From Heather.Vincent from manchester.ac.uk Thu Jan 1 05:24:12 2009 From: Heather.Vincent from manchester.ac.uk (Heather Vincent) Date: Thu Jan 1 12:00:55 2009 Subject: [Immunology] Introduction to Bioinformatics, March 2009 Message-ID: <495C99CC.6010405@manchester.ac.uk> Introduction to Bioinformatics, from The University of Manchester, is just one of a number of online courses in bioinformatics, immunology and immunogenetics. The individual courses are suitable for anyone with an interest in immunological bioinformatics or systems immunology. The credits can also count towards either the MSc in Bioinformatics or the MSc in Immunology and Immunogenetics (http://www.ls.manchester.ac.uk/postgraduate/distancelearning/). The course is delivered in a Virtual Learning Environment, which allows us to extend the classroom into the web. Teaching and learning are focussed around tutor-supported exercises. In this course, participants work together on the interpretation of their results, before receiving feedback from the course tutor. Participants who wish to be assessed for credits at MSc level should complete additional independent research. Week 1 Introduction to distance learning Bioinformatics as a knowledge-based discipline Weeks 2 and 3 Introduction to the sequence databases Quality, redundancy and annotation Sequence retrieval exercise and group discussion Weeks 4 and 5 Nucleic acid sequence analysis 1. locating features in a genomic sequence; 2. resources for genome structure prediction; 3. evaluating the programs; 4. genome annotation. Group work on the annotation of a genomic sequence Weeks 6 and 7 Independent research and assessment Weeks 8 and 9 Sequence alignment and database search 1. use of BLAST and FASTA, choosing suitable options; 2. interpretation of the results. Weeks 10 and 11 Protein function prediction. 1. multiple sequence alignment; 2. prediction of : a) transmembrane helices; b) other hydrophobic regions; c) various signal sequences. 3. use of the motif and profile databases; Weeks 12 and 13 Protein structure prediction Practical exercise and group discussion Weeks 14 to 16 Independent research and work on the second assessment Additional information is available here : http://octette.cs.man.ac.uk/bioinformatics/modules/bs6061.html or from Heather.Vincent from manchester.ac.uk From jing.wei from helsinki.fi Wed Jan 14 19:20:25 2009 From: jing.wei from helsinki.fi (Jing Wei) Date: Wed Jan 14 20:05:05 2009 Subject: [Immunology] something about MACS Message-ID: <20090115022025.19075oje6y92cm6h@webmail.helsinki.fi> could you help with this problem? I am doing MACS now and the result is not very good. Do you have good result with MACS on cell proliferation? Does the beads have some effect on the cells. In my experiment the cell will pass the colums 5 times will this do harm to the cell? Thank you very much and it will be a great help for me. Best regards, Jing Wei, B.Sc Petri Salven laboratory Institute of Biomedicine/ Center of Excellence in Cancer Biology Biomedicum, 5th floor (B509b) P.O.B 63 00014 University of Helsinki FINLAND email: jing.wei@helsinki.fi phone: +358919125385 From biospace from noster-it.com Thu Jan 15 10:37:08 2009 From: biospace from noster-it.com (biospace) Date: Thu Jan 15 11:55:05 2009 Subject: [Immunology] Protein Identified That Turns Off HIV-fighting T Cells Message-ID: <82388061-2f75-4dc3-ba79-cfd8b0383786@u18g2000pro.googlegroups.com> In HIV-infected patients the body's immune system is unable to fight off the virus. A new study to be published online on November 10th in the Journal of Experimental Medicine shows that T cells in HIV- infected individuals express a protein called TIM-3, which inactivates their virus killing capacity. Blocking this protein, the study suggests, might one day help patients to eliminate HIV as well as other chronic infections. Large numbers of virus-fighting T cells can be found in the blood of most chronically infected HIV patients. However these cells eventually become exhausted and cannot function. To identify the cause of this exhaustion, a team of researchers at the University of Toronto, lead by Mario Ostrowski, compared blood from healthy individuals and HIV patients. In the patients, TIM-3 was found on a large number of HIV- specific T cells, and the number of TIM-3-positive cells increased with the severity of infection. Under normal circumstances, exposing T cells to bits of virus causes the cells to replicate and produce virus-killing chemicals. Cells expressing TIM-3, however, were unreactive and TIM-3 was to blame; disrupting its signals restored the cells' virus-fighting functions. TIM-3 normally gets expressed on T cells after they carry out their normal function, perhaps as a way to turn the cells off and thus prevent excessive inflammation. But during HIV infection, persistent TIM-3 expression may help the virus avoid T cell attack. Tonny -------------- More bio-med news & videos Portal to share biological information-data between people http://biospace.ethz.ch From ashis.rasaily from gmail.com Fri Jan 16 22:52:12 2009 From: ashis.rasaily from gmail.com (ashis) Date: Fri Jan 16 23:23:18 2009 Subject: [Immunology] Re: NEED HELP References: Message-ID: <26d6cd1b-335b-4c04-b1a3-5c949da4398e@z27g2000prd.googlegroups.com> hi, could you tell me a good univ to do phd in immunology??i would be very grateful.thank you ashis From harjitsinghvet from gmail.com Thu Jan 22 07:09:54 2009 From: harjitsinghvet from gmail.com (khalsa meri jaan ki jaan) Date: Thu Jan 22 12:33:55 2009 Subject: [Immunology] NEED HELP " PROTOCOL FOR GUINEA PIG IgG2a ASSAY" Message-ID: I NEED ELISA PROTOCOL FOR IgG2a ESTIMATION TO ASSAY TH1 RESPONSE IN GUINEA PIG. IF ANYONE CAN HELP ME I WILL BE GRATEFUL. THANKING YOU IN ADVANCE DR.HARJEET SINGH BANGALORE INDIA From dkantar from assumption.edu Mon Jan 26 16:30:18 2009 From: dkantar from assumption.edu (Kantarelis, Demetrius (Economics)) Date: Mon Jan 26 17:14:13 2009 Subject: [Immunology] Hawaii 2009 Immunology Conference References: Message-ID: Hawaii / USA 2009 Immunology Conference CALL FOR ABSTRACTS / PAPERS / POSTERS: The Frontiers in Immunology Research 2009 Conference will be held in Hawaii (Kona), USA (July 22-26 at the Sheraton Hotel.) You may participate as panel organizer, presenter of one or two papers or posters or as an observer. The deadline for abstract / poster submission and registration is: March 30th, 2009. For more information, please contact the Frontiers in Immunology Research Network (FIRN) as follows: FIRN, 64 Holden Street, Worcester, MA 01605-3109, USA. Telephone: 508-852-3937, Fax: 508-595-0089, Email: