16 03:07:04 2009 From: Jean-Luc.Gallois from avignon.inra.fr (Gallois) Date: Thu Apr 16 20:56:53 2009 Subject: [Arabidopsis] Non kanamycin-resistant pENTR vector Message-ID: <49E6E728.2020909@avignon.inra.fr> Dear colleagues, as I want to do gateway cloning in a kan-resistant binary vector, I am looking for an entry vector with a non-kanamycin resistance. Ideally, this entry clone would look like a basic pENTR (as pENTR4 sold by invitrogen) with a good MCS between attL1 and attL2 but with a gentamicin resistance gene. Although invitrogen has designed both KanR and GentaR type of plasmids for pDONR (pDONR201 and pDONR207) I could not find a similar set of plasmids for pENTR. If anyone knows about such a plasmid or had designed one and would be likely to share it, I would be really grateful, Regards Jean-Luc Jean-Luc GALLOIS INRA-UR 1052 G?n?tique et Am?lioration des Fruits et L?gumes Dom. St Maurice, BP94 84143 Montfavet cedex, France From meier.56 from osu.edu Wed Apr 15 16:40:00 2009 From: meier.56 from osu.edu (Iris Meier) Date: Thu Apr 16 20:57:31 2009 Subject: [Arabidopsis] Postdoctoral Position available Message-ID: POSTDOCTORAL POSITION An NSF-funded postdoctoral position is available immediately to investigate the function of nuclear pore proteins during the plant cell cycle. We investigate proteins associated with the inner and outer surface of the Arabidopsis nuclear pore that are involved in the Ran cycle, nucleocytoplasmic trafficking, and sumoylation of nuclear proteins. Currently, we are most excited about newly discovered cell-cycle functions of these proteins away from the nuclear pore. For our recent publications see: Xu et al. (2008) Proc Natl Acad Sci U S A. 105(47):18637-42; Zhao et al. (2008) Plant Cell 20, 1639-1651; Xu et al. (2007) Curr Biol. 17: 1157-1163; Xu et al. (2007) Plant Cell 19: 1537-1548; Xu and Meier (2008) Trends in Plant Sci. 13, 1-50; and Brkljacic and Meier (2009) Curr. Opin. Plant Biol 12, 87-95. More recent, unpublished findings and projects will be discussed with interviewing candidates. We are located at The Ohio State University in Columbus, Ohio. For more information on our scientific environment and our research facilities, please see: Department of Plant Cellular and Molecular Biology: http://www.biosci.ohio-state.edu/~plantbio/plantbio.html; Department of Molecular Genetics: http://www.osumolgen.org; and the Plant Molecular Biology and Biotechnology Program: http://pmbb.osu.edu/index.php; Columbus is a safe and green urban center of one million with an international flavor and a pleasant level of sophistication. To apply, please send a CV, a brief statement of research experience and research interests, and the names of three references (including address, phone, and email) to meier.56@osu.edu. Successful postdoctoral candidates will have a Ph.D., solid experience in either Arabidopsis molecular genetics or state-of-the-art cell biology, and a proven record of productivity, as evidenced by publications in internationally recognized journals. -- ______________________________ Dr. Iris Meier Professor Plant Cellular and Molecular Biology The Ohio State University 520 Aronoff Laboratory 318 W 12th Avenue Columbus, OH 43210 USA +1 614 292 8323 meier.56@osu.edu From sanjaysingh765 from gmail.com Sat Apr 18 06:48:50 2009 From: sanjaysingh765 from gmail.com (chunnu) Date: Sat Apr 18 17:13:06 2009 Subject: [Arabidopsis] KA/Ks alculation Message-ID: HI, I wanna caluculate Ka and ks ratio for a set of arabiodpsis and oryza sequence to chrck the kind of selection pressure.But i am little bit confused about the reference sequence.May I take the respective human sequences.Woult it be wise decision??? Can any one help me in this regards sanjay From shuly from cshl.edu Sun Apr 19 17:08:31 2009 From: shuly from cshl.edu (Shuly Avraham) Date: Mon Apr 20 01:13:39 2009 Subject: [Arabidopsis] Plant Ontology Database #0409 Release Message-ID: <39B39A9A-F5F2-46AE-B76E-2235DE7E40D4@cshl.edu> Plant Ontology Consortium (POC) is excited to announce the release #0409 (April 2009) of the Plant Ontology Database. In this release, we bring you 26625 gene annotations from TAIR, Gramene, SGN and MaizeGDB, 8558 QTL annotations from Gramene, 9832 germplasm associations from SGN, MaizeGDB and NASC. The following number of annotations were added for the first time: 