From b.mckay from griffith.edu.au Thu May 1 02:23:51 2008 From: b.mckay from griffith.edu.au (Belinda Mckay) Date: Thu May 1 10:34:16 2008 Subject: [Microbiology] irgasan Message-ID: Can you tell me if there is any evidence of Irgasan causing contact dermatitis? Dr Belinda McKay Griffith University Nathan Brisbane QLD 4111 Australia Email: B.McKay@griffith.edu.au From medanrc from yahoo.com Fri May 2 04:02:55 2008 From: medanrc from yahoo.com (mohamed eda) Date: Fri May 2 11:47:43 2008 Subject: [Microbiology] cellulose decomposers Message-ID: <541229.21058.qm@web65514.mail.ac4.yahoo.com> Dear all Hello, I need to isolate the thermophilic cellulose decomposing microorganisms,if any one can help by sending media composition and isolation procedure. thanks in advance Mohamed Eida Mohamed Eida Agricultural Microbiology Dept. National Research Center, Egypt Hiroshima University Plant environment Graduate School of Biosphere Science Assessment of Environmental Dynamics Dept. Plant Environmental Science Lab. --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From limbic_lesion from hotmail.com Sat May 3 11:01:26 2008 From: limbic_lesion from hotmail.com (N10) Date: Sat May 3 12:48:22 2008 Subject: [Microbiology] Re: cellulose decomposers References: Message-ID: Google has 11400 references on this topic N10 "mohamed eda" wrote in message news:mailman.71.1209746863.17814.microbio@net.bio.net... > Dear all Hello, > I need to isolate the thermophilic cellulose decomposing > microorganisms,if any one can help by sending media composition and > isolation procedure. > thanks in advance > Mohamed Eida > > > Mohamed Eida > Agricultural Microbiology Dept. > National Research Center, Egypt > Hiroshima University > Plant environment > Graduate School of Biosphere Science > Assessment of Environmental Dynamics Dept. > Plant Environmental Science Lab. > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. From medanrc from yahoo.com Sun May 4 10:31:24 2008 From: medanrc from yahoo.com (mohamed eda) Date: Sun May 4 13:04:18 2008 Subject: [Microbiology] clarification Message-ID: <775009.22019.qm@web65504.mail.ac4.yahoo.com> I actually need to isolate some microorganisms that can degrade cellulose during composting process which can do this process at temperature degree of 80 degrees or more. thanks Mohamed Eida Agricultural Microbiology Dept. National Research Center, Egypt Hiroshima University Plant environment Graduate School of Biosphere Science Assessment of Environmental Dynamics Dept. Plant Environmental Science Lab. --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From stephan.bathe from iba.uka.de Mon May 5 07:03:22 2008 From: stephan.bathe from iba.uka.de (Stephan Bathe) Date: Mon May 5 10:24:03 2008 Subject: [Microbiology] Ciprofloxacin stocks Message-ID: <001801c8aea8$042dac60$0c890520$@bathe@iba.uka.de> Hi, does anyone know how to produce stable, dissolved ciprofloxacin stock solutions around 1 mg/ml? Thanks for any suggestions. Stephan From dayakarbabu from gmail.com Wed May 14 09:17:58 2008 From: dayakarbabu from gmail.com (alex) Date: Wed May 14 11:26:50 2008 Subject: [Microbiology] regarding masters Message-ID: <3a178eae-bbf7-46e0-9902-6c4da281ed5d@b64g2000hsa.googlegroups.com> Hi, I have completed my Bachelors of science in Microbiology (BSc) (10 + 2+ 3) from Nagarjuna university India. I want to do my MSc. I am unable pickup right course, because i dont have any idea about the courses and their future. Can some one please suggest one course ex. Msc general microbiology, Msc industrial micro, Msc medical micro, Msc applied micro, Msc forensic science, Msc human genetics and etc.. Please suggest one university which is offering that course and fee structure of that course, and also kindly tell me in which country i will good exposure and better job. I am ready to do my Msc(MS) out side the india also. all i want is better life and good job. Thanks in advance. Day From pwangiwan from appsci.unitech.ac.pg Tue May 13 21:47:59 2008 From: pwangiwan from appsci.unitech.ac.pg (pwangiwan) Date: Wed May 14 11:26:54 2008 Subject: [Microbiology] API 20C AUX YEAST IDENTIFICATION KIT Message-ID: <000601c8b56c$ec00a5e0$982101ca@appsci33152> Der Sir/Madam, I would like to know more about this yeast identification kit. Could you also please send me quotation for one of this kit. Thank you. Pelis Wangiwan Senior Technical Officer - Food Microbiology Dept. of Applied Sciences Papua New Guinea University of Technology Private Mail Bag, LAE, Morobe Province Papua New Guinea. From mike.palermo from insightbb.com Thu May 15 21:51:25 2008 From: mike.palermo from insightbb.com (MICHAEL T. PALERMO, JD, CFP) Date: Fri May 16 