From mbarlett from indiana.edu Wed Mar 5 12:56:32 2008 From: mbarlett from indiana.edu (Barlett, Melissa A.) Date: Wed Mar 5 18:53:43 2008 Subject: [Microbiology] Agrobacterium Message-ID: <80897EE7FC527E45A17655330E2BDDC80ED4A44FBE@iu-mssg-mbx04.ads.iu.edu> Hello, I am working with Agrobacterium tumefaciens in a low-oxygen environment, and I have been examining the literature for some general information about A. tumefaciens ecology, and metabolism, and possible adaptations to an oxygen-limiting environment and having trouble. Most of the information on Agro is about plant interactions, transformation, or the Ti plasmid. If anyone knows of some good resources for general non-plant interacting Agro, that would be appreciated. I'm trying to cover all my bases to be sure I haven't missed a key piece of literature. Thank you! Melissa Barlett Picardal Lab School of Public and Environmental Affairs Indiana University Bloomington From mbarlett from indiana.edu Wed Mar 5 16:48:13 2008 From: mbarlett from indiana.edu (Barlett, Melissa A.) Date: Wed Mar 5 18:53:59 2008 Subject: [Microbiology] Agrobacterium pt. 2 Message-ID: <80897EE7FC527E45A17655330E2BDDC80ED4A44FBF@iu-mssg-mbx04.ads.iu.edu> Hello again, As an addendum to my previous request about Agrobacterium, is anyone aware of Agro, or any bacterium actually, that when grown on acetate as the sole carbon source, converts the acetate to glucose (and possibly further to cellulose)? Essentially, I grow my A. tumefaciens in acetate-containing minimal media, and I'm trying to determine if it is likely or impossible that fibers that I see using SEM are cellulose or something else. I know Agro produces cellulose, but it appears to require various glucose precursors, so I'm trying to determine if they would be made from acetate, or if I should successfully have cellulose-free cells. Melissa Barlett Picardal Lab School of Public and Environmental Affairs Indiana University Bloomington From kparisian from ci.great-falls.mt.us Fri Mar 7 09:57:07 2008 From: kparisian from ci.great-falls.mt.us (Kevin Parisian) Date: Fri Mar 7 15:45:48 2008 Subject: [Microbiology] Anti-bacterial additives for anaerobic thiobaccillus Message-ID: <67136C4668A1D643B42EF788147A1D76A644CB@SRVHALE.cogf.lan> Hello Did you ever develop a polymeric coating for wastewater structures and sanitation systems to prevent corrosion due to micro bacterial action? Sincerely, Kevin Parisian, Civil Engineer City of Great Falls - Engineering PO Box 5021 1025 25th Avenue North East Great Falls, Montana 59403 (406) 771-1258 (406) 771-0700 fax From wayne.wurtsbaugh from usu.edu Tue Mar 11 11:52:39 2008 From: wayne.wurtsbaugh from usu.edu (Wayne Wurtsbaugh) Date: Tue Mar 11 13:22:59 2008 Subject: [Microbiology] 10th International Conference on Salt Lake Research - Abstract deadline 13 March Message-ID: <47D6B8D7.3040403@usu.edu> Dear Dr. Simon; I would appreciate it if you could advise the halophile group that you work with about the salt lake conference in May. We've extended the abstract deadline until Thursday (March 13th), and I would really like to see additional contributions from the microbiologists working on hypersaline environments. I've attached the 3rd circular, but for information it is probably best to go directly to the conference web site: http://www.isslr.org/gsl2008/in/ Sincerely, Wayne Wurtsbaugh -- Wayne A. Wurtsbaugh, Professor Watershed Sciences Department/Ecology Center Utah State Univ., Logan, UT 84322-5210, USA. 435 797-2584 (work); 435 797-1871 (FAX); www.cnr.usu.edu/faculty/wayne-wurtsbaugh 'The most exciting phrase to hear in science, the one that heralds new discoveries, is not "Eureka!" (I've found it!), but "That's funny..." --Isaac Isimov From gifiamma from jumpy.it Sun Mar 16 05:33:49 2008 From: gifiamma from jumpy.it (paolo pelini) Date: Sun Mar 16 12:39:36 2008 Subject: [Microbiology] research for paolo pelini cancer pulmonary by bacteria pseudomonas Message-ID: PAOLO PELINI: I lung cancer is caused by certain bacteria Pseudomonas, which I identified. These bacteria cause a mutation in the DNA deletion ie loss of one or more pairs of bases, by certain enzymes produced by these bacteria. This involves a 'alteration or' inactivation of certain genes (FHIT gene, rb gene, P53 gene), which changes or inactivation of these genes lead to the lack of training of some regulatory proteins or training of these proteins wrong then the bacterium releases its genes which lead to the formation of lung cancer cells. This theory is supported by the presence, the level of cancer cells, leukocytes, which accrediting the presence of these bacteria that cause lung cancer. This explains why more than some people who smoke or be in contact with carcinogens cancer does not arise, this is due to the immune response that is the ability of the immune system to respond to bacterial infection. For more information about the complete theory visit Http://www.tumori-batteri.info The theory is on the site: Http://www.tumori-batteri.info New Group Http://groups.google.it/group/tumore-batteri?hl=it PAOLO PELINI: Secondo me il tumore polmonare ? causato da alcuni batteri pseudomonas, da me individuati. Questi batteri causano una mutazione del DNA per delezione cio? per perdita di una o pi? coppie di basi, ad opera di alcuni enzimi, prodotti da questi batteri. Ci? comporta un' alterazione o un' inattivazione di alcuni geni oncosopressori (gene FHIT,gene rb,gene P53); le quali alterazioni o l'inattivazioni di questi geni portano alla mancata formazione di alcune proteine regolatrici o alla formazione errata di queste proteine successivamente il batterio rilascia dei propri geni i quali portano alla formazione di cellule tumorali polmonari. Questa teoria ? favorita anche dalla presenza, al livello delle cellule cancerogene, di leucociti, i quali accreditano la presenza di questi batteri che causano il tumore polmonare. Ci? spiega in oltre il perch? in alcune persone che fumano o vanno a contatto con sostanze cancerogene il tumore non si manifesti, ci? ? dovuto alla risposta immunitaria ci? ? alla capacita del sistema immunitario di rispondere all infezione batterica. Per ulteriori informazioni e per la teoria completa visitate il sito http://www.tumori-batteri.info la teoria ? presente sul sito: http://www.tumori-batteri.info new group http://groups.google.it/group/tumore-batteri?hl=it From abc from tiscali.it Sun Mar 16 06:44:50 2008 From: abc from tiscali.it (menzasarma) Date: Sun Mar 16 12:39:40 2008 Subject: [Microbiology] Re: tumore al polmone causato da batteri pseudomonas In-Reply-To: <8f3fc03c-e81e-4b24-b504-4aadbb6aa760@p25g2000hsf.googlegroups.com> References: <8f3fc03c-e81e-4b24-b504-4aadbb6aa760@p25g2000hsf.googlegroups.com> Message-ID: <47dd0833$0$16037$5fc30a8@news.tiscali.it> paolo pelini ha scritto: > di paolo pelini > PAOLO PELINI: Secondo me il tumore polmonare ? causato da alcuni > batteri pseudomonas, da me individuati. > Questi batteri causano una mutazione del DNA per delezione cio? per > perdita di una o pi? coppie di basi, ad opera di alcuni enzimi, > prodotti da questi batteri. Ci? comporta un' alterazione o un' > inattivazione di alcuni geni oncosopressori (gene FHIT,gene rb,gene > P53); le quali alterazioni o l'inattivazioni di questi geni portano > alla mancata formazione di alcune proteine regolatrici o alla > formazione errata di queste proteine successivamente il batterio > rilascia dei propri geni i quali portano alla formazione di cellule > tumorali polmonari. > Questa teoria ? favorita anche dalla presenza, al livello delle > cellule cancerogene, di leucociti, i quali accreditano la presenza di > questi batteri che causano il tumore polmonare. Ci? spiega in oltre il > perch? in alcune persone che fumano o vanno a contatto con sostanze > cancerogene il tumore non si manifesti, ci? ? dovuto alla risposta > immunitaria ci? ? alla capacita del sistema immunitario di rispondere > all infezione batterica. Per ulteriori informazioni e per la teoria > completa visitate il sito > http://www.tumori-batteri.info > > la teoria ? presente sul sito: > http://www.tumori-batteri.info > new group > http://groups.google.it/group/tumore-batteri?hl=it Ti ripeto una domanda che ti ho gi? fatto tempo fa e a cui non hai risposto: hai isolato lo pseudomonas da tessuti tumorali? Se la risposta ? no, trova una spiegazione pi? plausibile. -- L'umanit? ? divisa in due categorie: i geni e quelli che dicono di esserlo. Io sono un genio. (Groucho Marx) Se vuoi scrivermi, prendi carta penna e calamaio. In alternatiVa, menzasarmaATgmail.com From marielgullian from mac.com Sun Mar 16 13:47:03 2008 From: marielgullian from mac.com (Mariel Gullian) Date: Mon Mar 17 12:00:19 2008 Subject: [Microbiology] Pathogen Modeling Program 5.0 Message-ID: <507E7A5A-FB56-4AF6-A651-82C5695B324C@mac.com> How can I get the pathogen modeling program. Thank you for the information. Regards Mariel Gullian From yjgent from nospamcox.net Mon Mar 17 19:17:06 2008 From: yjgent from nospamcox.net (John Gentile) Date: Tue Mar 18 10:09:22 2008 Subject: [Microbiology] Re: Pathogen Modeling Program 5.0 References: Message-ID: <2008031720170616807-yjgent@nospamcoxnet> On 2008-03-16 14:47:03 -0400, Mariel Gullian said: > How can I get the pathogen modeling program. > > Thank you for the information. > > Regards > Mariel Gullian Have you tried a Google search? I found it in about 10 seconds, and it appears to be up to version 7, and there is also an online version. Look at the system requirments, it looks like a Windows only version for download, you might have to use the online version if you are using a Mac. Here is the web site: http://www.arserrc.gov/mfs/PMP7_start.htm -- John Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net From bactitech from nospamhortonsbay.com Wed Mar 19 22:31:34 2008 From: bactitech from nospamhortonsbay.com (JEDilworth) Date: Thu Mar 20 13:41:52 2008 Subject: [Microbiology] Re: Pathogen Modeling Program 5.0 References: <2008031720170616807-yjgent@nospamcoxnet> Message-ID: It amazes me how many people post on these groups and seem clueless about search engines. Mariel - there is Google, Yahoo, Dogpile (search engine of search engines) and many others. You just put in word or words and press search. Then you start to look and read. If you don't find what you want you put in variations of your search and try again. http://www.google.com/search?hl=en&q=Search+Engine - a Google search of "Search Engine" (interestingly, Alta Vista comes up first on Google. Is that weird or what?) They really do work. Thanks, John :-). Judy Dilworth, M.T. (ASCP) Microbiology > Have you tried a Google search? I found it in about 10 seconds..... From sandro_photo from yahoo.com Mon Mar 24 01:54:27 2008 From: sandro_photo from yahoo.com (Mad Twins Dad) Date: Mon Mar 24 12:39:04 2008 Subject: [Microbiology] Problem with cultivation on anaerobic solid media In-Reply-To: <20071114195842.M68778@louisiana.edu> References: <20071114195842.M68778@louisiana.edu> Message-ID: <16246239.post@talk.nabble.com> Luzan Tatiana wrote: > > In my lab we use hungate bottles only for the liquid anaerobic media and > bi- > layer solid media. We have to do inoculations on solid meida only using > Petri dishes in the anaerobic jar supplemented with gas-packs or > controlled > atmosphere anaerobic chamber. THere is a problem in the chamber: the cag > mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While > incubating plates inside, the media dries up very quickly and an enourmous > condensation is forming! IT is almost a lake of water there. > > IS there any product which would absorb an acess condensate? > Thanks