From donito from woh.rr.com Tue Mar 4 20:38:54 2008 From: donito from woh.rr.com (Don Shuff) Date: Wed Mar 5 09:15:11 2008 Subject: [Drosophila] Double sex symbols Message-ID: <20080304203854.468487f2.donito@woh.rr.com> I've recently come across the use of double male or double female symbols within the diagrams of various Drosophila crosses. Can someone explain to me the purpose of using two male or female symbols next to each other? Thank you. Don From kcook from bio.indiana.edu Wed Mar 5 09:20:12 2008 From: kcook from bio.indiana.edu (Kevin Cook) Date: Wed Mar 5 09:20:01 2008 Subject: [Drosophila] Double sex symbols Message-ID: <200803051418.m25EI1Z4000549@mail-relay.iu.edu> It's plural. One symbol for one fly, two for more than one. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@bio.indiana.edu From jim.goodchild from syngenta.com Tue Mar 11 06:54:25 2008 From: jim.goodchild from syngenta.com (jim.goodchild@syngenta.com) Date: Tue Mar 11 08:25:46 2008 Subject: [Drosophila] Job vacancy at Syngenta, Berkshire, UK Message-ID: <4000A5449019CD40A81E2513E34F6A810114D6A1@gbjhmsxmb01.EAME.SYNGENTA.ORG> Dear all, I work for Syngenta at Jealott's Hill Research Station, Berkshire, UK where I lead a team using chemical genetics and electrophysiology approaches to understanding the mode of action of insecticides and mechanisms of insecticide resistance. I currently have a vacancy for a 12 month temporary position (laboratory based) in my team. A brief description of the role is below. If anyone would like to know more they can e-mail me at jim.goodchild@syngenta.com. Interested applicants should send a CV accompanied by a short description of their background and interest. Syngenta is a world-leading agribusiness committed to sustainable agriculture through innovative research and technology. Understanding the mode of action of insecticidal compounds is a key issue in the development of new crop protection products. Genetic and biochemical assays allow us to answer critical questions about the way small molecules act on insect pests. We are looking to recruit a senior experimentalist for twelve months, working in the Invertebrate Genetics and Physiology team within the Bioscience Dept based at Jealott's Hill International Research Centre near Bracknell in the UK. The job holder will be required to deliver high quality data to tight deadlines, to rapidly learn new tasks and have a creative and scientific approach to problem solving. They will be required to work flexibly across projects, to determine or monitor modes of action or establish the intrinsic potencies of new lead chemicals. This may involve the application of forward genetic approaches in Drosophila to the study of pathophysiological response to insecticide treatment or to the understanding of the basis of insecticide resistance. The work may involve comparing behavioural, symptomological or electrophysiological responses in wild-type and mutant backgrounds. Laboratory experience in any of the following areas would be of value but is not essential; model organism genetics, phenotypic analysis, bioinformatics, studies of behaviour or symptomology, patch-clamping, neuronal or neuromuscular recording, microinjection, sterile-technique, cell or tissue culture, assaying of calcium-flux or membrane potential using fluorescent dyes, confocal microscopy and imaging. Candidates will have postgraduate qualifications and/or practical experience in biological or biochemical research. The candidate must be computer literate and capable of working both independently and as part of a team. Good organisation skills and attention to detail are required. He or she will have excellent written and oral communication skills. Full training will be given on all aspects of safe procedure. Jim Goodchild e-mail: jim.goodchild@syngenta.com www.syngenta.com Syngenta Limited, Registered in England No 2710846 Registered Office : Syngenta Limited, European Regional Centre, Priestley Road, Surrey Research Park, Guildford, Surrey, GU2 7YH, United Kingdom From andre_faustino from hotmail.com Tue Mar 11 08:49:41 2008 From: andre_faustino from hotmail.com (Andre Faustino) Date: Tue Mar 11 09:13:03 2008 Subject: [Drosophila] Research Position Message-ID: RESEARCH POSITION Gene PreDiT is a young biotech start-up company located in Biocant Park (Cantanhede, Portugal) that is now starting its activity. We are looking for a highly skilled, dynamic and motivated researcher, with an interest in the entrepreneurial field. We welcome individuals with a Master or PhD Degree in Life Sciences or Pharmacology, preferentially with a background and work experience with Drosophila melanogaster. Applications should be sent to: Gene PreDiT, SA Biocant Park - Parque Tecnol?gico de Cantanhede N?cleo 04, Lote 02 3060-197 Cantanhede or to genepredit@genepredit.com.pt _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 From k.hahn from dkfz-heidelberg.de Fri Mar 14 07:07:14 2008 From: k.hahn from dkfz-heidelberg.de (Hahn Katrin) Date: Fri Mar 14 08:36:17 2008 Subject: [Drosophila] monoclonal S2 cell population Message-ID: <61664492828BA141A2A9FAA8BBFF21571B7059@dkfzex2.ad.dkfz-heidelberg.de> Hi, I wonder whether anybody can give me some advice how to make a monoclonal cell line from S2 cells. I have polyclonal S2 cells stably expressing the protein of interst, but since the expression levels are so heterogenous I need to get a monoclonal population. Thank you for your help! From dmerrill from k-state.edu Thu Mar 13 09:43:23 2008 From: dmerrill from k-state.edu (Arthropod Genomics) Date: Fri Mar 14 08:36:41 2008 Subject: [Drosophila] Hotel Deadline-3/20; Posters&Registration continue to be accepted Message-ID: <003201c88518$96f7ca30$78738281@ECOGENOFFICE> You can still submit a poster abstract and register to attend the 2ND ANNUAL ARTHROPOD GENOMICS SYMPOSIUM: "New Insights from Arthropod Genomics" April 11-13, 2008, Muehlbach Hotel (operated by Marriott) Downtown Kansas City The room block at the Muehlbach/Marriott hotel will expire 3/20/08 (or prior to 3/20 if the block sells out); so make your hotel reservations TODAY! A link for making reservations at the discounted room rate can be found at www.ksu.edu/agc/symposium.shtml or telephone 1-800-228-9290. The deadline to submit POSTER ABSTRACTS for presentation during two poster sessions has been extended!!! We welcome your presentation of a poster at the Symposium. Abstract Guidelines can be found at www.ksu.edu/agc/symposium.shtml. REGISTRATION continues to be open! Online registration, a schedule, brochure and additional information are available at www.ksu.edu/agc/symposium.shtml. SYMPOSIUM WEBSITE: www.k-state.edu/agc/symposium.shtml SYMPOSIUM PROGRAM: The main symposium sessions will take place on Friday-Saturday, April 11-12. Speakers will present new insights from genomic approaches in arthropods and describe the development of tools for genomic analysis. Optional workshops are scheduled for Thursday and Friday evenings. An evening of jazz and KC barbeque is planned for Saturday night. On Sunday morning, participate in a roundtable discussion with the ArthropodBase Consortium. Activities will conclude by noon on Sunday. FEATURED SPEAKERS: **John Kenneth Colbourne, Indiana University, Preservation, expansion and invention of crustacean genes with reference to insect genomes. **Christine G. Elsik, Georgetown University, Unusual base composition of the honey bee genome. **Sarjeet S. Gill, University of California, Riverside, Mosquito midgut interactions with bacterial toxins. **Catherine A. Hill, Purdue University, Tick genome organization and evolution. **Thomas Kaufman, Indiana University, The latest news from CNN: What the 12 sequenced Drosophila genomes have told us about rapidly evolving genes and positive selection. **J. Robert Manak, University of Iowa, Empirical annotation of arthropod genomes using tiled genomic microarrays. **Subbaratnam Muthukrishnan, Kansas State University, Functional genomics of insect chitin metabolism. **Hugh M. Robertson, University of Illinois at Urbana-Champaign, What we've learned about the insect chemoreceptors from arthropod genome projects. **Bruce R. Schatz, University of Illinois at Urbana-Champaign, BeeSpace: Interactive functional analysis of arthropod genomic data. **Jeff Stuart, Purdue University, Avirulence, sex determination, and a physical map of the Hessian fly genome. **Judy Willis, University of Georgia, Insect cuticular proteins: Annotation, proteomics, expression, evolution. **Evgeny Zdobnov, University of Geneva, Medical School, Switzerland, A comparative perspective on insect genomes. KANSAS CITY JAZZ AND BARBEQUE: Participants are encouraged to stay Saturday night for an optional evening of jazz and KC barbeque in the historic 18th and Vine district. WORKSHOPS AND ROUNDTABLE DISCUSSION: On Thursday evening, a workshop on "Community Contributions to Genome Annotation" will feature a presentation on use of the Apollo Genome Annotation Curation Tool by Dr. Chris Elsik (BeeBase). On Friday evening, Dave Clements (NESCent) and Scott Cain (CSHL) will lead a workshop, "Chado Databases and Integration with GMOD Tools." Throughout the meeting, arthropod genome database and bioinformatics tool developers will be available for individual training on Apollo and database use as well as BeeSpace, the automated literature annotation system developed by Bruce Schatz and colleagues. On Sunday morning, participate in a roundtable discussion led by members of the ArthropodBase Consortium regarding the generation of integrated arthropod genome databases and tools for genome analysis, and community curation. Symposium attendees are invited to participate in these additional events. REGISTRATION: The registration fee of $325 ($200 for graduate and undergraduate students), includes a welcome reception Thursday evening, breakfast and lunch on Friday and Saturday, and light refreshments at the Friday poster session. Additional fees apply for the Apollo Workshop Thursday evening and Saturday night dinner. We look forward to seeing you in Kansas City! Please share this