16016 gene annotations from TAIR, 3 gene annotations from Gramene, 2 gene annotations from SGN and 3928 germplasm annotations from MaizeGDB. For genes curated by TAIR and SGN, you may also find links to their Gene Ontology (GO) pages through PO browser. The new ontology files and the database dump are available for download. To submit plant ontology term requests, we encourage researchers to use SourceForge PO tracker. The Plant Ontology Consortium web: http:www.plantontology.org e-mail: po-dev at plantontology.org The project is funded by National Science Foundation, USA, (Grant No. DBI-0703908) From kv35 from cornell.edu Mon Apr 20 08:20:58 2009 From: kv35 from cornell.edu (Klaas van Wijk) Date: Mon Apr 20 11:21:27 2009 Subject: [Arabidopsis] Position in Mass spectrometry (LTQ-Orbitrap) at Cornell (vanWijk lab) Message-ID: <6.2.1.2.2.20090420091308.02e154a0@postoffice6.mail.cornell.edu> Post-doctoral position in Mass spectrometry (LTQ-Orbitrap) Cornell University, Ithaca, NY, USA ? van Wijk lab - [1]http://cbsu.tc.cornell.edu/vanwijk/ Mass spectrometrist. A postdoc position (2-3 years) is available in Mass Spectrometry and Comparative Proteomics. The applicant will operate three mass spectrometers for protein and peptide identification: 1. a MALDI-TOF mass spectrometer (Voyager DE-STR), 2) nanoLC-Q-TOF and 3) a nanoLC-LTQ-Orbitrap (ThermoElectron). The postdoc/tech will use these instruments for protein identification and protein-protein interaction studies, as well as (large scale) comparative proteomics using spectral counting and various stable isotope labeling techniques. Mass spectrometers and other proteomics and bioinformatics infrastructure are located within the van Wijk lab. The applicant MUST have a very strong background in mass spectrometry of peptides and proteins, as is evidenced by one or more publications in International Journals. Responsibilities and salary will be adjusted based on experience. Application Instructions: Send your CV and the names of 3 references to klaas van Wijk at [2]kv35@cornell.edu. Do not apply if you have no significant experience with Mass spectrometry. Recent Relevant papers from the van Wijk lab: Zybailov et al (2008) PlosOne 3(4); Majeran et al (2008) Molecular & Cellular Proteomics 9:1609-38.; Rutschow et al (2008) Plant Physiology 148(1):156-175; Sun et al (2009) NAR [3]37(Database issue):D969-974; Majeran et al (2009) Trends in Plant Science 14(2):100-109 Klaas J. van Wijk Associate Professor Director of Graduate Studies (DGS) for the Field of Plant Biology Dept of Plant Biology 332 Emerson Hall Cornell University Ithaca, NY 14853, USA tel +1-607-255-3664 [4]http://cbsu.tc.cornell.edu/vanwijk/ References 1. 3D"http://cbsu.tc.cornell.edu/vanwijk/" 2. 3D"mailto:kv35@Cornell.edu" 3. 3D"http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Sh 4. 3D"http://cbsu.tc.cornell.edu/vanwijk/" From galonyae from post.tau.ac.il Mon Apr 20 01:24:18 2009 From: galonyae from post.tau.ac.il (galonyae@post.tau.ac.il) Date: Mon Apr 20 11:22:14 2009 Subject: [Arabidopsis] total number of genes changed from specific stress for hypergeometric test Message-ID: <20090420092418.504445u058i9qvwy@webmail.tau.ac.il> Hi I want to calculate the p-value of the enriched list of genes (according various classifications, such as : cold, heat, uvb ext) from my microarray experiment, using hypergeometric test. For this calculation I need the total number of genes belonging to a specific group (e.g. cold) in a chip. Does anyone know where can I found how many genes are changed in each biotic or abiotic stress. for example: how many genes are changing in a chip as a result of cold stress (up and down regulated)? Thanks in advance, Yael From wenxian16 from yahoo.com Tue Apr 21 09:25:11 2009 From: wenxian16 from yahoo.com (Wenxian Sun) Date: Wed Apr 22 10:23:37 2009 Subject: [Arabidopsis] Questions on rice transformation Message-ID: <2573.9759.qm@web59203.mail.re1.yahoo.com> Hi all, ? I don't know if I will get? any responses about questions on rice study here.?Our lab attempted to do some rice transformation. But selection of rice transformants with kanamycin or Basta is not as good as Arabidopsis transformation. Is there any experts working on rice transformation here? I greatly appreciate it if you can tell me some tips on rice transformation with kanamycin or