11:13:53 2008 Subject: [Microbiology] Hemoglobin detection at 540 nm or 430 nm Message-ID: <011501c8b6ff$bb302730$6401a8c0@acer6fa91de09f> I am writing a novel that I want to be (somewhat) technically precise. In referring to the wavelengths above (reference to which I stumbled upon in an online message post), I assume you are talking about absorption peaks? Would you be kind enough to tell me what sort of sprectrophotometry you are talking about? Thanks very much. Mike Palermo From txl8326 from louisiana.edu Fri May 16 13:10:45 2008 From: txl8326 from louisiana.edu (Luzan Tatiana) Date: Fri May 16 20:25:30 2008 Subject: [Microbiology] Re: Microbio Digest, Vol 36, Issue 7 In-Reply-To: <200805161706.m4GH6eO06847@net.bio.net> References: <200805161706.m4GH6eO06847@net.bio.net> Message-ID: <20080516180812.M69368@louisiana.edu> Hello I really need suggestion or,even better, the reference to the method of determination of acetate with visible light chromatography in sediment samples. CUrrently I am trying to determine it with ion chromatography, but my machine is down, and I am trying to fix it. Thanks Tanya On Fri, 16 May 2008 12:06:40 -0500 (EST), microbio-request wrote > Send Microbio mailing list submissions to > microbio@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/microbio > or, via email, send a message with subject or body 'help' to > microbio-request@net.bio.net > > You can reach the person managing the list at > microbio-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Microbio digest..." > > Today's Topics: > > 1. Hemoglobin detection at 540 nm or 430 nm > (MICHAEL T. PALERMO, JD, CFP) > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 15 May 2008 22:51:25 -0400 > From: "MICHAEL T. PALERMO, JD, CFP" > Subject: [Microbiology] Hemoglobin detection at 540 nm or 430 nm > To: > Message-ID: <011501c8b6ff$bb302730$6401a8c0@acer6fa91de09f> > Content-Type: text/plain; charset="us-ascii" > > I am writing a novel that I want to be (somewhat) technically > precise. In referring to the wavelengths above (reference to which > I stumbled upon in an online message post), I assume you are talking > about absorption peaks? > > Would you be kind enough to tell me what sort of sprectrophotometry > you are talking about? > > Thanks very much. > > Mike Palermo > > ------------------------------ > > _______________________________________________ > Microbio mailing list > Microbio@net.bio.net > http://www.bio.net/biomail/listinfo/microbio > > End of Microbio Digest, Vol 36, Issue 7 > *************************************** -- Tanya Luzan Research Assistant Department of Biology University of Louisiana at Lafayette Phone (Office): 482-5056 From senaatlaw from yahoo.com Fri May 16 15:04:25 2008 From: senaatlaw from yahoo.com (senaatlaw Atlaw Woldeyohanes) Date: Sat May 17 13:34:42 2008 Subject: [Microbiology] Reference Message-ID: <634891.29947.qm@web63114.mail.re1.yahoo.com> Dear Sir, I have isolated a Microorganism, Gram-negative Coccobacillus Streaked it on MacConkey agar, pink colonies started to grow before eight hours. Today after 24 hrs, it is Lactose fermenting, good pink colonies, and is Oxidase positive too, I thought it would be from Enterobactericeae, but it is rare to be Oxidase positive or unless one of the rare ? I only have the basic id. Material, and I am going to send it for identification to another Lab. But I am so curious what can it be, I try to find out on my Diagnostic Microbiol. Book, nothing matches at this moment I may have to see more. Mac, Positive and Oxidase positive, if there is some common microorganism which matches this or a good reference, would you please let me know. Thanks Regards From farrlarr from isu.edu Sat May 17 16:53:52 2008 From: farrlarr from isu.edu (Larry Farrell) Date: Sun May 18 12:30:49 2008 Subject: [Microbiology] Re: Reference In-Reply-To: References: Message-ID: <69541$482f546c$18509@news.teranews.com> Bergey's Manual of Determinitive Bacteriology. senaatlaw Atlaw Woldeyohanes wrote: > Dear Sir, > > I have isolated a Microorganism, Gram-negative Coccobacillus > Streaked it on MacConkey agar, pink colonies started to grow before eight hours. > Today after 24 hrs, it is Lactose fermenting, good pink colonies, > and is Oxidase positive too, I thought it would be from Enterobactericeae, but it is rare to be Oxidase positive or unless one of the rare ? > I only have the basic id. Material, and I am going to send it for identification to another Lab. > But I am so curious what can it be, I try to find out on my Diagnostic Microbiol. Book, nothing matches at this moment I may have to see more. > Mac, Positive and Oxidase positive, if there is some common