announcement with colleagues and students! Susan J. Brown, Professor Director, Center for Genomic Studies on Arthropods Affecting Human, Animal and Plant Health by Doris Merrill, Program Coordinator K-State Arthropod Genomics Center Division of Biology, Kansas State University 116 Ackert Hall, Manhattan, KS 66506-4901 (785) 532-3482, dmerrill@k-state.edu www.k-state.edu/agc From A.Schenck from antrg.umcn.nl Thu Mar 13 20:35:59 2008 From: A.Schenck from antrg.umcn.nl (A.Schenck@antrg.umcn.nl) Date: Fri Mar 14 08:37:12 2008 Subject: [Drosophila] PhD position Message-ID: <60A99F587D3A054EB33F75B7579C1AFD4F5562@UMCEXBE02.umcn.nl> Dear all, I have an available Ph.D. position. Please forward the ad to students that may be interested (thanks!): Ph.D. position (4 years) available in the Department of Human Genetics at the Nijmegen Centre of Molecular Life Science (NCMLS), Radboud University Nijmegen Medical Centre (Nijmegen, the Netherlands) We are currently seeking a Ph.D. fellow for the research group of Dr. Annette Schenck. The successful applicant will join a young, highly motivated and interdisciplinary team focusing on functional studies of disease genes implicated in mental retardation. The group uses the fruitfly Drosophila melanogaster as a model organism to dissect molecular networks and mechanisms underlying neuronal connectivity, function and behaviour. It operates in close association with the Human Genetics Lab of Dr. Hans van Bokhoven who continuously identifies novel genes implicated in brain diseases. Enthusiastic, creative and interactive individuals with a background in genetics, molecular, cell, developmental and/or neurobiology and a MSc/diploma in life sciences are encouraged to apply. Previous experience with Drosophila or other model organisms would be advantageous, but is not obligatory. Nijmegen is the Netherlands' oldest city. It offers historic sites and museums, borders beautiful forest countryside and polder landscapes and hosts one of the Netherland's most successful universities. The NCMLS, endowed with state-of-the-art equipment and core facilities, is amongst the leading European centres of biomedical research. Further information on our research and research environment can be found at the institute's websites: http://www.ncmls.nl/NCMLS/MenuStructures/PI/theme3/AnnetteSchenck.asp and www.ncmls.eu . The operating language of the Institute is English. Please send your application (letter of motivation, complete curriculum vitae) and at least two reference letters before April 15th to: Dr. Annette Schenck, E-mail: a.schenck@antrg.umcn.nl ************************************************************************ ******* Annette Schenck, PhD Group leader, Department of Human Genetics (855) Nijmegen Centre for Molecular Life Science (NCMLS), Radboud University Nijmegen Medical Center Geert Grooteplein 10 6525 GA Nijmegen, the Netherlands Tel: +31 24 3617431/3614017 (secretary), Fax: +31 24 3668752 mailto: a.schenck@antrg.umcn.nl http://www.ncmls.nl/NCMLS/MenuStructures/PI/theme3/AnnetteSchenck.asp ************************************************************************ ******* Het UMC St Radboud staat geregistreerd bij de Kamer van Koophandel in het handelsregister onder nummer 41055629. The Radboud University Nijmegen Medical Centre is listed in the Commercial Register of the Chamber of Commerce under file number 41055629. From sa08383 from wotan.mdacc.tmc.edu Tue Mar 18 12:04:10 2008 From: sa08383 from wotan.mdacc.tmc.edu (Kathleen Gajewski) Date: Tue Mar 18 12:13:47 2008 Subject: [Drosophila] Protein Extract from Larvae In-Reply-To: <47C1EB7F.5D69.009D.0@ScrippsCollege.edu> References: <47C1EB7F.5D69.009D.0@ScrippsCollege.edu> Message-ID: Ivy, Have you tried freeze-drying? I've done Westerns, 2D gels and RNA preps from freeze tried tissues with good results. Here's the reference: Fujita S.C., Inoue H., Yoshioka T., Hotta Y., 1987. Quantitative tissue isolation from Drosophila freeze-dried in acetone. Biochem. J. 243, 97-104. Good luck, KG On Feb 25, 2008, at 12:11 AM, Ivy McDaniel wrote: > Hello Drosophila Community, > > I'm looking for a good protocol, or even just general tips for making > protein extracts from third-instar larvae and adult flies. I'm dealing > with a very large protein, and I'm having a lot of trouble avoiding > degradation. Grinding adults/larvae/salivary glands directly in > boiling SDS loading buffer hasn't worked, nor has one protocol where I > included protease inhibitors at every step. Any advice at all would be > greatly appreciated! > > Thanks, > Ivy > > > _______________________________________________ > Dros mailing list > Dros@net.bio.net > http://www.bio.net/biomail/listinfo/dros From don.shuff from gmail.com Wed Mar 26 08:27:19 2008 From: don.shuff from gmail.com (donito) Date: Wed Mar 26 09:00:12 2008 Subject: [Drosophila] Looking for stocks of Antp Message-ID: <75aacdbb-4142-4f0f-8d18-d75f002359dc@m36g2000hse.googlegroups.com> Anyone know where I can find a stock of Antp[Yu] and an Antp mutant under heat stock control. Thank you. Don