Basta selection. ? Best, ? Wenxian Sun From conferences from embl.de Wed Apr 22 11:45:07 2009 From: conferences from embl.de (EMBL Courses & Conferences) Date: Wed Apr 22 16:54:35 2009 Subject: [Arabidopsis] EMBO Conference Series on Morphogenesis and Dynamics of Multicellular Systems, EMBL Germany Message-ID: <200904221644.n3MGi7h15669@net.bio.net> EMBL Conference Announcement Dear Colleagues, We would like to announce a new EMBO Conference that we are organising from 2 - 6 October 2009 at the European Molecular Biology Laboratory, Heidelberg, Germany: EMBO Conference Series on Morphogenesis and Dynamics of Multicellular Systems The aim of this meeting is to bring together researchers addressing the role of collective cell behaviour in generating complex biological form. The number of researchers investigating such issues is rapidly increasing and we feel that there is a need to exchange knowledge in a specialised meeting. We are particularly keen to incorporate cutting-edge, imaging, biophysical and computational approaches that will drive the field in new directions. Registration and abstract deadline: 31 July 2009 Please check the conference website for more information: http://www.embl.de/conferences/morphogensis/2009 We have a reduced registration fee for PhD students. Please circulate this announcement to interested members and groups within your institute. The poster you can download at: http://www.embl.de/conferences/morphogenesis/2009/poster.pdf If you do not wish to receive further announcements of EMBL events, please reply to this message with "unsubscribe" in the subject line. ************************************************ Best wishes, Adela Valceanu Conference Officer European Molecular Biology Laboratory Meyerhofstr. 1 D-69117 Heidelberg Tel: +49-6221-387 8625 Fax: +49-6221-387 8158 For full event listings please visit our website: www.embl.org/events ************************************************ From jdfriesner from ucdavis.edu Wed Apr 22 11:44:34 2009 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Wed Apr 22 16:54:54 2009 Subject: [Arabidopsis] Posters due Next FRIDAY! ICAR- Scotland Message-ID: <200904221644.n3MGiYYu012800@musca.ucdavis.edu> Dear All The 20th International Conference on Arabidopsis Research will take place 30th June - 4th July 2009 in the historic and vibrant city of Edinburgh, Scotland, UK. http://arabidopsis2009.com/ Please note that the deadline for poster abstract submission closes on the 1st of May (next week). So if you would like to present a poster at the meeting submit your abstract NOW by visiting, http://www.arabidopsis.org/abstracts/ **************************************************************************** The keynote speaker at ICAR 2009 will be the Lasker Prize winning scientist Prof. David Baulcombe We have a great programme of world-renowned speaker confirmed including Enrico Coen, Caroline Dean, Joe Ecker, Jiri Firml, Nicholas Harberd, Alistair Hetherington, Andrew Millar, Ben Scheres, Mark Stitt and Jian-Kang Zhu. For a full programme visit http://arabidopsis2009.com/programme.html and http://arabidopsis2009.com/speakers.html The conference will consist of two plenary sessions each morning and four concurrent sessions each afternoon, which will include presentations chosen from submitted abstracts. The conference will feature a number of community-led workshops that will provide opportunities for focused discussion, whilst three poster sessions will provide ample time for research discussion. Delegates will also have a free afternoon between the Friday morning sessions and evening poster session to engage in networking, scientific discussion and to explore Edinburgh and it¹s surroundings. Important Deadlines Poster Abstract Submission Closes 1st May 2009 Regular Registration Closes 15th June 2009 We look forward to seeing you in Edinburgh. Ruth Bastow Joanna Friesner From conferences from embl.de Thu Apr 23 08:42:41 2009 From: conferences from embl.de (EMBL Courses & Conferences) Date: Fri Apr 24 00:25:06 2009 Subject: [Arabidopsis] EMBO Conference Series on Morphogenesis and Dynamics of Multicellular Systems, EMBL Germany Message-ID: <200904231342.n3NDghh27685@net.bio.net> Dear Colleagues, In our former e-mail - the link to our conference had a typing error. We are really sorry about this. Please see the corrected