microorganism which matches this or a good reference, would you please let me know. > > Thanks > > Regards > > > ** Posted from http://www.teranews.com ** From yjgent from nospamcox.net Sat May 17 20:26:54 2008 From: yjgent from nospamcox.net (John Gentile) Date: Sun May 18 12:30:54 2008 Subject: [Microbiology] Re: Reference References: Message-ID: <2008051721265416807-yjgent@nospamcoxnet> On 2008-05-16 16:04:25 -0400, senaatlaw Atlaw Woldeyohanes said: > Dear Sir, > > I have isolated a Microorganism, Gram-negative Coccobacillus > Streaked it on MacConkey agar, pink colonies started to grow before > eight hours. > Today after 24 hrs, it is Lactose fermenting, good pink colonies, > and is Oxidase positive too, I thought it would be from > Enterobactericeae, but it is rare to be Oxidase positive or unless one > of the rare ? > I only have the basic id. Material, and I am going to send it for > identification to another Lab. > But I am so curious what can it be, I try to find out on my > Diagnostic Microbiol. Book, nothing matches at this moment I may have > to see more. > Mac, Positive and Oxidase positive, if there is some common > microorganism which matches this or a good reference, would you please > let me know. > > Thanks > > Regards > > > We're going to need a bit more information. Is this a human isolate, animal or environmental? Did you do basic id media like TSI, Urea, motility, Indole? Are you sure it is a pure isolate? Make sure you streak for isolation, select a single colony. Is your oxidase reagent fresh? I know of only one human isolate that could (<3%) be lactose pos and oxidase pos. Also be careful about your Gram's stain morphology. -- John Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net From bactitech from nospamhortonsbay.com Sun May 18 11:00:29 2008 From: bactitech from nospamhortonsbay.com (JEDilworth) Date: Sun May 18 12:30:58 2008 Subject: [Microbiology] Re: Reference References: Message-ID: Are you performing oxidase off of the Mac? If you are, it is NOT accurate off of this medium. You MUST perform oxidase on a nonselective medium like sheep blood agar or TSA plate. The pink color of the organism and medium renders this plate off-limits for oxidase testing. Get back to us when you perform the correct oxidase. Can you do indole testing off of your blood agar plate too while you've got a fresh isolate? You must have a blood agar plate (tryptophane source) to perform indole testing directly from plate, or a medium designed to test for indole. Make SURE you've streaked well for isolation. This is more important than anything. Make sure you're working with a one colony isolate. These are basic tests for lactose fermenters and will tell you where to go after you do these. Bergey's manual is an excellent choice for reference, but you MUST correctly perform these tests first. Judy Dilworth, M.T. (ASCP) Microbiology "senaatlaw Atlaw Woldeyohanes" wrote in message news:mailman.120.1211049307.3533.microbio@net.bio.net... > Dear Sir, > > I have isolated a Microorganism, Gram-negative Coccobacillus > Streaked it on MacConkey agar, pink colonies started to grow before > eight hours. > Today after 24 hrs, it is Lactose fermenting, good pink colonies, > and is Oxidase positive too, I thought it would be from > Enterobactericeae, but it is rare to be Oxidase positive or unless one > of the rare ? From pcharusa from bioeng.ucsd.edu Mon May 19 13:02:16 2008 From: pcharusa from bioeng.ucsd.edu (Pep Charusanti) Date: Mon May 19 13:57:01 2008 Subject: [Microbiology] extracting compounds from agar plates Message-ID: Hello everybody, Does anybody have a protocol on how to extract compounds from agar? I am growing bacteria on plates and they are secreting compounds (probably metabolites) into the agar that I would like to isolate. I tried growth in liquid culture, but by HPLC analysis of the supernatant I know this does not lead to production of the compounds I'm interested in. From limbic_lesion from hotmail.com Mon May 19 16:52:00 2008 From: limbic_lesion from hotmail.com (N10) Date: Mon May 19 20:25:03 2008 Subject: [Microbiology] Re: extracting compounds from agar plates References: Message-ID: "Pep Charusanti" wrote in message news:mailman.134.1211223442.3533.microbio@net.bio.net... > Hello everybody, > > Does anybody have a protocol on how to extract compounds from agar? I am > growing bacteria on plates and they are secreting compounds (probably > metabolites) into the agar that I would like to isolate. I tried growth > in liquid culture, but by HPLC analysis of the supernatant I know this > does not lead to production of the compounds I'm interested in. > Hello Pep What group of compounds are you interested in ? regards N10 From axp955 from psu.edu Tue May 20 09:43:02 2008 From: axp955 from psu.edu (AMRITA PURI) Date: Tue May 20 09:55:35 2008 Subject: [Microbiology] Isolating Coprinus sp. Message-ID: <1211294582l.630948l.0l@psu.edu> Hello everyone I want to isolate a fungal species Coprinus sp. from compost. Does anyone have any idea how I can go about doing that or where I could find the information? Thanks Regards AP From rohitash13 from gmail.com Thu May 22 01:22:23 2008 From: rohitash13 from gmail.com (rOHITASH jAMWAL) Date: Thu May 22 10:00:34 2008 Subject: [Microbiology] Isolation of compounds from sillica gel Message-ID: Hello folks I am trying to extract my compounds from TLC plate(Sillica gel)...I have used ethanoloc extracts for assay. Could anyone please guide me how to isolate active compounds from sillica gel. Rohitash rohitash13@gmail.com From tshakanl.SCI from ufs.ac.za Thu May 22 05:16:52 2008 From: tshakanl.SCI from ufs.ac.za (Nombulelo Tshaka) Date: Thu May 22 10:00:38 2008 Subject: [Microbiology] Good morning Message-ID: <483564AB.8D5D.00E6.0@ufs.ac.za> Good morning Can you please tell me why sodium phosphate buffer's significance is in DNA extraction. Why is the sticky end used often in Restriction digestion and not blunt end. what is a decent metagenomic library. _____________________________________________________________________ University of the Free State: This message and its contents are subject to a disclaimer. Please refer to http://www.ufs.ac.za/disclaimer for full details. Universiteit van die Vrystaat: Hierdie boodskap en sy inhoud is aan 'n vrywaringsklousule onderhewig. Volledige besonderhede is by http://www.ufs.ac.za/vrywaring beskikbaar. _____________________________________________________________________ From oscar.martinez from syva.es Thu May 22 05:33:19 2008 From: oscar.martinez from syva.es (Oscar Martinez Arias) Date: Thu May 22 10:00:53 2008 Subject: [Microbiology] mucus of C. sordellii Message-ID: Hello, I am interested in separating or eliminating the mucus secreted by C. sordellii from the rest of the product of its fermentation, without a centrifugation step. Is there any enzymatic or chemical solution to this problem? Many thanks in advance. Oscar Martinez, DVM. From txl8326 from louisiana.edu Thu May 22 13:50:43 2008 From: txl8326 from louisiana.edu (Luzan Tatiana) Date: Thu May 22 17:01:59 2008 Subject: [Microbiology] ultramicrocentrifugation for archeal cells Message-ID: <20080522184907.M30546@louisiana.edu> Hello, Can anyone suggest the parameters for ultramicrocentrifugation in order to isolate the smallest archeal cell fraction? Thanks a lot Tanya Luzan, student -- Tanya Luzan Research Assistant Department of Biology University of Louisiana at Lafayette Phone (Office): 482-5056 From limbic_lesion from hotmail.com Thu May 22 15:42:37 2008 From: limbic_lesion from hotmail.com (N10) Date: Thu May 22 17:02:27 2008 Subject: [Microbiology] Re: Isolation of compounds from sillica gel References: Message-ID: "rOHITASH jAMWAL" wrote in message news:mailman.180.1211468470.3533.microbio@net.bio.net... > Hello folks > I am trying to extract my compounds from TLC plate(Sillica gel)...I have > used ethanoloc extracts for assay. > > Could anyone please guide me how to isolate active compounds from sillica > gel. > > Rohitash > rohitash13@gmail.com Hi Rohitash What type or class of compound do you think you might be extracting ? Is an ethanolic extractant the best choice ? Look to the composition of the mobile phase which produced the target band in the first place. The chemical composition of that should indicate the nature of a suitable extractant for you Also how do you know that your current approach has been unsuccesfull. ? What you mean by an active compound ? Bets N10 ;) From ruthsiele from yahoo.com Mon May 26 12:03:11 2008 From: ruthsiele from yahoo.com (RUTH SIELE) Date: Tue May 27 12:47:42 2008 Subject: [Microbiology] Synthetic brakish water recepi Message-ID: <744220.91104.qm@web45205.mail.sp1.yahoo.com> Hi all, Does any one have a clue regarding synthetic brackish water recepi? thank you so much for your help in advance, Ruth From mhinsdal from wfubmc.edu Thu May 29 13:05:33 2008 From: mhinsdal from wfubmc.edu (Mark Hinsdale) Date: Thu May 29 16:22:18 2008 Subject: [Microbiology] Pre-reduced media preparation Message-ID: <61135F0455D33347B5AAE209B903A3042374656E@EXCHVS2.medctr.ad.wfubmc.edu> Mr.Randolph,We are interested in a quote for anaerobic media for growing Oxalobactor with resazurin as an indicator of O2 production. Please email or call me at 336-716-1371 to discuss this. Thanks,Mark Hinsdale ,Wake Forest University Health Sciences