a lot > > -- > > Tanya Luzan > Research Assistant > Department of Biology > University of Louisiana at Lafayette > Phone (Office): 482-5056 > > _______________________________________________ > Microbio mailing list > Microbio@net.bio.net > http://www.bio.net/biomail/listinfo/microbio > > Tanya, I would agree with using sterile selica, another option you have - just dry your plate for a while. I guess it will be rather enough to leave 1.5% agar plates overnight. Cheers. Sasha. PS I could not reach your email box, sorry. -- View this message in context: http://www.nabble.com/Problem-with-cultivation-on-anaerobic-solid-media-tp13756475p16246239.html Sent from the Bio.net - Microbio mailing list archive at Nabble.com. From limbic_lesion from hotmail.com Mon Mar 24 15:06:32 2008 From: limbic_lesion from hotmail.com (N10) Date: Mon Mar 24 15:57:50 2008 Subject: [Microbiology] Problem with cultivation on anaerobic solid media References: <20071114195842.M68778@louisiana.edu> Message-ID: Hi A silica gel envelope helps N10 "Mad Twins Dad" wrote in message news:mailman.1195.1206380447.2451.microbio@net.bio.net... > > > > Luzan Tatiana wrote: >> >> In my lab we use hungate bottles only for the liquid anaerobic media and >> bi- >> layer solid media. We have to do inoculations on solid meida only using >> Petri dishes in the anaerobic jar supplemented with gas-packs or >> controlled >> atmosphere anaerobic chamber. THere is a problem in the chamber: the cag >> mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While >> incubating plates inside, the media dries up very quickly and an >> enourmous >> condensation is forming! IT is almost a lake of water there. >> >> IS there any product which would absorb an acess condensate? >> Thanks a lot >> >> -- >> >> Tanya Luzan >> Research Assistant >> Department of Biology >> University of Louisiana at Lafayette >> Phone (Office): 482-5056 >> >> _______________________________________________ >> Microbio mailing list >> Microbio@net.bio.net >> http://www.bio.net/biomail/listinfo/microbio >> >> > > Tanya, I would agree with using sterile selica, > another option you have - just dry your plate for a while. > I guess it will be rather enough to leave 1.5% agar plates overnight. > > Cheers. > > Sasha. > > PS I could not reach your email box, sorry. > -- > View this message in context: > http://www.nabble.com/Problem-with-cultivation-on-anaerobic-solid-media-tp13756475p16246239.html > Sent from the Bio.net - Microbio mailing list archive at Nabble.com. > From DrKJRoberts from gmail.com Mon Mar 31 12:57:37 2008 From: DrKJRoberts from gmail.com (DrKJRoberts@gmail.com) Date: Mon Mar 31 13:34:59 2008 Subject: [Microbiology] 59th Annual International Science and Engineering Fair - Call For Judges Message-ID: <3c59860f-7e70-4814-8072-f410433e9980@d62g2000hsf.googlegroups.com> The 59th ISEF will be held this year in Atlanta, Georgia May 11th-17th. I am looking for volunteers to serve as Grand Awards Judges in the Microbiology Category. Last year's ISEF in Albuquerque, New Mexico, over 45 different countries sent 1510 secondary school finalists to compete. We expect the same turn out at this year's Fair. I am looking for judges to choose the top finalists who will compete for thousands of dollars in prizes and awards. Grand Awards Judges will arrive in Atlanta on Tuesday, May 13 for orientation and pre-judging and will judge on Wednesday, May 14. This is the premier worldwide science research competition for high school students grades 9-12 and we need your support. If you are interested in serving as a judge at this year's ISEF, please go to the following website and register: http://www.intelisef2008.org/judging.html If you need more information, don't hesitate to contact me at drkjroberts@gmail.com Karl J. Roberts, Ph.D. Co-Chair, Microbiology Division International Science and Engineering Fair