link to the conference: http://www.embl.de/conferences/morphogenesis/2009 Best wishes, Adela EMBL Conference Announcement Dear Colleagues, We would like to announce a new EMBO Conference that we are organising from 2 - 6 October 2009 at the European Molecular Biology Laboratory, Heidelberg, Germany: EMBO Conference Series on Morphogenesis and Dynamics of Multicellular Systems The aim of this meeting is to bring together researchers addressing the role of collective cell behaviour in generating complex biological form. The number of researchers investigating such issues is rapidly increasing and we feel that there is a need to exchange knowledge in a specialised meeting. We are particularly keen to incorporate cutting-edge, imaging, biophysical and computational approaches that will drive the field in new directions. Registration and abstract deadline: 31 July 2009 Please check the conference website for more information: http://www.embl.de/conferences/morphogenesis/2009 We have a reduced registration fee for PhD students. Please circulate this announcement to interested members and groups within your institute. The poster you can download at: http://www.embl.de/conferences/morphogenesis/2009/poster.pdf If you do not wish to receive further announcements of EMBL events, please reply to this message with "unsubscribe" in the subject line. ************************************************ Best wishes, Adela Valceanu Conference Officer European Molecular Biology Laboratory Meyerhofstr. 1 D-69117 Heidelberg Tel: +49-6221-387 8625 Fax: +49-6221-387 8158 For full event listings please visit our website: www.embl.org/events ************************************************ From verokeim from yahoo.com Fri Apr 24 02:49:08 2009 From: verokeim from yahoo.com (Veronica Keim) Date: Fri Apr 24 18:11:16 2009 Subject: [Arabidopsis] RNAi consulting Message-ID: <971964.8848.qm@web39502.mail.mud.yahoo.com> I?m working with an inducible interference RNA system in Arabidopsis thaliana,?which?is activated by the addition of 17 beta estradiol. I?ve read a few publications where mention?that this compound is supplied by Sigma Aldrich. My problem is that I wasn?t able to find the specific type of 17 beta estradiol that they use and?Sigma offer a lot of options. Does anybody know which kind of 17 beta estradiol should I use for this inducible system? Thank you and kind regards ____________________________________________________________________________________ ?Obt?n la mejor experiencia en la web! Descarga gratis el nuevo Internet Explorer 8. http://downloads.yahoo.com/ieak8/?l=e1 From tumer from AESOP.Rutgers.edu Mon Apr 27 10:59:29 2009 From: tumer from AESOP.Rutgers.edu (Nilgun Tumer) Date: Mon Apr 27 12:23:16 2009 Subject: [Arabidopsis] Postdoctoral Position at Rutgers University Message-ID: <01N8A60N69DQ9A6CZA@AESOP.Rutgers.edu> Postdoctoral Position at Rutgers University A postdoctoral position is available at the Biotech Center at Rutgers University in the laboratory of Dr. Nilgun E. Tumer to investigate the mechanisms of uptake, transport and cytotoxicity of ricin and Shiga-like toxins. The Tumer lab works on elucidating the basic mechanisms of action of ricin and Shiga-like toxins using Saccharomyces cerevisae and C. elegans as model systems (Biochemistry 2009, in press; Mol Microbiol. 2008, 70:1441; J. Biol. Chem. 2008, 283:6145). Both toxins depurinate the sarcin/ricin loop of the large rRNA and inhibit translation. Our goal is to identify the targets of these toxins to understand the mechanism by which they cause ribotoxic stress and cell death. The successful candidate will use genetic and cell biology approaches to investigate the mechanism of uptake and transport of the toxins in yeast and C. elegans and use chemical genomics approaches to identify the cellular pathways affected by these toxins. The ideal candidate should have a Ph.D. in a related field, experience with yeast and/or C.elegans genetics and cell biology and familiarity with chemical biology and bioinformatics. The Biotech Center is located in New Brunswick, New Jersey near New York City and Philadelphia and is equipped with state-of-the-art facilities and instrumentation. Rutgers University is an Equal Opportunity/Affirmative Action employer. Interested applicants should send their CV and names and addresses of three references to Dr. Nilgun E. Tumer (tumer@aesop.rutgers.edu). Information on our research can be found at: http://biotech.rutgers.edu/faculty/tumer.html, http://aesop.rutgers.edu/~plantbiopath/faculty/tumer/TUMER.HTML, http://lifesci.rutgers.edu/~molbiosci/faculty/tumer.html Contact: Nilgun Tumer, Foran Hall, Biotech Center, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901-8520. Tel: 732-932-8165 X215. From manoharanm from uapb.edu Wed Apr 29 10:24:55 2009 From: manoharanm from uapb.edu (Muthusamy Manoharan) Date: Wed Apr 29 10:44:39 2009 Subject: [Arabidopsis] Post-doctoral Research Associate Message-ID: Post-doctoral Research Associate Position: A Post-doctoral research associate position is available immediately to work on dry bean transformation. Qualifications: Candidate should hold a Ph.D. in Plant Science, Botany, Biology or a related discipline. Prior experience with tissue culture, transformation and molecular biology is desired. Responsibilities: Responsibilities include development of regeneration and transformation systems, construction of vectors (including for RNAi), genetic engineering of relevant genes, and molecular characterization of transgenic lines. The successful candidate must be able to independently design and conduct experiments, and willing to work on other projects in the laboratory. Salary: Commensurate with experience. Application: Applicant should submit CV, statement of research interests and the name and contact information of three references to: Dr. Muthusamy Manoharan Associate Professor Department of Agriculture University of Arkansas at Pine Bluff 1200 North University Dr. Pine Bluff, AR 71601 Phone: 870-575-8543 E-mail: manoharanm@uapb.edu Closing Date: May 31, 2009 or until suitable candidate is found. University of Arkansas at Pine Bluff is an equal opportunity/affirmative action institution. From jdfriesner from ucdavis.edu Wed Apr 29 12:27:31 2009 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Wed Apr 29 15:11:04 2009 Subject: [Arabidopsis] Last Chance Friday- poster abstract submission Message-ID: <200904291727.n3THRVVA013415@biglossa.ucdavis.edu> Friday May 1st is the last day to submit poster abstracts for the 20th International Conference on Arabidopsis Research taking place June 30th - July 4th 2009 in Edinburgh, Scotland. http://arabidopsis2009.com/ Joanna Friesner MASC/NAASC Coordinator UC Davis From articles.jpbcs from acadjourn.org Thu Apr 2 10:42:37 2009 From: articles.jpbcs from acadjourn.org (Journal of Plant Breeding and Crop Science) Date: Wed Sep 30 17:17:36 2009 Subject: [Arabidopsis] (no subject) Message-ID: <9f0c28b80904020842s5e8c9b8aqfcff343b29f21913@mail.gmail.com> *Journal of Plant Breeding and Crop Science* www.academicjournals.org/JPBCS * * Dear Colleague, The *Journal of Plant Breeding and Crop ** **Science** * *(JPBCS) *is a* *multidisciplinary peer-reviewed journal published monthly by Academic Journals ( www.academicjournals.org/JPBCS dedicated to increasing the depth of Crop Science across disciplines with the ultimate aim of improving plant research. *Call for Papers* *JPBCS* will cover all areas of plant breeding and crop science. The journal welcomes he submission of manuscripts that meet the general criteria of significance and scientific excellence, and will publish: ? Original articles in basic and applied research ? Case studies ? Critical reviews, surveys, opinions, commentaries and essays We invite you to submit your manuscript(s) to jpbcs@acadjourn.orgfor publication in the Maiden Issue (January 2009). Our objective is to inform authors of the decision on their manuscript(s) within four weeks of submission. Following acceptance, a paper will normally be published in the next issue. Instruction for authors and other details are available on our website; http://www.academicjournals.org/JPBCS/Instruction.htm JPBCS is an Open Access Journal One key request of researchers across the world is unrestricted access to research publications. Open access gives a worldwide audience larger than that of any subscription-based journal ad thus increases the visibility and impact of published work. It also enhances indexing, retrieval power and eliminates the need for permissions to reproduce and distribute content.JPBCS is fully committed to the Open Access Initiative and will provide free access to all articles as soon as they are published. Best regards, *Anighoro Clementina* Editorial Assistant Journal of Plant Breeding and Crop Science (JPBCS) E-mail: jpbcs@acadjourn.org, www.academicjournals.org/JPBCS From jpbcs from acadjourn.org Wed Apr 8 09:35:28 2009 From: jpbcs from acadjourn.org (Journal of Plant Breeding and Crop Science) Date: Wed Sep 30 17:17:51 2009 Subject: [Arabidopsis] (no subject) Message-ID: *Journal of Plant Breeding and Crop Science* www.academicjournals.org/JPBCS * * Dear Colleague, The *Journal of Plant Breeding and Crop ** **Science** * *(JPBCS) *is a* *multidisciplinary peer-reviewed journal published monthly by Academic Journals ( www.academicjournals.org/JPBCS dedicated to increasing the depth of Crop Science across disciplines with the ultimate aim of improving plant research. *Call for Papers* *JPBCS* will cover all areas of plant breeding and crop science. The journal welcomes he submission of manuscripts that meet the general criteria of significance and scientific excellence, and will publish: ? Original articles in basic and applied research ? Case studies ? Critical reviews, surveys, opinions, commentaries and essays We invite you to submit your manuscript(s) to jpbcs@acadjourn.orgfor publication in the Maiden Issue (January 2009). Our objective is to inform authors of the decision on their manuscript(s) within four weeks of submission. Following acceptance, a paper will normally be published in the next issue. Instruction for authors and other details are available on our website; http://www.academicjournals.org/JPBCS/Instruction.htm JPBCS is an Open Access Journal One key request of researchers across the world is unrestricted access to research publications. Open access gives a worldwide audience larger than that of any subscription-based journal ad thus increases the visibility and impact of published work. It also enhances indexing, retrieval power and eliminates the need for permissions to reproduce and distribute content.JPBCS is fully committed to the Open Access Initiative and will provide free access to all articles as soon as they are published. Best regards, *Anighoro Clementina* Editorial Assistant Journal of Plant Breeding and Crop Science (JPBCS) E-mail: jpbcs@acadjourn.org, www.academicjournals.org/JPBCS From petre from iaria.org Fri Apr 10 10:52:54 2009 From: petre from iaria.org (Petre Dini) Date: Wed Sep 30 17:18:06 2009 Subject: [Arabidopsis] (no subject) Message-ID: <49DF6A68.2030407@iaria.org> Invitation, We are in the process of inviting the program committee members for the BioSciencesWorld 2009, including *BIOTECHNO 2009, The Second International Conference on Advances in Biotechnologies** * *http://www.iaria.org/conferences2009/BIOTECHNO09.html* * * *BIOINFO 2009, The First International Conference on Advances in Bioinformatics and Applications** * http://www.iaria.org/conferences2009/BIOINFO09.html * * *BIOSYSCOM 2009, The First International Conference on Computational and Systems Biology and Microbiology** * http://www.iaria.org/conferences2009/BIOSYSCOM09.html * * *BIOGREEN 2009, The First International Conference on Advances in Renewable and Sustainable Energies** * http://www.iaria.org/conferences2009/BIOGREEN09.html * * *BIODIV 2009, The First International Conference on Biodiversity and Invasion Control** * http://www.iaria.org/conferences2009/BIODIV09.html *BIOENVIRONMENT 2009, The First International Conference on Environmental Change Awareness** * http://www.iaria.org/conferences2009/BIOENVIRONMENT09.html We would greatly appreciate you accept our invitation to join one or more technical program committees. BioSciencesWorld 2009 is scheduled in Cancun, November 15-20, 2009. We count on program committee members to submit papers (hopefully attend), help reviewing 2-3 papers, recommending new program committee members, suggesting topics improvements, and disseminating the Call for Papers. Please reply to the invitation to petre@iaria.org with - your most up to date affiliation and - 1-2 conferences, and your topics of interest, e.g., BIOINFO/Chemoinformatics, BIODIV/Bioinvasion We would like to post the committee as soon as possible and start distributing the Call for Papers. Thanks for your potential help, IARIA Committees Board Chair --------------------------- Prof. Dr. Petre Dini ---------------------------